SYNTHESIS AND CHARACTERIZATION OF SILVER NANOPARTICLES FROM POTENTIAL PROBIOTIC ISOLATES AND THEIR BIOTECHNOLOGICAL APPLICATIONS
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Date
2019-11
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UHF,NAUNI
Abstract
ABSTRACT
The present investigation was carried out to synthesize silver nanoparticles using five in-house
potential probiotics i.e. Lactobacillus pentosus S6 (KU92122), Lactobacillus plantarum F22 (KT865223),
Lactobacillus crustorum F11 (KT865221), Lactobacillus paraplantarum KM1 (KX671558) and Lactobacillus
spicheri G2 (JX481912). All the five probiotic isolates were capable of synthesizing silver nanoparticles. The
synthesized silver nanoparticles by five probiotic isolates were separately characterize by using UV-Vis
spectroscopy, SEM, TEM and FTIR. The antimicrobial potential of these probiotics synthesized silver
nanoparticles was assessed against different bacterial and fungal pathogens. Further the bacteriocin potential of
five probiotic isolates was evaluated, out of five, three probiotic isolates i.e. L. pentosus S6, L. crustorum F11
and L. spicheri G2 were selected for the synthesis of silver nanoparticle on the basis of their wider antagonistic
potential. These three in-house potential probiotic isolates were then subjected to their purification usingcolumn
chromatography to obtain purified bacteriocin, which then was used in the synthesis of nanoparticles. The purity
and molecular weight of purified bacteriocin of L. pentosusS6, L. crustorumF11 and L. spicheriG2 was
determined by SDS–PAGE. The bacteriocin from three probiotic isolates showed their ability for extracellular
biosynthesis of silver nanoparticles (Ba AgNP). The characterization of all the three Ba AgNP was carried
separately using the following techniques i.e. UV-Vis spectroscopy, SEM, TEM and FTIR which confirms their
formation. The antimicrobial efficacy of all the 3 bacteriocin synthesized silver nanoparticles was separately
assessed against challenging antimicrobial pathogens. The application of bacteriocin synthesized silver
nanoparticles (Ba AgNP) was observed in the preparation of bacteriocin capped silver nanoparticle coated
paper, which was then used in the storage of different food items and increased the shelf life of food stored
effectively. Further the effect of Ba AgNP was evaluated for biocontrol against P. aphanidermatum causing
damping off in tomato under net house conditions. The nematicidal efficacy of the Ba AgNP was screened
against root-knot nematode Meloidogyne incognita. The maximum mortality rate of 90 % was observed for
bacteriocin synthesized silver nanoparticles i.e. F11 Ba AGNP. The in-vivo nematicidal efficacy of Ba AgNP
was studied in tomato under net house conditions and the number of galls per plant was significantly reduced by
Ba AgNPs treatments on tomato roots grown in soil amended with bacteriocin synthesized AgNPs. Thus these
synthesized silver nanoparticles hold a great potential to be used in different biotechnological applications.
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