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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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2014 - 201611
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Subject: Molecular Biology and Biotechnology × Start date: 2014 × Thesis Type: M.Sc ×
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Now showing 1 - 9 of 11
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    ThesisItemOpen Access
    Micronutrient and SSR marker analysis of pearl millet [Pennisetum glaucam (L.) R. Br.] inbred lines
    (CCSHAU, 2015) Sangwan, Sonali; Yashveer, Shikha
    A set of thirty-six pearl millet inbred lines was used to study genetic variability and correlation analysis for seven morphological characters namely days to 50% flowering, plant height (cm), ear length (cm), ear diameter (cm), 1000 grain weight (g), grain yield per plant (g) and dry fodder yield per plant (g) along with micronutrient (Fe and Zn) status. The pearl millet inbred lines were evaluated in randomized block design with two replications in two environments during 2014. Analysis of variance for all the characters under study showed significant differences indicating the presence of genetic variability among the inbreds. The estimates of variability parameter for grain yield, contributing traits and Fe and Zn content revealed the high PCV value as compared to GCV value that suggests the role of environment in the expression of all these characters. An additive gene action was indicated for dry fodder yield per plant, grain yield per plant, Fe and Zn content by high to moderate heritability and genetic advance as per cent of mean values. Correlation analysis showed highly significant correlation between grain Fe and Zn content which signifies simultaneous improvement in the two traits. Grain yield per plant showed no significant negative correlation with Fe and Zn that suggests improvement in nutrient value without sacrificing yield. A set of 30 SSR primers was also used for molecular diversity assessment in 36 pearl millet inbred lines. Mean allele per locus and PIC obtained was 10.5 and 0.796, respectively. Cluster analysis distributed these genotypes into 9 clusters where 4 genotypes failed to fall in any of these clusters.
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    ThesisItemOpen Access
    Plant regeneration in cultivated and wild species of cluster bean (Cyamopsis spp.)
    (CCSHAU, 2016) Puja Rani; Yadav, Neelam R.
    Cluster bean (Camopsis tetragonoloba L. Taub) commonly known as guar, is a hardy crop and considered as green gold due to galactomannan content.The present study was undertaken to observe the response of various explants viz., immature cotyledon, immature embryo, Cotyledonary node and hypocotyl of three genotypes HG2-20, HG 563 and C. serrataShinz.on different growth regulators and concentrations. The Cultured cotyledonary node and hypocotyl explants (15 days old) were taken from in vitro grown seedlings andimmature cotyledon, immature embryo axes explants taken fron pods. A total of 13, MS modified media supplemented with different concentrations of growth regulators TDZ, BAP, Kin and NAA were used for in vitro culture of explants. MS medium supplemented with TDZ alone and TDZ in combination with NAA was the most responsive medium for shoot regenerationin all three genotypes.Among the various explants, Cotyledonary node explants werefound to be the most responsive.Hypocotyl explants did not show shoot formation in all three genotypes. Both immature cotyledon and immature embryo axes explants showed good response for shoot regeneration on MS medium supplemented with TDZ (5 μM) with NAA (2 mg/l).The best shoot regeneration response (99.66%) from cotyledonary node explants was observed on MS medium containing TDZ (5μM) in C. tetragonoloba cv. HG 2-20. Maximum shoots per explant (Eight) was observed from embryo axes explants of C. serrata on MS medium supplemented with TDZ (5 μM). A total of Four MS modified media were used for rooting. None of media induced roots in all three genotypes.
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    ThesisItemOpen Access
    Identification of hybrids and their parents through SSR profiling in maize [zea mays (L.)]
    (CCSHAU, 2016) Dinesh; Kharb, Pushpa
    Zea mays (L.), commonly known as maize or corn, is a diploid grain crop with 20 chromosomes (n=10) belonging to the family Poaceae. It is an important staple food in many countries and is also used in animal feed and has many industrial applications. The introduction of new hybrid seeds that can survive low winter conditions, off-season diseases and pests with high productivity has made maize a profitable alternative for small farmers. Higher genetic purity is an essential pre–requisite for commercialization of any hybrid because mixing of hybrids can decrease the production rate. So, clear cut identification is essential for protection and prevention of unauthorized commercial usage. The SSR markers are preferred molecular markers for genetic purity identification in many crops due to their high efficiency, co-dominance, reproducibility, simplicity and accessibility to laboratories. Total seven maize hybrids along with their ten parents were used in the study for identification of maize hybrids and their parents. Genomic DNA of maize hybrids (HM 5, HM 8, HM 9, HM 10, HM 11, HM 12, HM 13) and their respective parents (Hki 1344, Hki 1348-6-2, Hki 1105, Hki 161, Hki 1128, Hki 193-2, Hki 163, Hki 1378, Hki 488 IPG, Hki 193-1) were isolated using CTAB method. A total of 45 SSR primer pairs were used for amplification of the isolated genomic DNA. Gel electrophoresis was carried out for the separation and detection of the amplified products. Out of 45 SSR primer pairs used, 3 SSR primers could distinguish the different hybrids and their parents. The study will be very useful in detecting unwanted seed mixed with hybrid seed.
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    ThesisItemOpen Access
    SSR marker based identification of maize (Zea mays L.) hybrids
    (CCSHAU, 2016) Chaudhary, Shalu; Kharb, Pushpa
    Zea mays, commonly called maize is a grain crop (n = 10) belonging to the family Poaceae. It is an important staple food in many countries and is also used in animal feed and has many industrial applications. Genetic diversity of maize plays a key role in maize breeding. The introduction of new hybrid seeds that can survive low winter conditions, off-season diseases and pests with high productivity has made maize a profitable alternative for small farmers. Higher genetic purity is an essential pre–requisite for commercialization of any hybrid because mixing of hybrids can decrease the production rate. So, clear cut identification is essential for protection and prevention of unauthorized commercial usage. The SSR markers are preferred molecular markers for genetic purity identification in many crops due to their high efficiency, co-dominance, reproducibility, simplicity and accessibility to laboratories so, in this study a total of 75 SSR primer pairs were used for identification of maize hybrids and their parents. Genomic DNA of maize hybrids and their parents (HKI193-1×HKI163-HQPM1, HKI1105×HKI323-HM4, HKI1352×HKI1344-HM2, HKI193-2 ×HKI161-HQPM1, HKI163×HKI161-HQPM5, and HKI193-1 × HKI16-HQPM7) were isolated using CTAB method. Gel electrophoresis was carried out for the separation and detection of the amplified products. Out of 75 SSR primer pairs used, 8 SSR primers could distinguish the different hybrids from their parents. The SSR data was used to determine genetic relationship among these genotypes by creating dendrogram. Cluster analysis distributed these hybrids and their parents genotypes into two major clusters and further, in two sub clusters. The number of allele per locus varied from was 1-3 and PIC value obtained were 0.402 for 2 alleles and 0.523 for 3 alleles. The study will be very useful in detecting unwanted seed mixed with hybrid seed.
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    ThesisItemOpen Access
    Marker assisted selection for fusarium wilt and ascochyta blight resistance in chickpea (Cicer arietinum L.)
    (CCSHAU, 2016) Sandeep Kumar; Kharb, Pushpa
    The present investigation on marker assisted selection for resistance to Fusarium wilt and Ascochyta blight in chickpea was conducted with the objectives to screen the F3 genotypes of two crosses of chickpea for QTLs conferring resistance to Fusarium wilt and Ascochyta blight and to phenotype F 3-4 genotypes having QTLs resistant for Fusarium wilt under artificial conditions and Ascochyta blight under natural conditions. For selection of genotypes carrying QTL(s) for disease resistance, 96 genotypes of F3 generation along with parents HC 1 and H 91-36, and 68 genotypes of F 3 generation along with their parents GNG 663 and H 04-99 were used for Fusarium wilt and Ascochyta blight. 20 SSR primers linked to QTLs for resistance to Fusarium wilt (8 SSR primers) and Ascochyta blight (12 SSR Primers) were used. These 20 SSR were used to discriminate the parental genotypes in which for wilt 3 SSR primers and for blight 4 SSR primers showed polymorphism in cross HC 1 × H 91-36 while for wilt 6 SSR primers and for blight 8 SSR primers showed polymorphism in cross GNG 663 × H 04-99. By using the primers showed polymorphism for both Fusarium wilt and Ascochyta blight, 52 genotypes for Fusarium wilt and 64 genotypes for Ascochyta blight from cross HC 1 × H 91-36 were found having resistant QTLs. 50 genotypes for Fusarium wilt and 48 genotypes for Ascochyta blight from cross GNG 663 × H 04-99 were found having resistant QTLs. In cross HC 1 × H 91-36, 14 genotypes for Fusarium wilt and 20 genotypes for Ascochyta blight highly resistant while in cross GNG 663 × H 04 99, 20 genotype for Fusarium wilt and 17 genotypes for Ascochyta blight highly resistant were found. In collective investigation, 3 genotypes highly resistant and 6 genotypes resistant for both Fusarium wilt and Ascochyta blight were found in the cross HC 1 x H 91-36 and 6 genotypes highly resistant and 2 genotype resistant for both Fusarium wilt Ascochyta blight were found in the cross GNG 663 x H 04-99.
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    ThesisItemOpen Access
    Physio-morphological and molecular evaluation of Kharchia65 (Salt tolerant) x WH1105 (high yielding) F2 population in wheat (Triticum aestivum L. em Thell.)
    (CCSHAU, 2016) Dang Nguyen Luu Vi Vy; Shikha Yashveer
    Experiments were conducted to validate markers (Nax1 and Nax2) for salinity tolerance and to study Physio-morphological and molecular evaluation of F 2 population in wheat (Triticum aestivum L. em Thell.). Net house evaluation data showed enormous variation among Kharchia65 x WH1105 F 2 plants including plant height (cm), No. of tillers per plant, ear length (cm), No. of grains /ear, No. of spikelets/spike, 1000 grain weight (g), No. of grains per plant, biological yield per plant (g), harvest index (%), main spike weight (g) and grain yield per plant under salt stress condition. All the 208 F 2 plants were checked for Nax loci. Out of 208 Kharchia65 x WH1105 F 2 plants, 62 plants had both Nax1 and Nax2 loci. All the 62 F 2 plants were divided into seven major clusters interspersed between the two parental lines with the similarity coefficient of 0.55 Out of these 62 F 2 plants those having higher yield could be further backcrossed with the parent WH1105 to select salt tolerant wheat lines The information generated from this study can be used further in the improvement of wheat through various breeding programmes.
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    ThesisItemOpen Access
    Phenotyping and genotyping for salt tolerance of KHARCHIA65 X HD2285 F2 population in wheat (Triticum aestivum L.em.Thell)
    (CCSHAU, 2014) Suman; Shikha Yashveer
    Experiments were conducted to validate markers (Nax1 and Nax2) for salinity tolerance and phenotyping and genotyping of F 2 Population of cross Kharchia65x HD2285. Net house evaluation data showed enormous variation among Kharchia65x HD2285F 2 plants including plant height (cm), No. of tillers per plant, ear length (cm), No. of grains /ear, No. of spikelets/spike, No. of grains/spikelet, grain yield per plant, 1000 grain weight (g), biological yield/plant (g), harvest index (%) and germination percentage under salt stress condition. ). 90 plants were selected with high yield per plant and their tolerance to salt stress but only 20 plants were found to be Nax2 positive. All the 20 F 2 plants clustered in two major groups at the similarity coefficient of 0.43. Major group 1 consisted of parent HD2285 while major group II had Kharchia65 and all F 2 plants. This is the probably the first report where Nax2 has been validated in Kharchia65 and it clearly indicates that a linked marker like Nax2 could provide a valuable tool for breeding wheat with enhanced tolerance to salinity conditions.
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    ThesisItemOpen Access
    Phenotyping and microsatellite marker analysis for yellow rust in WH711 × WH542 F2 population of wheat (Triticum aestivum L.em.Thell)
    (CCSHAU, 2014) Vashisth, Medhavi; Dhillon, Santosh
    Stripe rust (yellow rust), caused by Puccinia striiformis Westend. f. sp. tritici Eriks., is one of the most devastating diseases of wheat throughout the world. Marker assisted breeding is the most economical and environmentally acceptable method to control stripe rust. The present investigation was carried out to evaluate F2 population derived from WH711 and WH542 varieties of wheat for reaction to yellow rust, traits contributing to yield and SSR analysis. WH711 (susceptible to yellow rust) × WH542 (resistant to yellow rust) F2 population when screened against yellow rust reaction, fitted in asegregating ratio of 9:3:3:1 for highly resistant, moderately resistant, moderately susceptible and highly susceptible plants, suggesting the digenic effect (two gene segregation) controlling the respective trait. Correlation analysis revealed that biological yield per plant (0.891) had the highest direct contribution towards grain yield per plant followed by number of tillers per plant (0.816). Out of 100 SSR markers used, 81 SSRs showed amplification, out of which 17 SSRs (including four Yr specific SSRs specific for Yr genes viz. Yr1, Yr16, Yr36, YrH52) produced polymorphic bands while 64 SSRs produced monomorphic bands. Size of amplified products in two different genotypes ranged from 86 bp to 288 bp. WH711 × WH542 plants showed heterozygosity of 38% and homozygosity of 62%. Though no marker was found linked to the rust resistance but NTSYS-PC UPGMA cluster tree analysis led to the grouping of 120 F2 plants in two major groups at a similarity coefficient of 0.55, major group I containing 22 F2 plants and WH711while major group II containing 97 F2 plants and WH542, thus indicating F2 population is more inclined towards WH542.
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    ThesisItemOpen Access
    Molecular marker analysis for bacterial blight resistance in cluster bean (Cyamopsis tetragonoloba (L.) Taub.)
    (CCSHAU, 2014) Amandeep Kaur; Yadav, Neelam R.
    Cluster bean (Cyamopsis tetragonoloba) is an important member of Leguminoseae family and is a rich source of galctomannan content making it an industrial crop. In this crop, there are almost no genomic resources or high quality co-dominant markers available for genetic analysis. At present there is no report of SSR markers targeting bacterial leaf blight resistance available for this crop but available EST database was used to develop EST-SSRs. Cluster bean EST sequences (16476) were retrieved and used to identify EST-SSRs using SSRIT tool. A total of 858 SSRs were identified with maximum proportion of trinucleotide repeats and least proportion of pentanucleotide repeats. CT/TC motif was most abundant. Eighty sequences related to pathogen stress response were identified out of which 17 SSRs were used for in silico primer designing. Twenty seven genotypes were selected for molecular marker analysis using these 11 EST-SSRs. Plants were also grown in net house for studying disease incidence. Two wild speices, C. senegelensis (18.55 ± 1.86 %) and C. serrata (16.30 ± 0.93 %) showed lowest disease incidence, while highest disease incidence was observed in case of Pusa Nav Bahar (73.23 ± 0.92 %). All the 11 EST-SSR markers were polymorphic in molecular analysis, showing different allele sizes in wild and cultivated genotypes. Diversity analysis suggested that two wild species formed a different group at a similarity coefficient of 0.75 including some of the cultivated genotypes. Four genotypes, C. senegelensis, HG 563, HG 75 and PNB, were selected for sequencing and multiple sequence alignment against HES 1401. The alignment suggested the presence of SNPs and In/ Dels in the sequences