MOLECULAR CHARACTERIZATION OF YEAST STRAINS FOR EFFICIENT ETHANOL PRODUCTION USING SSR MARKERS
| dc.contributor.advisor | HARINIKUMAR, K.M. | |
| dc.contributor.author | AYEESHA, MUNAWERY | |
| dc.date.accessioned | 2017-06-30T06:44:51Z | |
| dc.date.available | 2017-06-30T06:44:51Z | |
| dc.date.issued | 2009-07-15 | |
| dc.description.abstract | The study was carried out to isolate yeast strains from their natural habitat and to screen them for ethanol tolerance. A total of 45 yeast strains were isolated from sugar rich sources. Twelve were identified as Saccharomyces spp. Based on colony type and cell morphological characters such as cell shape and budding characters. Saccharomyces spp. were screened for the ability to tolerate different ethanol concentrations from 5-15% growth in different ethanol concentrations varied from one isolate to another. Yeast isolates showed tolerance level from 5-15%. The best strain had 15% ethanol tolerance strain YDE, which showed high tolerance to ethanol and YMS with low tolerance were mutated by UV radiations with time intervals of 1, 3 and 5 mins and through chemical method using Acridine orange and Ethidium bromide at concentrations of 0.25, 0.50. 0.75 and 1.00 g/ml and were subjected to screening under same ethanol concentrations from 5-15%. Mutated isolates showed decreased growth and tolerance under high ethanol stress compared to their original isolates. SSR profiling was employed to characterize yeast isolates. Eleven Saccharomyces spp. were selected and were subjected for SSR analysis using 10 yeast specific SSR primers selected from public domain and constructed by chromos Biotech Company. Showed polymorphism among the isolates. Dendrogram was constructed according to unweighted pair group arithmetic means using STATISTICA software. Cluster analysis revealed major two groups, two isolates formed one group and other nine isolates formed other group. There was no correlation between SSR profiling and physiological screening of yeast isolates for ethanol tolerance. | en_US |
| dc.identifier.other | Th-9542 | |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810023842 | |
| dc.language.iso | en | en_US |
| dc.publisher | University of Agricultural Sciences, Bangalore | en_US |
| dc.sub | Plant Biotechnology | en_US |
| dc.subject | null | en_US |
| dc.theme | MOLECULAR CHARACTERIZATION OF YEAST STRAINS | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | MOLECULAR CHARACTERIZATION OF YEAST STRAINS FOR EFFICIENT ETHANOL PRODUCTION USING SSR MARKERS | en_US |
| dc.type | Thesis | en_US |