GENETIC FIDELITY OF MICROPROPAGATED PLANTS OF CARNATION (Dianthus caryophyllus L.)
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Date
2012
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ABSTRACT
The present investigation entitled “ Genetic fidelity of micropropagated plants of carnation
(Dianthus caryophyllus L.)” was carried out to study the genetic similarity among the plants
micropropagated through different technique of micropropagation viz. axillary buds, suspension and
callus culture through RAPD markers. All types of shoots were multiplied on already standardized
medium containing MS medium supplemented with 1.0 mg l-1 BA which was solidified with 1.0%
agar in order to lessen the problem of vitrification. In vitro raised shoots were rooted in ½ strength
MS medium supplemented with 2 mg l-1 IBA and 2 g l-1 activated charcoal and hardened successfully.
Analysis of genomic DNA of mother plant and fifteen in vitro raised well acclimatized plants
generates 35 Scorable bands, out of which 32, 28 and 29 bands were monomorphic among
vegetativaly propagated (97.14%), suspension culture (80.00%) and callus culture (85.72%) derived
plants respectively and 1, 7 and 5 polymorphic bands among vegetativaly propagated (2.86%),
suspension culture (20.00%) and callus culture (14.28%) derived plants respectively. The presences of
polymorphic fragments among regenerants indicate that genomic alteration occurred during long term
culture of the cell. Similarity co-efficient value ranged from 0.97-1.00 for mother and axillary derived
plants, 0.82-1.00 for mother and suspension culture derived plants and 0.85-1.00 for mother and callus
derived plants. Hence, the regeneration system from organized meristem, such as shoot tip and
axillary buds are considered to be most efficient method of micropropagation than others. Although
plants derived from axillary buds showed the stability but not always genetically true to the type.
Hence it is imperative to regularly check the genetic fidelity of micropropagated plants while using
different techniques of micropropagation.