Plant regeneration in cultivated and wild species of cluster bean (Cyamopsis spp.)
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Date
2016
Authors
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Publisher
CCSHAU
Abstract
Cluster bean (Camopsis tetragonoloba L. Taub) commonly known as guar, is a hardy crop and
considered as green gold due to galactomannan content.The present study was undertaken to observe
the response of various explants viz., immature cotyledon, immature embryo, Cotyledonary node and
hypocotyl of three genotypes HG2-20, HG 563 and C. serrataShinz.on different growth regulators and
concentrations. The Cultured cotyledonary node and hypocotyl explants (15 days old) were taken from
in vitro grown seedlings andimmature cotyledon, immature embryo axes explants taken fron pods. A
total of 13, MS modified media supplemented with different concentrations of growth regulators TDZ,
BAP, Kin and NAA were used for in vitro culture of explants. MS medium supplemented with TDZ
alone and TDZ in combination with NAA was the most responsive medium for shoot regenerationin all
three genotypes.Among the various explants, Cotyledonary node explants werefound to be the most
responsive.Hypocotyl explants did not show shoot formation in all three genotypes. Both immature
cotyledon and immature embryo axes explants showed good response for shoot regeneration on MS
medium supplemented with TDZ (5 μM) with NAA (2 mg/l).The best shoot regeneration response
(99.66%) from cotyledonary node explants was observed on MS medium containing TDZ (5μM) in C.
tetragonoloba cv. HG 2-20. Maximum shoots per explant (Eight) was observed from embryo axes
explants of C. serrata on MS medium supplemented with TDZ (5 μM). A total of Four MS modified
media were used for rooting. None of media induced roots in all three genotypes.
Description
Keywords
Auxins, Biological phenomena, Regeneration, Planting, Beans, Cytokinins, Biological development, Vegetative propagation, Concentrates, Genotypes