ISOLATION AND MOLECULAR CHARACTERIZATION OF Bacillus megaterium AND THE RESPONSE OF AEROBIC RICE TO THE ISOLATES
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Date
2009-07-15
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University of Agricultural Sciences, Bangalore
Abstract
The investigation was carried out to study the molecular
characterization of Bacillus megaterium isolated from soils of the ten
different agroclimatic zones of Karnataka. A pot culture experiment was
carried out to find out the influence of Bacillus megaterium on aerobic
rice under greenhouse conditions. Aerobic rice plants inoculated with
Bacillus megaterium isolates manifested increase in plant height, number
of leaves, number of tillers, biomass and grain weight compared to
uninoculated plants. Among the ten isolates inoculated, the Bacillus
megaterium isolates form zone 7 (Southern transition zone) recorded
significantly high values in almost all growth parameters chosen for the
study. Simultaneously biochemical parameters of the aerobic rice
inoculated with 10 isolates of Bacillus megaterium was studied. In the
inoculated plants the biochemical and chemical parameters like
chlorophyll content, nitrogen, phosphorous, and total sugars was higher
as compared to uninoculated plants. The diversity of these isolates was
characterized by randomly amplified polymorphic DNA (RAPD) marker.
RAPD analysis revealed a total of 47 bands, out of which 40 bands were
found to be polymorphic. The RAPD marker analysis clearly depicted
that all the ten Bacillus megaterium isolates formed two major clusters.
Among the two major groups, isolates from zone 3 and zone 4 formed the
first group while those from zone 1, zone 2, zone 5, zone 6, zone 7, zone
8, zone 9 and zone 10 formed the second group. The RAPD banding
pattern of these isolates could easily distinguish the isolates of different
zones. Variation in these isolates was also reflected on the growth and
development of aerobic rice plants as discussed above.
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