Rapid and Sensitive Faecal based PCR Assays for the Detection of Parvoviruses in Laboratory Rats

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dc.contributor.authorSrinivasan, MR
dc.contributor.authorVijay, K
dc.contributor.authorRamesh, S
dc.contributor.authorKaruppannan, AK
dc.contributor.authorKrishnamohan Reddy, Y
dc.contributor.authorTANUVAS
dc.date.accessioned2020-09-04T08:48:22Z
dc.date.available2020-09-04T08:48:22Z
dc.date.issued2020-08
dc.descriptionTNV_IJCMAS_2020_9(8)3506-3512en_US
dc.description.abstractHealth status of laboratory rats indicates its suitability for the researches. They are prone to develop viral infections of subclinical nature caused by parvoviruses, which affects the research results adversely; Hence Implementation of health monitoring protocol is essential at the level of breeding colony itself. This study was intended to develop rapid and sensitive fecal-PCR assays to detect infections of multiple species of Parvoviruses affecting rats namely, Rat Minute Virus, Toolan’s Parvo Virus, Rat Parvo Virus and Kilham’s Rat Virus as an alternate approach to serology based health monitoring in a lab animal breeding unit. All the primers detected only in the presence of the respective templates. PCR assays of RMV and TPV consistently amplified as little as 40 fg and that of RPV and KRV consistently amplified as little as 4 fg of plasmid DNA. Specificity and sensitivity assays indicate that the PCR assays may be useful as diagnostic tools for rapid detection of natural acute viral infections. Further analysis of the primers in the positive laboratory animal colony is essential.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810150825
dc.keywordsParvovirus, Fecal PCR assay, Health monitoringen_US
dc.language.isoenen_US
dc.pages3506-3512en_US
dc.relation.ispartofseries;8
dc.subjectVeterinary Scienceen_US
dc.titleRapid and Sensitive Faecal based PCR Assays for the Detection of Parvoviruses in Laboratory Ratsen_US
dc.title.alternativeInternational Journal of Science, Environment and Technologyen_US
dc.typeArticleen_US
dc.volume9en_US
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