MOLECULAR CHARACTERIZATION OF YEAST STRAINS FOR EFFICIENT ETHANOL PRODUCTION USING SSR MARKERS
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Date
2009-07-15
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University of Agricultural Sciences, Bangalore
Abstract
The study was carried out to isolate yeast strains from their natural habitat and to
screen them for ethanol tolerance. A total of 45 yeast strains were isolated from sugar
rich sources. Twelve were identified as Saccharomyces spp. Based on colony type and
cell morphological characters such as cell shape and budding characters. Saccharomyces
spp. were screened for the ability to tolerate different ethanol concentrations from 5-15%
growth in different ethanol concentrations varied from one isolate to another. Yeast
isolates showed tolerance level from 5-15%. The best strain had 15% ethanol tolerance
strain YDE, which showed high tolerance to ethanol and YMS with low tolerance were
mutated by UV radiations with time intervals of 1, 3 and 5 mins and through chemical
method using Acridine orange and Ethidium bromide at concentrations of 0.25, 0.50.
0.75 and 1.00 g/ml and were subjected to screening under same ethanol concentrations
from 5-15%. Mutated isolates showed decreased growth and tolerance under high ethanol
stress compared to their original isolates. SSR profiling was employed to characterize
yeast isolates. Eleven Saccharomyces spp. were selected and were subjected for SSR
analysis using 10 yeast specific SSR primers selected from public domain and
constructed by chromos Biotech Company. Showed polymorphism among the isolates.
Dendrogram was constructed according to unweighted pair group arithmetic means using
STATISTICA software. Cluster analysis revealed major two groups, two isolates formed
one group and other nine isolates formed other group. There was no correlation between
SSR profiling and physiological screening of yeast isolates for ethanol tolerance.
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