GENETIC FIDELITY OF MICROPROPAGATED PLANTS OF CARNATION (Dianthus caryophyllus L.)

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dc.contributor.advisorSHARMA, S.K.
dc.contributor.authorRAJNI, KUMARI
dc.date.accessioned2016-12-26T12:26:46Z
dc.date.available2016-12-26T12:26:46Z
dc.date.issued2012
dc.description.abstractABSTRACT The present investigation entitled “ Genetic fidelity of micropropagated plants of carnation (Dianthus caryophyllus L.)” was carried out to study the genetic similarity among the plants micropropagated through different technique of micropropagation viz. axillary buds, suspension and callus culture through RAPD markers. All types of shoots were multiplied on already standardized medium containing MS medium supplemented with 1.0 mg l-1 BA which was solidified with 1.0% agar in order to lessen the problem of vitrification. In vitro raised shoots were rooted in ½ strength MS medium supplemented with 2 mg l-1 IBA and 2 g l-1 activated charcoal and hardened successfully. Analysis of genomic DNA of mother plant and fifteen in vitro raised well acclimatized plants generates 35 Scorable bands, out of which 32, 28 and 29 bands were monomorphic among vegetativaly propagated (97.14%), suspension culture (80.00%) and callus culture (85.72%) derived plants respectively and 1, 7 and 5 polymorphic bands among vegetativaly propagated (2.86%), suspension culture (20.00%) and callus culture (14.28%) derived plants respectively. The presences of polymorphic fragments among regenerants indicate that genomic alteration occurred during long term culture of the cell. Similarity co-efficient value ranged from 0.97-1.00 for mother and axillary derived plants, 0.82-1.00 for mother and suspension culture derived plants and 0.85-1.00 for mother and callus derived plants. Hence, the regeneration system from organized meristem, such as shoot tip and axillary buds are considered to be most efficient method of micropropagation than others. Although plants derived from axillary buds showed the stability but not always genetically true to the type. Hence it is imperative to regularly check the genetic fidelity of micropropagated plants while using different techniques of micropropagation.en_US
dc.identifier.other47814
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/92857
dc.language.isoenen_US
dc.subBiotechnology and Molecular Biology
dc.these.typeM.Sc
dc.titleGENETIC FIDELITY OF MICROPROPAGATED PLANTS OF CARNATION (Dianthus caryophyllus L.)en_US
dc.typeThesisen_US
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