Simulated physiological oocyte maturation (SPOM) improves developmental competence of in vitro produced goat embryos

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dc.contributor.authorSuresh, Ashitha
dc.contributor.authorShukla, MK
dc.contributor.authorKumar, Dharmendra, et al.,
dc.contributor.authorTANUVAS
dc.date.accessioned2021-12-24T11:53:40Z
dc.date.available2021-12-24T11:53:40Z
dc.date.issued2021
dc.descriptionTNV_THER_2021_172(1)en_US
dc.description.abstractThe effect of simulated physiological oocyte maturation on the developmental competence, reactive oxygen species production and apoptosis rate of in vitro produced goat embryos were studied in the present experiment. Oocytes and spermatozoa were recovered from ovaries and epididymis, respectively, procured from a local small animal abattoir. The oocytes aspirated from the ovaries were allocated into two groups, control (subjected to routine in vitro maturation, fertilization and culture) and simulated physiological oocyte maturation (SPOM) group (subjected to prematuration, followed by routine in vitro maturation, fertilization and culture). The SPOM group showed a significantly (p < 0.05) higher maturation and blastocyst rates (90.60 ± 0.46% and 29.09 ± 2.59%, respectively) as compared to the control group (85.29 ± 0.98% and 24.09 ± 1.08%). The intensity of reactive oxygen species of the embryos in the control group (14.98 ± 0.83 pixels/embryo) was significantly (p < 0.05) higher than the SPOM group (9.60 ± 0.76 pixels/embryo). The apoptosis rate was also significantly (p < 0.05) higher in the embryos of the control group (9.18 ± 1.07%) as compared to the SPOM group (5.71 ± 0.90%). In conclusion, the simulated physiological oocyte maturation system significantly increases the developmental competence of the oocytes and decreases the intensity of reactive oxygen species and embryonic apoptosis in abattoir derived goat embryos.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810179877
dc.keywordsIn vitro fertilization Simulated physiological oocyte maturation Goats Developmental competence Reactive oxygen species Apoptosisen_US
dc.language.isoEnglishen_US
dc.pages1en_US
dc.subjectVeterinary Scienceen_US
dc.titleSimulated physiological oocyte maturation (SPOM) improves developmental competence of in vitro produced goat embryosen_US
dc.title.alternativeTheriogenologyen_US
dc.typeArticleen_US
dc.volume172en_US
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