STUDIES ON RESPIRATORY INFECTION IN SHEEP WITH EMPHASIS ON MYCOPLASMA
Loading...
![Thumbnail Image](assets/images/Item.jpg)
Date
2017-11
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR
Abstract
This study was conducted to record the clinical signs suggestive of
mycoplasmoses, changes in haematological parameters, isolation, and identification and
to screen the sheep for mycoplasma antibodies. The clinical signs in the flocks which
have been studied were mostly of upper respiratory tract infection like nasal discharge
(74), followed by conjunctivitis (17), abortion (15), mastitis (08), arthritis (08) and
corneal opacity (03) in different flocks 2with the percentage of 41.11, 9.44, 8.33, 4.44,
4.44 and 1.66, respectively. There was stastically significant elevation in the TLC in the
mycoplsma suspected sheep when compared to healthy animals. Filamentous and whirl
pool like structure when agitated were noticed in most of the liquid media. Three types of
colonies were noticed in the present study which consisted of central nipple and granular
periphery identified as Mycoplasma agalactiae, vacuolated and very big size colonies
were identified as Mycoplasma ovipneumoniae and typical fried egg (nipple shaped)
colonies were identified as other mycoplasma. A total of 43 isolates were tentatively
identified as mycoplasma based on cultural methods and biochemical tests which
comprised of 23.88%. The different species of mycoplasma as identified by biochemical
tests and confirmed by PCR were M. ovipneumoniae (32), M. arginini (06), M.
agalactiae (03) and M. capricolum (02) which comprised of 74.41, 13.95, 6.97 and 4.65
per cent, respectively. A total of 360 sheep were screened using Mycoplasma
ovipneumoniae particulate antigen and highest seropositive animals were noticed in the
lambs less than six months old (92.22%) followed by the sheep aged more than three
years 65.58%. The other middle age group sheep ie, 6 month-1 year and 1-3 year had
seropositivity of 28.88% and 47.77%, respectively. A significant difference was noticed
in the number of ELISA positive cases in different age groups.