STUDIES ON PLANT GROWTH PROMOTING RHIZOBACTERIA ASSOCIATED WITH Podophyllum hexandrum
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Date
2012
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Abstract
ABSTRACT
Plant growth promoting rhizobacteria (PGPR) are known to influence plant growth by various direct or
indirect mechanisms. Worldwide, there is a profound need to explore varied ecological niches for the presence
of native microorganisms. Very limited information is available in respect of PGPR associated with medicinal
plants such as Podophyllum hexandrum. Therefore, the aim of this study was to determine the plant growth
promoting potential of plant growth promoting rhizobacteria (PGPR) isolated from Podophyllum hexandrum.
Forty one bacterial isolates were selected (by replica plating technique) as the representative of the total plated
population from the rhizosphere soil and rhizome/roots of the Podophyllum hexandrum from four sites of
location Churdhar. All the bacterial isolates were able to grow simultaneously on nutrient agar, Pikovskaya’s,
nitrogen free media and CAS media and selected for further screening for various plant growth promoting
activities. Plant growth promotion assay was performed with tomato seedlings. Seed bacterization with bacterial
strains increased the germination percent of tomato seed in filter paper assay as well as in growth chamber. The
effect of seed treatment with bacterial isolates on per cent increase in root length (90%), shoot length (86.66%),
shoot dry weight (334.46%) and plant biomass (240.32%) was found maximum with 2a1 inoculation and also
with some other bacterial strains as compared to untreated control. From these results we conclude that the
native strains with PGPR activity, can play an essential role in helping plants to establish and grow. Moreover,
native strains described in this study could be employed to minimize the utilization of synthetic fungicides
contributing to preservation of environment. Also screened isolates can be employed for growth promotion of
Podophyllum hexandrum and other medicinal plants which face a major threat of extinction.
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Keywords
planting, bacteria, biological development, productivity, vegetative propagation, inorganic acid salts, growth promoters, fungi, sowing, enzymes, rhizobacteria (PGPR) , Podophyllum hexandrum