Micronutrient and SSR marker analysis of pearl millet [Pennisetum glaucam (L.) R. Br.] inbred lines
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Date
2015
Authors
Sangwan, Sonali
Journal Title
Journal ISSN
Volume Title
Publisher
CCSHAU
Abstract
A set of thirty-six pearl millet inbred lines was used to study genetic variability and
correlation analysis for seven morphological characters namely days to 50% flowering, plant height
(cm), ear length (cm), ear diameter (cm), 1000 grain weight (g), grain yield per plant (g) and dry fodder
yield per plant (g) along with micronutrient (Fe and Zn) status. The pearl millet inbred lines were
evaluated in randomized block design with two replications in two environments during 2014. Analysis
of variance for all the characters under study showed significant differences indicating the presence of
genetic variability among the inbreds. The estimates of variability parameter for grain yield,
contributing traits and Fe and Zn content revealed the high PCV value as compared to GCV value that
suggests the role of environment in the expression of all these characters. An additive gene action was
indicated for dry fodder yield per plant, grain yield per plant, Fe and Zn content by high to moderate
heritability and genetic advance as per cent of mean values. Correlation analysis showed highly
significant correlation between grain Fe and Zn content which signifies simultaneous improvement in
the two traits. Grain yield per plant showed no significant negative correlation with Fe and Zn that
suggests improvement in nutrient value without sacrificing yield. A set of 30 SSR primers was also
used for molecular diversity assessment in 36 pearl millet inbred lines. Mean allele per locus and PIC
obtained was 10.5 and 0.796, respectively. Cluster analysis distributed these genotypes into 9 clusters
where 4 genotypes failed to fall in any of these clusters.
Description
Keywords
Pearl millet. Genetic diversity, Micronutirent, SSR marker