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Dr. Y. S. Parmar University of Horticulture & Forestry, Solan
Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, was established on 1st December, 1985 with the objective to promote education, research and extension education in the fields of Horticulture, Forestry and allied disciplines. Late Dr. Yashwant Singh Parmar, the first Chief Minister and the architect of Himachal Pradesh perceived the importance of Horticulture and Forestry to develop and improve the State economy which led to the establishment of this University. Its history lies in erstwhile Himachal Agricultural College, Solan, established in 1962 and affiliated to the Panjab University. It became one of the campuses of Agriculture Complex of Himachal Pradesh University on its formation in 1970. Consequent upon the establishment of Himachal Pradesh Krishi Vishvavidyalaya in 1978, this campus became its Horticulture Complex and finally in 1985, assumed the status of a State University, being the only University in the country engaged exclusively in teaching, research and extension in Horticulture and Forestry.
The University is located at Nauni in Solan District of Himachal Pradesh, 13 km from Solan on Solan-Rajgarh Road, at an elevation of 1300 metres above mean sea level. Solan town is situated on national highway (NH-22) and is well connected by train and bus services.
The University has four constituent colleges, out of which, two are located at the main campus Nauni, one for horticulture and the other for forestry, having 9 and 7 departments, respectively. The third College i.e., College of Horticulture & Forestry is located at Neri in Hamirpur District on Nadaun-Hamirpur state highway, about 6 Km from Hamirpur town and is well connected with bus service. The college offers three Undergraduate Degree Programmes i.e. BSc (Hons.) Horticulture, BSc (Hons.) Forestry and B. Tech. Biotechnology and MSc degree programme in a few subjects. The fourth college i.e. College of Horticulture and Forestry, Thunag (Mandi) is located at Thunag District Mandi. This college offer BSc (Hons.) Horticulture and BSc (Hons.) Forestry degree programme. In addition, there are five Regional Research Stations, 12 Satellite Stations and five Krishi Vigyan Kendras (KVKs) situated in different zones of the State.
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ThesisItem Open Access B gASSESSMENT OF AIR AND WATER QUALITY UNDER DOMINANT LANDUSES IN MID HILLS OF HIMACHAL PRADESH(UHF,NAUNI, 2019-11) SHARMA, NITIKA; BHARDWAJ, S KABSTRACT The present investigation entitled “Assessment of Air and Water Quality under Dominant Land Uses in Mid Hills of Himachal Pradesh” was conducted during the years 2017 and 2018 in the Department of Environmental Science, Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh. The study was undertaken to identify the impacts of dominant land uses namely urban, peri urban, agriculture and forest on air and water quality. The impact was studied during pre and post monsoon season for air quality and under three seasons for water quality. The study was conducted throughout the mid hill conditions by taking six locations which were considered as replications. Accordingly, there were eight and twelve treatment combinations in air and water quality monitoring experiments which were replicated six times under Randomized Block Design (factorial). Geospatial mapping of air and water quality status under different land uses was done using ERDAS software. Survey was also conducted to identify the pollution causing practices in mid hills. The study pointed out that the air quality parameters like PM10, VOCs, SO2 and NO2 were significantly influenced by land uses of mid hills which ranged from 61.0 to 93.0 µg m-3, 1.20 to 6.80 µg m-3, 1.45 to 7.57 µg m-3 and 11.68 to 40.43 µg m-3, respectively. However, all the parameters were within the permissible limits. Similarly, land uses also exerted significant influence on surface and ground water quality. The physical parameters like pH, EC, TDS, Turbidity ranged from 6.45 to 6.77, 0.246 to 0.453 dSm-1, 64.0 to 229.17 mg l-1, 2.95 to 6.98 NTU respectively. The chemical parameters like BOD, COD, DO, Ca, Mg, NO3-, Cl-ranged from 0.67 to 3.27 mg l-1, 14.2 to 21.6 mg l-1, 2.48 to 5.75 mg l-1, 32.33 to 139.50 mg l-1, 3.13 to 12.67 mg l-1, 0.64 to 3.67 mg l-1, 8.12 to 32.67 mg l-1, respectively. The trace elements like Cd, Cr, Pb, Zn, As ranged from 0.00 to 0.036 mg l-1, 0.001to 0.059 mg l-1, 0.009 to 0.375 mg l-1, 0.002 to 0.325 mg l-1, 0.00 to 0.005 mg l-1 respectively. The physical-chemical parameters of water quality were within the permissible limits prescribed by BIS and CPCB except for calcium, chromium and lead. The air quality index was in the range of 0-50 and considered good. The water quality index computed by considering nine quality parameters was in the range 26-50 under forest land use and was in good category. Whereas, this index was in the range of 51-75 under urban, peri urban and agriculture land use and was in poor category. The water quality index was in the descending qualityorder of: forest> peri urban > urban> agriculture. The air and water quality also varied with the seasons in the region.The deterioration of water quality was more in monsoon season. In mid hills unscientific disposal of waste water and release of untreated sewage was identified as pollution causing practice in surface water sources under urban and peri urban land use. Injudicious use of chemicals was major pollution causing practice under agriculture land use. The increased vehicular activity, road expansion and construction activities in case of urban and peri urban, forest fires and burning of residues under forest and agriculture land use were the major air pollution causing practices in mid hills. The study inferred that dominant land uses of mid hills have started impacting air and water quality due to various wrong practices. However, the effects were more pronounced in case of surface water quality therefore, in order to maintain the quality of these important natural resources within the safe limits there is urgent need to promote eco friendly practices while managing the land uses of this region.ThesisItem Open Access CHARACTERIZATION OF CULTURABLE BACTERIAL ENDOPHYTES OF CHRYSANTHEMUM (Dendranthema grandiflora Tzvelev) AND THEIR EFFICACY FOR PLANT GROWTH PROMOTION(UHF,NAUNI, 2019-11) SHARMA, SHILPA; CHAUHAN, ANJALIABSTRACT Chrysanthemum (Dendranthema grandiflora Tzvelev) belongs to family Asteraceae and is a popular flower crop suitable for both pot culture and bedding purposes. The quality of flowers is greatly influenced by the quantity as well as sources of nutrients. Presently, these nutrients are supplied through chemical fertilizers. The escalating prices of chemical fertilizers and their indiscriminate use has not only adversely affects the soil health and environment but also reduces the productivity of crops. The situation emphasized the need for developing alternate production system that is eco-friendly and is more judicious in maintaining soil health. So, the present investigations were carried out to characterize and evaluate the effects of PGPB isolated from chrysanthemum plant (roots, stem and leaf) samples grown under organic and commercial cultivation. A total of 143 purified isolates were selected on the basis of PGP traits and morphological characterization. From the total, 44 (16 organic and 28 inorganic) isolates were selected on the basis of their efficacy to have maximum plant growth promoting traits like P-solubilization, growth on nitrogen free medium, siderophore, auxin, HCN production and antagonism against Pythium ultimum, Rhizoctonia solani and Fusarium oxysporumunderlaboratoryconditions.The genetic diversity based on 16S r DNA sequence analysis and BLASTn search revealed the identity of 44 strains belonging to diverse genera viz.,Bacillus, Pseudomonas, Stenotrophomonas, Lysinibacillus, Micrococcus, Streptomyces, Pantoea, Klebsiella, Phyllobacterium, Serratia, Microbacterium, Cellulosimicrobium, Arthrobacter and Staphylococcus. From selected 44 isolates, seven isolates viz. Stenotrophomonas pavanii strain SRO8, Bacillus subtilis strain RDO10, Serratia marcescens strain N2S14, Bacillus subtilis strain N1S25, Serratia nematodiphila strain N1S23, Pseudomonas aeruginosa strain N2S6 and Bacillus subtilis strain N2S18 along with consortium of reference strains (Bacillus licheniformis strain KS_5+Bacillus subtilis strain KS_6) were selected for pot trial to study their efficacy for growth and development of chrysanthemum. In the pot trial experiment two best isolates were selected with different levels of NP fertilizers along with two efficient isolates (KS5 and KS6) and used for field trial using two varieties of chrysanthemum viz ‘Ajay’ and ‘Purnima’. The application of 70% RD NP + Bacillus subtilis strain RDO10 and 70% RD NP + Pseudomonas aeruginosa strain N2S6 registered maximum increase in all plant parameters i.e. plant height (cm), number of side shoots per plant, days taken to flowering, number of flowers per plant, flower size (cm), duration of flowering (days) and vase life (days). The inoculation of PGPB has also registered an increase in NPK uptake and available NPK. Thus, the selected bacterial endophytes has good prospects to be used as biofertilizer, biostimulant and bioprotectant for chrysanthemum.ThesisItem Open Access STUDIES ON ANNEXIN GENE FAMILY FROM SOLANACEOUS VEGETABLES(UHF,NAUNI, 2019-12) SHARMA, ANKITA; NATH, AMARJIT K.ABSTRACT Heat stress leads to adverse effect on Potato cultivation affecting the tuberization and the bulking process. Being a signaling molecule, it is hypothesized that annexins might play important role in tuberization under heat stress condition. Ten annexins from potato and 13 annexins from tomato were identified using bioinformatics tool. Annexin gene proteins from potato and tomato were found to have an average molecular weight of 36kDa. Both potato and tomato annexins showed differential location of the annexin genes on 12 chromosomes. Expression profiling of Potato annexin genes under heat stress conditions revealed strong up-regulation of annexins in heat tolerant potato cultivar Kufri Surya as compared to susceptible one i.e Kufri Chandramukhi indicating their role as key signaling molecule under heat stress conditions. The annexin gene StAnn1 showed a uniform pattern of elevated gene expression in both leaves and stolon under heat stress throughout the developmental stage of potato cultivar KS as compared to potato cultivar KCM. Significant increase in peroxidase, anthocyanin and proline content was observed in heat tolerant cultivar Kufri Surya on different intervals of exposure to heat stress. The content of MDA was significantly less in Kufri Surya as compared to Kufri Chandramukhi indicating much less membrane damage in the tolerant potato cultivar KS. The annexin gene StAnn1 was isolated and cloned from Kufri Surya and over expressed in heat susceptible cultivar Kufri Chandramukhi for exploiting its potential for providing heat stress tolerance in other susceptible potato cultivars. Agrobacterium mediated genetic transformation protocol was standardized for potato cultivar Kufri Chandramukhi where selective MS medium with composition containing IAA 0.042mg/L, GA33.0 mg/L, Zeatin 3.0 mg/L, NAA 0.008 mg/L, Kan 50 mg/L, Carb 250 mg/L, Cefo 100 mg/L showed maximum percentage of callus formation i.e 76 % and shoot regeneration. The best rooting was observed on selective root regeneration MS medium containing 50mg/L Kan and 0.3mg/L IAA. On exposure to in vitro salt stress, transgenic KCM plants expressing StAnn1 annexin gene, phenotypically showed better tolerance as compared to the control plants depicting its potential in providing tolerance to other abiotic stress also. A decline in number of roots, nodes/plant and plant height was observed in control shoots as compared to putative transgenic shoots.ThesisItem Open Access IN VITRO MUTAGENESIS IN GINGER (Zingiber officinale Rosc.) FOR THE SELECTION OF PLANTS RESISTANT TO Fusarium oxysporum f.sp. zingiber(UHF,NAUNI, 2019-12) SHARMA, VISHAL; THAKUR, MANISHAABSTRACT In the present investigation, an attempt has been made to develop Fusarium yellows resistant plants of ginger (Zingiber officinale Rosc.) var. using in vitro mutagenesis and shoot selection technique. In vitro propagation was done using buds as explants. Maximum in vitro bud establishment was achieved in spring season (62.85%). 0.2% HgCl2 for 5 minutes was found to be the best concentration of surface sterilant with 42.59 per cent survival. 72.85% buds established on MS medium supplemented with 1 mg/l BA and 0.1 mg/l NAA. MS medium fortified with 0.5 mg/l BA and 0.1 mg/l NAA was found to be best multiplication medium resulting in 5.2 average number of shoots per explant with average shoot length of 4.5 cm. Rooting could be achieved on the same medium after second subculture. With the increase in number of subcultures, shoot multiplication rate, average shoot length, per cent rooting, thickness of roots and average number of roots per shoot showed an increase. In vitro cultures were subjected to gamma irradiation (10-100 Gy) for in vitro mutagenesis. LD50 was calculated after every four weeks and it was calculated to be 15 Gy after 16 weeks of irradiation. The non irradiated and irradiated plantlets were hardened with 100 per cent survival rate in potting mixture comprising of soil: cocopeat: FYM (2:1:1). On biochemical evaluation of rhizomes, it was observed that essential oil content was found to be same in all the samples (2%) whereas, highest oleoresin content was found in tissue culture propagated rhizomes (3.16%) and lowest crude fibre content (5.3%) was obtained in rhizomes collected from gamma irradiated plants. HPLC studies on gingerol content showed its highest value in rhizomes produced by gamma irradiated plants. No difference in chromosome number was observed in tissue culture propagated and gamma irradiated shoots. Gamma irradiated shoots were cultured on selective medium containing different concentrations (0-20%) of fungal culture filtrate (FCF) for in vitro selection. 17.5% FCF was found to be the highest concentration on which 5% shoots survived after 1st selection cycle which reduced to 1.15% after 3rd alternate, discontinuous selection cycle. Selected shoots from 15 and 17.5% FCF were multiplied on multiplication medium. Rooted plantlets were successfully hardened with 100% survival. 46.4 and 52% plants selected at 15 and 17.5% FCF were found to be highly resistant on in vivo evaluation. SCoT and SSR analysis distinguished between FCF selected, tissue culture propagated and gamma irradiated plants. In SSR analysis, two unique bands were obtained in plantlets selected on 17.5% FCF. On sequencing, the band obtained with GIN6 showed 98% homology with disease resistance protein-like gene CC-NBS-LRR from clone ZwP627 and band obtained with primer GIN9 showed 97% homology with same gene from clone ZoP620 of Z. officinale.After in vivo bioassay, SSR analysis of selected highly resistant plants again confirmed the unique bands with both the primers and the unique DNAsequences obtained have been submitted to NCBI under accession number MN497252 and MN497253 and will be published in GenBank after complete processing. Therefore, in the present study we have successfully developed Fusarium yellows resistant plants of ginger (Zingiber officinale Rosc.) var. Himgiri using in vitro mutagenesis and selection technique.ThesisItem Open Access GENETIC DIVERSITY OF INDIAN WILLOW (Salix tetrasperma Roxb.)(UHF,NAUNI, 2019-12) SHARMA, JAI PAL; SANKHYAN, H PABSTRACT The present investigation entitled “Genetic Diversity of Indian Willow (Salix tetrasperma Roxb.)” was carried out in the Department of Tree Improvement and Genetic Resources, Dr Y. S. Parmar University of Horticulture and Forestry, Nauni, Solan (H.P.) during 2015-2019 to assess the genetic diversity among various genotypes through morphological and molecular markers. Genotypes of the species from northern India were evaluated in common garden experiment. These were grouped into five latitudinal and four altitudinal ranges for morphological study and five populations for studying population diversity and structure. The analysis of variance revealed significant differences among genotypes, sites, latitudes and altitude ranges for all the traits. The plant height, basal diameter and biomass characters were recorded maximum for Suhanpur site.Number of nodes, internodal length and root length was recorded maximum for Devamanal, Rupnagar and Chamba sites, respectively. Udaipur and Bhunter sites recorded maximum foliage durations and sprouting days, respectively. Whereas, Devamanal site showed longest lamina and leaf length and Udaipur recorded maximum breadth of lamina. Dasua recorded maximum petiole length and Udaipur highest leaf area. Numbers of leaf teeth were observed maximum for Jammu site. The variability estimates exhibited high variability, high heritability along with genetic advance as percentage of mean. Linear regression analysis depicted that only characters foliage duration, leaf area and root length were under the influence of latitude. Whereas basal diameter, internodal length, number of nodes, maximum breadth of lamina, leaf area and all the biomass characters were affected by altitude. Principal component analysis extracted 82.85 percent of the total variance in five components. In genetic divergence studies 21 sites could be grouped into three clusters based on Ward’s method, separating sites of Punjab and Rajasthan from others. UPGMA dendrogram categorized all the genotypes used into five, four and three clusters with RAPD, ISSR and SSR markers, respectively. RAPD primers viz., OPC-02, OPL-08, OPL-09, OPA-01, DECA7, OPS-07, OPA-02; ISSR primers viz., UBC835, 814, ISSR1, ISSR7, 840, ISSR94, ISSR98, ISSR91 and SSR primers viz., SB100, ORPM28, ORPM21, WPMS18, WPMS03, ORPM488, ORPM496, PTR1 were found to be desirable due to higher polymorphic information content, marker index, resolving power, expected heterozygosity and are further suggested for genetic differentiation studies in willows. Higher observed heterozygosity than expected heterozygosity and negative fixation index through SSR markers confirmed outcrossing nature of the species. Phi-statistics of ISSR and F- Statistics of SSR revealed more variation among individuals within populations than among populations. The genetic differentiation (Fst) was significant and moderate with ISSR (0.138) and SSR (0.081) markers. Pairwise population of gene flow (Nm) values based on Fst values among populations showed the effect of geographic distance on migration of alleles. Pairwise population matrix of Nei genetic distance was obtained maximum between JK and HP1 populations. Bayesian clustering pattern through STRUCTURE software was supported by scatter plot diagram of principal coordinate analysis and dendrogram of molecular and morphological data.ThesisItem Open Access BIO-ECOLOGY AND MANAGEMENT OF INVASIVE LEAF-MINER, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) IN TOMATO(UHF,NAUNI, 2019-12) NEGI, SARSWATI; SHARMA, P LABSTRACT The present study entitled “Bio-ecology and management of the invasive leafminer, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) in tomato” was carried out under laboratory and field conditions at Department of Entomology, Dr. YS Parmar University of Horticulture and Forestry, Nauni, Solan (HP) India during 2015-19. Tuta absoluta has spread to almost all the tomato growing areas of the state with incidence ranging from 1-10 mines per leaf. Biological and population parameters of T. absoluta varied significantly with temperature and host plant. Tomato as host and temperature around 25-30 oC were the most ideal and suitable for its growth and development. Tuta absoluta required 500 degree-days with developmental thresholds of 7.5 oC to complete the development from egg to adult. Nesidiocoris tenuis was found to be an efficient predator with type II functional response to the pest. Based on LC50 values the order of toxicity of the tested insecticides to the pest was chlorantraniliprole > flubandiamide > novaluron > thiamethoxam > imidacloprid > indoxacarb > λ-cyhalothrin > quinalphos > spiromesifen. Under field conditions all the tested insecticides reduced T. absoluta population significantly over control with chlorantraniliprole to bethe most effective.ThesisItem Open Access ISOLATION AND CHARACTERIZATION OF CULTURABLE BACTERIAL ENDOPHYTES FROM ROOT TISSUES OF Rosemarinus officinalis AND DETERMINATION OF THEIR BIOACTIVITIES(UHF,NAUNI, 2019-11) MINAKSHI; CHAUHAN, ANJALIABSTRACT Medicinal plants are known to harbor distinctive and specific subset of bacterial endophytes that might be due to production of divergent bioactive compounds. There are many reports regarding production of bioactive compounds by these endophytic microorganisms but limited work has been done about plant growth promotion potential of endophytes associated with medicinal plants. Therefore, the present study was aimed to explore the diversity of plant growth promoting root endophytic bacterial communities associated with medicinal plant Rosemarinus officinalis and their characterization for plant growth promoting traits followed by their inoculation efficiency for plant growth promotion of rosemary. A total of ninety eight endorhizospheric bacteria were isolated fromroot samples ofRosemarinus officinaliscollected from two different sites ofKangra, Kullu, Solan and Sirmour valleys of Himachal Pradesh and were subjected to one step screening for phosphate solubilization, siderophore production and ability to fix nitrogen. After preliminary screening, a total of seventy nine isolates were selected for quantitative estimation of P-solubilzation, siderophore production and additional plant growth promoting traits viz. IAA, lytic enzymes production, production of secondary metabolites and antifungal activity. Forty two isolates exhibiting maximum PGP traits were subjected to study genetic diversity among them using 16S rDNA sequencing. In silico analysis grouped these isolates into nine major genera i.e. Bacillus, Pseudomonas being predominant while other were Pseudoxanthomonas, Lysobacter, Kosakonia, Ensifer, Beijerinckia, Oxynema and Serratia, respectively. Sole application of most efficient eleven isolates belonged to different genera (KA7, KU5, KA10, SI12, KU13, KU2, KU14, KA14, KA11, KA2 and KU21) resulted in significant increase in plant parameters of rosemary and soil physico-chemical properties under net house conditions. Isolates Bacillus subtilis strain KU21, Pseudomonas aeruginosa strain SI12, Cedecea lapagei strain KU14 produced most significant results under net house conditions and were further evaluated under field conditions. Field studies revealed that conjoint application of Bacillus subtilis strain KU21 and Pseudomonas aeruginosa strain SI12 increased plant physical, physiological parameters as well as soil physico-chemical properties over uninoculated control. Direct and indirect plant growth promotion by Bacillus subtilis strain KU21 and Pseudomonas aeruginosa strain SI12 was further confirmed by molecular and chromatographic analysis. Attempts have been made to understand the genetic basis of mineral phosphate solubilization in Bacillus subtilis strain KU21. Cloning and sequencing of gdh gene has paved the way for understanding molecular mechanisms involved in nutrient mobilization of essential nutrient (phosphorus) and its subsequent enhanced uptake by plants, as mediated by endophytes. Phytohormone production by Bacillus subtilis strain KU21 was also evaluated by TLC and HPLC analysis. Indirect plant growth promotion via biocontrol activities against tested fungal pathogens was best for Pseudomaonas aeruginosa SI12 hence characterized for extraction and identification of pyocyanin using HPLC. Overall, present study conclude that, exploring large pool of endophytic communities of medicinal plants appears to be a good strategy for selection of promising strains as PGPR and the potential application of these endophytes in agricultural traits could results in ameliorate plant production and health and in another way may lead to improve soil quality and fertility.ThesisItem Open Access STUDIES ON GENETIC DIVERSITY OF PHOSPHORUS SOLUBILIZING RHIZOBIA OF RAJMASH (PHASEOLUS VULGARIS L.)(UHF,NAUNI, 2019-11) GAUTAM, SWATI; KAUSHAL, RAJESHABSTRACT The present investigations were conducted to determine the genetic diversity of P-solubilizing rhizobia of rajmash by amplified ribosomal DNA restriction analysis (ARDRA). A total of 50 isolates associated with rajmash were authenticated as Rhizobium spp. on the basis of different authentication tests,viz.congo red test, bromothymol blue test, growth in Hofer’s alkaline broth, ketolactose medium and plant infection test. Out of 50 rhizobial isolates, all were P-solubilizers, sixteen were siderophore producers, nine were HCN producers, eight isolates showed antagonism against Rhizoctonia solani Kuhn and five against Colletotrichum lindemuthianum Sacc. and Mangus. The maximum P-solubilization (295.74 µg/ml) was recorded for isolate KS and maximum IAA production (83.19 µg/ml) was noted for isolate Har4. Maximum siderophoreproduction with zone size of 19mm was recorded with the isolate Shl7 on CAS medium. On the basis of banding pattern generated through ARDRA analysis using restriction enzymes Alu I and Msp I, 19 isolates from agro-climatic zone-II were grouped into seven major phylotypes at 49 per cent similarity level, 15 isolates from agroclimatic zone-III were segregated into five major phylotypes with 49 per cent similarity and 16 isolates from agro-climatic zone-IV were grouped into seven major phylotypes at 57 per cent similarity level. Seed bacterization with 10 selected rhizobia resulted in significant increase in shoot height, root length, plant biomass and number of nodules over uninoculated control. Among 10 sequenced rhizobia, 4 isolates viz. Hab3, BhrB, Tis2 and San1 were identified as Rhizobium nepotum, 3 isolates viz. KAF, Har1 and Kal3 as Rhizobium indigoferae, isolate KS as Rhizobium etli, isolate Har2 as Rhizobium acidisoli and isolate Shl3 as Rhizobium pusense. Seed bacterization with R. etli strain KS+70% P showed considerable effect on increase in shoot height (86 cm), root length (28.30 cm), number of nodules (55.07 no./plant) and available NPK over uninoculated control at flowering stage as well as harvest of the crop.ThesisItem Open Access SYNTHESIS AND CHARACTERIZATION OF SILVER NANOPARTICLES FROM POTENTIAL PROBIOTIC ISOLATES AND THEIR BIOTECHNOLOGICAL APPLICATIONS(UHF,NAUNI, 2019-11) SHARMA, SHAKSHI; SHARMA, NIVEDITAABSTRACT The present investigation was carried out to synthesize silver nanoparticles using five in-house potential probiotics i.e. Lactobacillus pentosus S6 (KU92122), Lactobacillus plantarum F22 (KT865223), Lactobacillus crustorum F11 (KT865221), Lactobacillus paraplantarum KM1 (KX671558) and Lactobacillus spicheri G2 (JX481912). All the five probiotic isolates were capable of synthesizing silver nanoparticles. The synthesized silver nanoparticles by five probiotic isolates were separately characterize by using UV-Vis spectroscopy, SEM, TEM and FTIR. The antimicrobial potential of these probiotics synthesized silver nanoparticles was assessed against different bacterial and fungal pathogens. Further the bacteriocin potential of five probiotic isolates was evaluated, out of five, three probiotic isolates i.e. L. pentosus S6, L. crustorum F11 and L. spicheri G2 were selected for the synthesis of silver nanoparticle on the basis of their wider antagonistic potential. These three in-house potential probiotic isolates were then subjected to their purification usingcolumn chromatography to obtain purified bacteriocin, which then was used in the synthesis of nanoparticles. The purity and molecular weight of purified bacteriocin of L. pentosusS6, L. crustorumF11 and L. spicheriG2 was determined by SDS–PAGE. The bacteriocin from three probiotic isolates showed their ability for extracellular biosynthesis of silver nanoparticles (Ba AgNP). The characterization of all the three Ba AgNP was carried separately using the following techniques i.e. UV-Vis spectroscopy, SEM, TEM and FTIR which confirms their formation. The antimicrobial efficacy of all the 3 bacteriocin synthesized silver nanoparticles was separately assessed against challenging antimicrobial pathogens. The application of bacteriocin synthesized silver nanoparticles (Ba AgNP) was observed in the preparation of bacteriocin capped silver nanoparticle coated paper, which was then used in the storage of different food items and increased the shelf life of food stored effectively. Further the effect of Ba AgNP was evaluated for biocontrol against P. aphanidermatum causing damping off in tomato under net house conditions. The nematicidal efficacy of the Ba AgNP was screened against root-knot nematode Meloidogyne incognita. The maximum mortality rate of 90 % was observed for bacteriocin synthesized silver nanoparticles i.e. F11 Ba AGNP. The in-vivo nematicidal efficacy of Ba AgNP was studied in tomato under net house conditions and the number of galls per plant was significantly reduced by Ba AgNPs treatments on tomato roots grown in soil amended with bacteriocin synthesized AgNPs. Thus these synthesized silver nanoparticles hold a great potential to be used in different biotechnological applications.