Rapid and Sensitive Faecal based PCR Assays for the Detection of Parvoviruses in Laboratory Rats
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Date
2020-08
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Abstract
Health status of laboratory rats indicates its suitability for the researches. They are
prone to develop viral infections of subclinical nature caused by parvoviruses,
which affects the research results adversely; Hence Implementation of health
monitoring protocol is essential at the level of breeding colony itself. This study
was intended to develop rapid and sensitive fecal-PCR assays to detect infections
of multiple species of Parvoviruses affecting rats namely, Rat Minute Virus,
Toolan’s Parvo Virus, Rat Parvo Virus and Kilham’s Rat Virus as an alternate
approach to serology based health monitoring in a lab animal breeding unit. All
the primers detected only in the presence of the respective templates. PCR assays
of RMV and TPV consistently amplified as little as 40 fg and that of RPV and
KRV consistently amplified as little as 4 fg of plasmid DNA. Specificity and
sensitivity assays indicate that the PCR assays may be useful as diagnostic tools
for rapid detection of natural acute viral infections. Further analysis of the primers
in the positive laboratory animal colony is essential.
Description
TNV_IJCMAS_2020_9(8)3506-3512
Keywords
Veterinary Science