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Dr. Y. S. Parmar University of Horticulture & Forestry, Solan

Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, was established on 1st December, 1985 with the objective to promote education, research and extension education in the fields of Horticulture, Forestry and allied disciplines. Late Dr. Yashwant Singh Parmar, the first Chief Minister and the architect of Himachal Pradesh perceived the importance of Horticulture and Forestry to develop and improve the State economy which led to the establishment of this University. Its history lies in erstwhile Himachal Agricultural College, Solan, established in 1962 and affiliated to the Panjab University. It became one of the campuses of Agriculture Complex of Himachal Pradesh University on its formation in 1970. Consequent upon the establishment of Himachal Pradesh Krishi Vishvavidyalaya in 1978, this campus became its Horticulture Complex and finally in 1985, assumed the status of a State University, being the only University in the country engaged exclusively in teaching, research and extension in Horticulture and Forestry. The University is located at Nauni in Solan District of Himachal Pradesh, 13 km from Solan on Solan-Rajgarh Road, at an elevation of 1300 metres above mean sea level. Solan town is situated on national highway (NH-22) and is well connected by train and bus services. The University has four constituent colleges, out of which, two are located at the main campus Nauni, one for horticulture and the other for forestry, having 9 and 7 departments, respectively. The third College i.e., College of Horticulture & Forestry is located at Neri in Hamirpur District on Nadaun-Hamirpur state highway, about 6 Km from Hamirpur town and is well connected with bus service. The college offers three Undergraduate Degree Programmes i.e. BSc (Hons.) Horticulture, BSc (Hons.) Forestry and B. Tech. Biotechnology and MSc degree programme in a few subjects. The fourth college i.e. College of Horticulture and Forestry, Thunag (Mandi) is located at Thunag District Mandi. This college offer BSc (Hons.) Horticulture and BSc (Hons.) Forestry degree programme. In addition, there are five Regional Research Stations, 12 Satellite Stations and five Krishi Vigyan Kendras (KVKs) situated in different zones of the State.

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20123
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Subject: Biotechnology and Molecular Biology × End date: 2012 × Start date: 2012 ×
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    ThesisItemOpen Access
    STUDIES ON PLANT GROWTH PROMOTING RHIZOBACTERIA ASSOCIATED WITH Podophyllum hexandrum
    (2012) SHARMA, PARUL; SHIRKOT, C.K.
    ABSTRACT Plant growth promoting rhizobacteria (PGPR) are known to influence plant growth by various direct or indirect mechanisms. Worldwide, there is a profound need to explore varied ecological niches for the presence of native microorganisms. Very limited information is available in respect of PGPR associated with medicinal plants such as Podophyllum hexandrum. Therefore, the aim of this study was to determine the plant growth promoting potential of plant growth promoting rhizobacteria (PGPR) isolated from Podophyllum hexandrum. Forty one bacterial isolates were selected (by replica plating technique) as the representative of the total plated population from the rhizosphere soil and rhizome/roots of the Podophyllum hexandrum from four sites of location Churdhar. All the bacterial isolates were able to grow simultaneously on nutrient agar, Pikovskaya’s, nitrogen free media and CAS media and selected for further screening for various plant growth promoting activities. Plant growth promotion assay was performed with tomato seedlings. Seed bacterization with bacterial strains increased the germination percent of tomato seed in filter paper assay as well as in growth chamber. The effect of seed treatment with bacterial isolates on per cent increase in root length (90%), shoot length (86.66%), shoot dry weight (334.46%) and plant biomass (240.32%) was found maximum with 2a1 inoculation and also with some other bacterial strains as compared to untreated control. From these results we conclude that the native strains with PGPR activity, can play an essential role in helping plants to establish and grow. Moreover, native strains described in this study could be employed to minimize the utilization of synthetic fungicides contributing to preservation of environment. Also screened isolates can be employed for growth promotion of Podophyllum hexandrum and other medicinal plants which face a major threat of extinction.
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    ThesisItemOpen Access
    STUDIES ON DEVELOPMENT OF PEACH GENIC-SSRs AND TRANSFERABILITY TO SOME ROSACEAE SPECIES
    (2012) SHILPA; KAUR, RAJINDER
    ABSTRACT In the present investigation EST – SSR markers were developed for peach and were further used for cross-transferability studies in some Rosaceae species viz., apricot, apple, rose and strawberry. EST sequences of Prunus persica available on NCBI website (www.ncbi.nlm.nih.gov/nucest) were screened for SSR motifs using an online tool, SSRIT (www.gramene.org/db/searches/SSRtool). 240 EST sequences were detected to contain SSRs, out of which 52 EST-SSRs were designed using PRIMER 3 software (www.frodo.wimit. edu/primer3/). Out of 52 EST-SSRs, 43 were custom synthesized. In the wet lab experimentation , DNA from fresh, young leaves of six genotypes of peach, four genotypes each of apricot, apple, rose and strawberry was isolated by CTAB method (Doyle and Doyle, 1987) for polymorphism and transferability studies. These 43 custom synthesized primers were used for polymorphism study in peach. Out of 43 primers, 38 primers showed amplification, out of which 20 were polymorphic, revealing 68% polymorphism in peach. These 20 polymorphic primers of peach were then further used to carry out transferability studies in apricot, apple, rose and strawberry. 50%, 95%, 95% and 45% transferability was observed in apricot, apple, rose and strawberry, respectively. Similarity matrices and dendrograms were generated using NTSys ver.2.02h and dissimilarity matrices and rooted trees were generated using DARwin 5 ver.5.0.155 for polymorphism data of all genotypes under study. All the 22 genotypes under study were grouped into two main clusters. Rooted tree of 22 genotypes revealed that ‘Lazy’ cultivar of apricot was singled out from rest of the cluster. Thus it is concluded from the present study that ESTs of peach produced high polymorphism in different Rosaceous species indicating their cross-transferability and use across different members of Rosaceous family.
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    ThesisItemOpen Access
    GENETIC FIDELITY OF MICROPROPAGATED PLANTS OF CARNATION (Dianthus caryophyllus L.)
    (2012) RAJNI, KUMARI; SHARMA, S.K.
    ABSTRACT The present investigation entitled “ Genetic fidelity of micropropagated plants of carnation (Dianthus caryophyllus L.)” was carried out to study the genetic similarity among the plants micropropagated through different technique of micropropagation viz. axillary buds, suspension and callus culture through RAPD markers. All types of shoots were multiplied on already standardized medium containing MS medium supplemented with 1.0 mg l-1 BA which was solidified with 1.0% agar in order to lessen the problem of vitrification. In vitro raised shoots were rooted in ½ strength MS medium supplemented with 2 mg l-1 IBA and 2 g l-1 activated charcoal and hardened successfully. Analysis of genomic DNA of mother plant and fifteen in vitro raised well acclimatized plants generates 35 Scorable bands, out of which 32, 28 and 29 bands were monomorphic among vegetativaly propagated (97.14%), suspension culture (80.00%) and callus culture (85.72%) derived plants respectively and 1, 7 and 5 polymorphic bands among vegetativaly propagated (2.86%), suspension culture (20.00%) and callus culture (14.28%) derived plants respectively. The presences of polymorphic fragments among regenerants indicate that genomic alteration occurred during long term culture of the cell. Similarity co-efficient value ranged from 0.97-1.00 for mother and axillary derived plants, 0.82-1.00 for mother and suspension culture derived plants and 0.85-1.00 for mother and callus derived plants. Hence, the regeneration system from organized meristem, such as shoot tip and axillary buds are considered to be most efficient method of micropropagation than others. Although plants derived from axillary buds showed the stability but not always genetically true to the type. Hence it is imperative to regularly check the genetic fidelity of micropropagated plants while using different techniques of micropropagation.