MOLECULAR FINGERPRINTING OF OKRA ACCESSIONS BY DNA MARKERS
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Date
2009-07-15
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University of Agricultural Sciences, Bangalore
Abstract
The molecular analysis of genetic diversity and relatedness assumes considerable significance in evolving appropriate strategies for
crop breeding. These investigations were focused on the assessment of the genetic diversity in okra genotypes using DNA markers. In the present study, eighty four accessions were studied to know the genetic similarity using RAPD markers. Out of 60 primers screened, 8 gave good amplification and polymorphism. Out of 8 primers, OPF 10 gave the highest discriminative power (%) i.e. 10.20. OPG 10 gave least polymorphism (50%) among 8 primers. Among the eighty-four accessions, eight random primers generated 17 scorable RAPD loci of which 16 RAPD loci were polymorphic (94.11%). The average number of polymorphic bands per primer was 2. endrogram was constructed based on the pooled data using the STATISTICA software. The genetic dissimilarity value in the distance matrix ranged from 0 to 15% suggesting a narrow genetic base within the okra genotypes. Cluster analysis based on 17 number of RAPD polymorphic bands revealed that 84 genotypes were clustered at a linkage distance of 156 units in the dendrogram with IC 282288 and IC-33315 spanning the extremes. The data generated can be used to improve the agronomically important traits of okra. Knowledge on the genetic variability in okra accessions increases the efficiency of germplasm conservation and enables precision breeding for crop improvement. Key words: RAPD, Abelmoschus esculentus L, genetic diversity, DNA polymorphism
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