Thumbnail Image

University of Agricultural Sciences, Bengaluru

University of Agricultural Sciences Bangalore, a premier institution of agricultural education and research in the country, began as a small agricultural research farm in 1899 on 30 acres of land donated by Her Excellency Maharani Kempa Nanjammanni Vani Vilasa Sannidhiyavaru, the Regent of Mysore and appointed Dr. Lehmann, German Scientist to initiate research on soil crop response with a Laboratory in the Directorate of Agriculture. Later under the initiative of the Dewan of Mysore Sir M. Vishweshwaraiah, the Mysore Agriculture Residential School was established in 1913 at Hebbal which offered Licentiate in Agriculture and later offered a diploma programme in agriculture during 1920. The School was upgraded to Agriculture Collegein 1946 which offered four year degree programs in Agriculture. The Government of Mysore headed by Sri. S. Nijalingappa, the then Chief Minister, established the University of Agricultural Sciences on the pattern of Land Grant College system of USA and the University of Agricultural Sciences Act No. 22 was passed in Legislative Assembly in 1963. Dr. Zakir Hussain, the Vice President of India inaugurated the University on 21st August 1964.

Browse

2006 - 200934
Reset filters
Subject: Plant Biotechnology × End date: 2009 × Start date: 2006 ×
Reset filters

Search Results

Now showing 1 - 9 of 34
  • Thumbnail Image
    ThesisItemOpen Access
    PURIFICATION AND EVALUATION OF LOW MOLECULAR WEIGHT SUBABUL TRYPSIN INHIBITOR AS POTENTIAL INSECTICIDAL PROTEIN
    (UNIVERSITY OF AGRICULTURAL SCIENCES, BANGALORE, 2006-08-19) MADHUKARA., K. M; THEERTHA PRASAD, D.
    No Abstract
  • Thumbnail Image
    ThesisItemOpen Access
    MOLECULAR CHARACTERIZATION OP NUCLEAR POLYHEDROSIS VIRUS INFECTING Bombyx mori L. (BmNPV) AND PREVENTION OF ITS MULTIPLICATION BY USING HERBAL EXTRACTS
    (UNIVERSITY OF AGRICULTURAL SCIENCES, BANGALORE, 2006-07-14) CHANDRAMOHAN, J.; Anitha, Peter
    No Abstract
  • Thumbnail Image
    ThesisItemOpen Access
    Molecular fingerprinting of french bean accessions by DNA markers
    (UNIVERSITY OF AGRICULTURAL SCIENCES, BANGALORE, 2006-07-09) DIVYA, P.S.; Ananthanarayanan, T.V
    No Abstract
  • Thumbnail Image
    ThesisItemOpen Access
  • Thumbnail Image
    ThesisItemOpen Access
    GENETIC DIVERSITY STUDIES AMONG FIELD BEAN (Lablab purpureus L.) GENOTYPES USING AFLP MARKERS
    (UNIVERSITY OF AGRICULTURAL SCIENCES, BANGALORE, 2006-01-19) PRAKASH, G. PATIL; ASHOK, T. H.
    No Abstract
  • Thumbnail Image
    ThesisItemOpen Access
    MOLECULAR CHARACTERIZATION OF YEAST STRAINS FOR EFFICIENT ETHANOL PRODUCTION USING SSR MARKERS
    (University of Agricultural Sciences, Bangalore, 2009-07-15) AYEESHA, MUNAWERY; HARINIKUMAR, K.M.
    The study was carried out to isolate yeast strains from their natural habitat and to screen them for ethanol tolerance. A total of 45 yeast strains were isolated from sugar rich sources. Twelve were identified as Saccharomyces spp. Based on colony type and cell morphological characters such as cell shape and budding characters. Saccharomyces spp. were screened for the ability to tolerate different ethanol concentrations from 5-15% growth in different ethanol concentrations varied from one isolate to another. Yeast isolates showed tolerance level from 5-15%. The best strain had 15% ethanol tolerance strain YDE, which showed high tolerance to ethanol and YMS with low tolerance were mutated by UV radiations with time intervals of 1, 3 and 5 mins and through chemical method using Acridine orange and Ethidium bromide at concentrations of 0.25, 0.50. 0.75 and 1.00 g/ml and were subjected to screening under same ethanol concentrations from 5-15%. Mutated isolates showed decreased growth and tolerance under high ethanol stress compared to their original isolates. SSR profiling was employed to characterize yeast isolates. Eleven Saccharomyces spp. were selected and were subjected for SSR analysis using 10 yeast specific SSR primers selected from public domain and constructed by chromos Biotech Company. Showed polymorphism among the isolates. Dendrogram was constructed according to unweighted pair group arithmetic means using STATISTICA software. Cluster analysis revealed major two groups, two isolates formed one group and other nine isolates formed other group. There was no correlation between SSR profiling and physiological screening of yeast isolates for ethanol tolerance.
  • Thumbnail Image
    ThesisItemOpen Access
    TRANSFORMATION OF POMEGRANATE (Punica granatum L.) BY AMP (ANTIMICROBIAL PEPTIDE) GENE TO CONFER RESISTANCE AGAINST BACTERIAL BLIGHT OF POMEGRANATE
    (University of Agricultural Sciences, Bangalore, 2009-07-15) NUNGSHILEPDEN; SUKHADA, MOHANDAS
    Pomegranate is a popular fruit crop and is of considerable economic importance. The bacterial blight of pomegranate is becoming a serious problem in major pomegranate growing area in India. All conventional ways and means of controlling this disease have failed. In pomegranate, existing protocols have shown slow response by regeneration and less work has been reported on transformation. Hence, in the present study, a systematic investigation was carried to standardize an efficient in vitro regeneration protocol from different explants of pomegranate cv. Bhagwa, find out the best treatment for faster regeneration and transforming it with AMP gene. As a result, we could able to achieve a reliable and efficient regeneration protocol and standardize an efficient transformation protocol. Agrobacterium tumefaciens carrying gene pCAMBIA construct with the constitutive CaMV35S promoter, AMP gene terminator and nptII selectable marker (Kanamycin resistance), was used for transformation of explants. Putative transformants were identified on selection medium containing kanamycin at different concentration. Transgene insertion and expression at various levels were confirmed using PCR. Out of four putative transformants analyzed, three transgenic plants showed PCR +ve with AMP gene specific primer and further screening is going on using different molecular techniques.
  • Thumbnail Image
    ThesisItemOpen Access
    MOLECULAR FINGERPRINTING OF OKRA ACCESSIONS BY DNA MARKERS
    (University of Agricultural Sciences, Bangalore, 2009-07-15) PRASHANTH KUMAR, G. M.; ANANTHANARAYANAN, T. V.
    The molecular analysis of genetic diversity and relatedness assumes considerable significance in evolving appropriate strategies for crop breeding. These investigations were focused on the assessment of the genetic diversity in okra genotypes using DNA markers. In the present study, eighty four accessions were studied to know the genetic similarity using RAPD markers. Out of 60 primers screened, 8 gave good amplification and polymorphism. Out of 8 primers, OPF 10 gave the highest discriminative power (%) i.e. 10.20. OPG 10 gave least polymorphism (50%) among 8 primers. Among the eighty-four accessions, eight random primers generated 17 scorable RAPD loci of which 16 RAPD loci were polymorphic (94.11%). The average number of polymorphic bands per primer was 2. endrogram was constructed based on the pooled data using the STATISTICA software. The genetic dissimilarity value in the distance matrix ranged from 0 to 15% suggesting a narrow genetic base within the okra genotypes. Cluster analysis based on 17 number of RAPD polymorphic bands revealed that 84 genotypes were clustered at a linkage distance of 156 units in the dendrogram with IC 282288 and IC-33315 spanning the extremes. The data generated can be used to improve the agronomically important traits of okra. Knowledge on the genetic variability in okra accessions increases the efficiency of germplasm conservation and enables precision breeding for crop improvement. Key words: RAPD, Abelmoschus esculentus L, genetic diversity, DNA polymorphism
  • Thumbnail Image
    ThesisItemOpen Access
    ISOLATION AND MOLECULAR CHARACTERIZATION OF Bacillus megaterium AND THE RESPONSE OF AEROBIC RICE TO THE ISOLATES
    (University of Agricultural Sciences, Bangalore, 2009-07-15) REMYA, G. PILLAI; SURESH, C. K.
    The investigation was carried out to study the molecular characterization of Bacillus megaterium isolated from soils of the ten different agroclimatic zones of Karnataka. A pot culture experiment was carried out to find out the influence of Bacillus megaterium on aerobic rice under greenhouse conditions. Aerobic rice plants inoculated with Bacillus megaterium isolates manifested increase in plant height, number of leaves, number of tillers, biomass and grain weight compared to uninoculated plants. Among the ten isolates inoculated, the Bacillus megaterium isolates form zone 7 (Southern transition zone) recorded significantly high values in almost all growth parameters chosen for the study. Simultaneously biochemical parameters of the aerobic rice inoculated with 10 isolates of Bacillus megaterium was studied. In the inoculated plants the biochemical and chemical parameters like chlorophyll content, nitrogen, phosphorous, and total sugars was higher as compared to uninoculated plants. The diversity of these isolates was characterized by randomly amplified polymorphic DNA (RAPD) marker. RAPD analysis revealed a total of 47 bands, out of which 40 bands were found to be polymorphic. The RAPD marker analysis clearly depicted that all the ten Bacillus megaterium isolates formed two major clusters. Among the two major groups, isolates from zone 3 and zone 4 formed the first group while those from zone 1, zone 2, zone 5, zone 6, zone 7, zone 8, zone 9 and zone 10 formed the second group. The RAPD banding pattern of these isolates could easily distinguish the isolates of different zones. Variation in these isolates was also reflected on the growth and development of aerobic rice plants as discussed above.