EFFECT OF SUPPLEMENTATION OF GLUTATHIONE AND HONEY IN SKIM MILK BASED EXTENDERS FOR PRESERVATION OF BOER BUCK SEMEN AT REFRIGERATION TEMPERATURE
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Date
2019-12
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KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR
Abstract
Addition of 1% honey in skim milk glutathione extender increased motile sperm
percentage while addition of 1% honey in skim milk extender reduced it. Addition of 1%
honey in skim milk glutathione extender reduced immotile sperm percentage in contrast
addition of 2% honey in skim milk extender increased it. Addition of 1% honey in skim
milk glutathione extender improved rapid progressive sperm percentage whereas addition
of 2% honey in skim milk extenders reduced it. Slow progressive sperm percentage was
lower in skim milk glutathione extender whereas addition of 2% honey in skim milk
extender increased it. Non-progressive sperm percentage was lower in skim milk
glutathione extender however addition of 1% honey in it increased it. Addition of 1%
honey in skim milk glutathione extender showed higher sperm curvilinear velocity, sperm
straight line velocity and sperm average path velocity however addition of 2% honey in
skim milk extender reduced them. Addition of 1% honey in skim milk extender reduced
sperm linearity percentage, sperm wobble percentage and sperm straightness percentage
whereas the same were higher in skim milk glutathione extender. Addition of honey (1% or
2%) did not show effect on sperm beat cross frequency and amplitude of lateral sperm head
displacement whereas the same were higher in skim milk glutathione extender and lower in
skim milk extender. Addition of 1% honey in skim milk glutathione extender increased
sperm dance whereas 2% honey addition in skim milk extender lowered it. Addition of 2%
honey in skim milk extender increased sperm dance mean whereas reduced in skim milk
glutathione extender at 72 h of refrigeration.