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Govind Ballabh Pant University of Agriculture and Technology, Pantnagar

After independence, development of the rural sector was considered the primary concern of the Government of India. In 1949, with the appointment of the Radhakrishnan University Education Commission, imparting of agricultural education through the setting up of rural universities became the focal point. Later, in 1954 an Indo-American team led by Dr. K.R. Damle, the Vice-President of ICAR, was constituted that arrived at the idea of establishing a Rural University on the land-grant pattern of USA. As a consequence a contract between the Government of India, the Technical Cooperation Mission and some land-grant universities of USA, was signed to promote agricultural education in the country. The US universities included the universities of Tennessee, the Ohio State University, the Kansas State University, The University of Illinois, the Pennsylvania State University and the University of Missouri. The task of assisting Uttar Pradesh in establishing an agricultural university was assigned to the University of Illinois which signed a contract in 1959 to establish an agricultural University in the State. Dean, H.W. Hannah, of the University of Illinois prepared a blueprint for a Rural University to be set up at the Tarai State Farm in the district Nainital, UP. In the initial stage the University of Illinois also offered the services of its scientists and teachers. Thus, in 1960, the first agricultural university of India, UP Agricultural University, came into being by an Act of legislation, UP Act XI-V of 1958. The Act was later amended under UP Universities Re-enactment and Amendment Act 1972 and the University was rechristened as Govind Ballabh Pant University of Agriculture and Technology keeping in view the contributions of Pt. Govind Ballabh Pant, the then Chief Minister of UP. The University was dedicated to the Nation by the first Prime Minister of India Pt Jawaharlal Nehru on 17 November 1960. The G.B. Pant University is a symbol of successful partnership between India and the United States. The establishment of this university brought about a revolution in agricultural education, research and extension. It paved the way for setting up of 31 other agricultural universities in the country.

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  • ThesisItemOpen Access
    X - sperm enrichment in buffalo bull semen by percoll density gradient
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2018-08) Rawat, Meenakshi; Sharma, Mridula
    The present research work was conducted to investigate the effect of percoll density gradient method on quality of enriched semen and quantify the X-sperm enrichment by Raman spectroscopy in buffalo bull semen. Semen samples collected by artificial vagina method from murrah buffalo bull with mass motility ˃+3 and progressive motility ˃70% were considered for the study. Fresh semen was divided into5 groups. The groups were control (fresh semen), centrifuged semen (supernatant), centrifuged semen (pellet), 7 layer (70-10%) percoll gradient centrifuged semen (supernatant) and 7 layer (70-10%) percoll gradient centrifuged semen (pellet). The 7 layered percoll density gradient medium was composed of 70 -10% percoll gradients with dense layer at bottom and lighter layer at top. Both the centrifugations were done at 750RCF for 20 minutes at 24ºC. Thereafter, the groups were diluted in either PBS or EYC dilutor after centrifugation and were sent to UGC-DAE Consortium for Scientific Research, Indore for Raman spectroscopy to check the separation efficiency in buffalo bull semen.The samples which were sent had equal volume (400 μl) and equal no. of sperms (2 x 106) and Raman spectroscopy was done at 100X magnification using 25w power for 30sec at 473nm wavelength with wave number ranging from 500 to 1800 cm-1 to detect the best method for X sperm enrichment and also to estimate the percentage increment of X sperms by percoll density gradient method in buffalo bull semen. Results concluded that the 7 layered 70% percoll density gradient method is good for X sperm enrichment and showed enhanced results compared to control i.e. the X sperm in 7 layer 70% percoll pellet was having more than 50% sperm count in EYC diluted semen. So quality assessment for various seminal parameters like volume, pH, motility, live percentage, sperm abnormality, concentration, metabolic activity (MBRT), membrane integrity (HOST) and acrosomal integrity was done for the 7 layered 70% percoll density gradient to evaluate the effect of percoll density gradient on quality of semen. The concentration, progressive motility, pH and MBRT decreased significantly in all the groups with highest value (P<0.05) for percoll pellet after fresh semen and lowest for centrifuged supernatant. The time taken to reduce methylene blue in all the groups was significantly (P<0.05) higher compared to group І. The percent live sperms and HOST showed similar responses. The sperm abnormality was significantly higher for percoll supernatant followed by centrifuged supernatant, centrifuged pellet, percoll pellet and was lowest for fresh semen. There was no significant effect on percent of spermatozoa with partially damaged acrosomal membrane and fully damaged acrosomal membrane in all five groups however, partially damaged acrosomal membrane sperms were significantly (P<0.05) more than the fully damaged acrosomal membrane sperms. In conclusion 7 layered 70% percoll density gradient method is good in both X sperm enrichment and sperm quality and can be used to enhance female progeny production.
  • ThesisItemOpen Access
    Effect of different surgical approaches on various physiological and hemato-biochemical parameters before and after caesarean section in cows suffering from dystocia
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2018-08) Tiwari, Prashant; Gupta, H.P.
    Caesarean section is a surgical procedure in which abdominal wall and uterus is incised to deliver the foetus when other methods fail. The present work was designed to study the effect of recumbent left oblique ventro lateral (group A) and caudal para median (group B) incision sites on cases of dystocia undergoing caesarean section in cows (n=6 each) at T.V.C.C of G.B.P.U.A.T, Pantnagar-263145, District Udham Singh Nagar (Uttarakhand) and T.V.C.C of L.U.V.A.S Hisar, Haryana-125001, which were compared with normal calving animals (n=6) as control in which no surgical intervention was done. At first the physiological parameters were taken followed by blood sample collection to know different parameters of blood and serum, before caesarean, 0, 24, 48 and 72 hours after caesarean section and normal calving in control group. Among physiological parameters heart rate and pulse rate restored early in group A, rectal temperature and respiration rate restored in both the groups simultaneously when compared to control. Among hematological parameters, haemoglobin and PCV values were within normal physiological range in both the caesarean groups till period of study. Near normal vales of ESR and TEC restored early in group A, while TLC restored just after caesarean operation in all the groups. Neutrophilia and eosinopenia was found evident in both the caesarean groups till the duration of study. Basophils and monocytes were found in normal physiological range at all time intervals in all the groups. Lymphocytes restored to normal values in both the caesarean groups simultaneously when compared to control. Among biochemical parameters studied, total protein, phosphorus, sodium, potassium, and albumin re-established to near normal values early in group A, whereas glucose, ALT and creatinine restored in both the caesarean groups at same time. AST, BUN and globulin values did not came to normal till period of study in both the caesarean groups. Not much difference was found in the time of onset of estrum after the caesarean operation in cows suffering from dystocia when compared with cows undergoing normal parturition. In our findings recumbent left oblique ventro lateral is a better site to perform the operation.
  • ThesisItemOpen Access
    Separation of X and Y sperms in bovine semen and their detection following enrichment
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2018-08) Chaudhary, Deeksha; Sharma, Mridula
    The present study was conducted to observe the effect of percoll density gradient method on X-sperm enrichment and quality of semen. It was performed at Semen Production Centre, CVASc, GBPUAT, Pantnagar, Uttarakhand. Ejaculates were collected by AV method from Crossbred and Sahiwal bulls. Xsperm enrichment was done by percoll density gradient method. Three types of gradients were prepared i.e. 7 layers (70-10%), 7 layers (80-20%) and 3 layers (30%, 505 and 70%). Semen (1ml or 3ml) was placed over the gradient in conical centrifuge tube. Centrifugation was done at 750 xg (22-24°C) for 15 min. Thus, Raman spectroscopy was performed in different groups. Those were fresh semen (Control), 7 layers (70%) (G III), 7 layer (80%) (G IV) and 3 layer (70%) (G V). The pellets obtained were diluted in PBS or EYC medium. Raman peaks (DNA specific) were not much clear for PBS diluted though intensities were highest for 7 layer (70%). Thus, quantification of X-sperms in enriched EYC diluted semen was done by calculating and comparing area at 785, 1095 and 1581 cm-1. There was 26-30% and nearly 17-20% X-sperm enrichment in 7 layer (70%) percoll centrifugation compared to control in Crossbred and Sahiwal, respectively. pH of percoll density gradient centrifuged (supernatant) decreased significantly (P<0.05) in Crossbred semen. Progressive motility of percoll density gradient centrifuged (supernatant) decreased significantly (P<0.05) in Crossbred semen. Live spermatozoa (%) of enriched semen (pellet) were increased in Crossbred and Sahiwal semen. Acrosome integrity was not affected in all the groups in Crossbred bull whereas intact sperm decreased significantly (P<0.05) in percoll density gradient centrifuged supernatant of Sahiwal semen. HOST responsive sperms were not affected in all the groups of Crossbrd and Sahiwal semen. X-sperms enrichment of 29.9% and 20.48% in Crossbred and Sahiwal semen was achieved by percoll density gradient method (7layer 70%). Semen quality of enriched semen was also not affected. Thus semen enriched by percoll density gradient method (7 layer 70%) can be used to increase female calves birth after A.I.
  • ThesisItemOpen Access
    Studies on dietary manipulation with PUFA on embryo production and conception in cattle
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2018-08) Sahu, Mukesh; Shiva Prasad
    The present study was designed to study the effect of supplementation of PUFA enriched diet (Flax seed @ 300 g/100 kg body weight/animal/day for 21 days during estrous cycle) on embryo production in donor, hormonal (Progesterone), biochemical profile (glucose and cholesterol) in donor, recipient and conception rate in crossbred embryo recipient following embryo transfer from Sahiwal donor. Donor (n=6) and Crossbred cows (n= 24) were selected and divided into four groups, control (no treatment), T1 (treatment given to recipient only), T2 (treatment given to donor only) and T-3 (both were treated). Blood samples were collected on day 0, 5, 10, 15 and 20 of estrous cycle in donors and on day 0, 7, 14 and 21 of estrous cycle in recipients. The serum was separated and stored at -20 degree celcius in refrigerator till analysis of hormone and biochemical parameters. The superovulatory response, mean ovulation rate (number of C.L.), mean total of embryo/ova recovered, recovery rate and average transferable embryos was 75% and 100%, 12.25 ± 2.70 and 3.5 ± 1.54, 12 ± 0.71 and 7.5 ± 1.60, 28.57% and 62.5 % and 49.99 % and 80 % in non treated (control + T-1) and treated (T-2 + T-3) groups respectively. The mean ovulation rate (number of C.L.) and the mean total of embryo/ova recovered differed non significantly (p>0.05). The mean concentration of serum glucose and serum cholesterol differ significantly (p<0.05) but mean concentration of serum progesterone differs non significantly (p>0.05) in respective groups. In recipients, the mean serum glucose concentration differ significantly (p<0.05) and in mean serum cholesterol concentration no significant (p>0.05) difference was found in between non treated (control + T-2) and treated (T-1 + T-3) groups. There was increase in value of cholesterol in treated group than non treated group reported. The mean serum progesterone concentration on day 0 and 7 of estrous cycle differ non significantly (p>0.05) between all groups. On day 14 significant (p>0.05) difference was found in between T-3 and other groups and on day 21 significant (p>0.05) difference was found between non treated (control + T-2) and treated (T-1 +T-3) groups. On the basis of our results, it can be concluded that flax seeds supplementation is effective in increasing number of embryo production, glucose and cholesterol concentration in donor and recipient and progesterone concentration in recipients animals during luteal phase of estrous cycle, thus it may be beneficial in improving conception rate following embryo transfer.
  • ThesisItemOpen Access
    Studies on therapeutic efficacy of various immunomodulators on endometritis in repeat breeding crossbred cows
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2017-12) Singh, Bhoopendra; Gupta, H.P.
  • ThesisItemOpen Access
    Incidence, etiology, treatment and recovery of dystocia in bovines
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2017-08) Rana, Devendra Singh; Gupta, H.P.
    The present study was designed to study the incidence of dystocia in various reproductive disorders present in bovines in Tarai region of Udham Singh Nagar and Nainital districts of Uttarakhand. , which indicated 14.62 % incidence of the dystocia in the study area. Also to study the physiological, hematological and biochemical changes occurred in dystocia cases of cows and buffaloes immediately before and after the correction through various methods like caesarean operation, traction, fetotomy, rolling plus traction, rolling plus hormonal and hormonal. In cattle, the maximum of incidence of dystocia was recorded in winter season followed by summer and rainy season. However, in buffaloes, the maximum incidence of dystocia was recorded during rainy season followed by summer and winter season. In present study, total 37 cases of dystocia were recorded. Out of these, 7 cases were corrected through caesarean section, 5 cases corrected through fetotomy, 5 cases corrected through traction, 10 cases corrected through rolling plus traction, 5 cases corrected through rolling plus hormonal and 5 cases corrected through hormonal method. By applying these techniques in dystocia affected animals, 25(67.57%) males and 12 females (32.43%) calves were delivered. Out of which, 13(35.13%) live and 24 (64.86%) dead calves were found. In dystocia, physiological, hematological and biochemical parameters variations were recorded before and after the correction of dystocia in animals. The physiological status of the animal assessed through rectal temperature, heart rate, pulse rate and respiration rate. The hematological and biochemical parameters analyzed in our study were TEC, TLC, Hemoglobin, PCV, lymphocyte, neutrophil, monocyte, eosinophil, basophil total protein, calcium, phosphorus, glucose, cholesterol, cortisol, BUN, ALT, AST, and creatinine. The cortisol was significantly higher during before and after the correction in all the groups. Glucose was significantly higher during before and after the correction of dystocia with rolling plus traction, rolling plus hormonal and hormonal methods. ALT and AST was significantly higher in traction and rolling plus hormonal methods of correction. The value of creatinine significantly lower before the correction of dystocia.
  • ThesisItemOpen Access
    Studies on improving conception following embryo transfer in cattle
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2017-08) Maithani, Mayank; Shiv Prasad
    The present study was designed to study the effect of tolfenamic acid on hormonal (Progesterone, PGFM), biochemical profile and conception rate in crossbred embryo recipient following embryo transfer from Sahiwal cows. Crossbred cows were selected and divided into three groups, control, T1 (tolfenamic acid @4mg/kg b.wt, I.M 1 hr before transfer) and T2 (tolfenamic acid @4mg/kg b.wt, I.M 1 hr before transfer and during 15, 16 days of estrous cycle). Blood samples were collected on 7,15,16,17 and 32nd days of estrous cycle. The serum was separated and stored at -20 degree celcius in refrigerator till analysis of hormones and biochemical parameters. The superovulatory response was better as number of C.L, Total embryo (TE), Blastocyst (Bl), Morula (M) and 8-16 cell and degenerated embryo per superovulatory treatment (ST) were 15.11±0.96, 4.25±0.98, 4±0.94, 1, 1 and 1 respectively. However the embryo recovery rate was 25%. The mean values of serum protein, glucose and cholesterol did not differ significantly between different groups .However, the mean value of serum urea and creatinine increases significantly in T2 compared with T1 and control. The mean value of serum PGFM decreased significantly in T2 group during all days of sample collection and progesterone concentration increased in T2 group.In present study total 3 pregnancy were achieved ,one in each group with overall conception rate of 16.6%. On the basis of our results, it can be concluded that tolfenamic acid is effective in reducing PGFM and increasing progesterone concentration during luteal phase of estrous cycle, thus it may be beneficial in improving conception rate following embryo transfer as evident by PGFM and progesterone. However, no significant alterations could be recorded in biochemical parameters following tolfenamic acid administration. More numbers of conceptions are required to finally validate the findings and to recommend for future application.
  • ThesisItemOpen Access
    Characterization of bubaline epididymal spermatozoa stored at 5°c in-situ and their storage resistance in modified citrate dilutors
    (2017-08) Rana, Ramkrishan; Gupta, H.P.
    The present study was undertaken to determine the testicular biometry of slaughtered buffalo bull testis and its correlation with sperm production, to find out the improvement in keeping quality of epididymal spermatozoa in egg yolk citrate diluter modified with alanine, cysteine and glutamine and to evaluate the effect of amino acid on structural aspect and storage resistance of spermatozoa through initial motility, live count, percent abnormality and HOS reacted spermatozoa. The 48 testicles from slaughtered buffalo bulls were collected from slaughter house, and divided into 4 groups each containing 12 testicles; G1 (semen collection at 0 hours of testicle storage), G2 (semen collection at 24 hours of testicle storage), G3 (semen collection at 48 hours of testicle storage) and G4 (semen collection at 72 hours of testicle storage). The testicles were processed on the same day and evaluated for morphological traits and seminal characteristics. The epididymal semen was collected from refrigeration storage (5ºC) of testicles at 0, 24, 48 and 72 hours and further percent initial motility, livability, abnormality and HOS positive spermatozoa was evaluated . The biometric testicular parameters (testicular length with epididymis and without epididymis, circumference, diameter, weight, volume, caput length, width and weight, corpus length, width and weight, cauda length, width and weight) were measured and significant (P<0.05) difference between left and right testis was observed The significant (P<0.05) variation in seminal attribute was observed among testicles as well as among the groups. The post thaw semen quality was assessed 24, 48 and 72 hour after refrigeration of epididymal semen. A significant improvement in post thaw seminal characteristics and HOS reactive sperm was observed among the treatments. Incorporation of glutamine @ dose of 25 mM and cysteine @ 5 mM in diluter resulted in maximum increase in post thaw % motility, live sperm, post thaw HOS positive sperm as compared to alanine and control group. Glutamine and cysteine, promising semen additive which can be used further to improve the quality of refrigerated thawed semen. The data was processed using statistical analysis programme.
  • ThesisItemOpen Access
    Study on flunixin meglumine to improve conception following embryo transfer in cattle
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2017-08) Malik, Ankit; Shiv Prasad
    The present study was designed to study the effect of flunixin meglumine during embryo transfer and at the time of MRP on PGFM, progestrone and biochemical profile in embryo recipient cows. Crossbred donor cow (n=5) treated with 240 mg of FolltropinV/animal, as a total dose and given at 12 hours interval in 8 divided doses in decreasing order i.e. 45:45, 35:35, 25:25, 15:15 mg, morning and evening, by IM route , each cow was superovulated twice. Further, crossbred cows (n=18) were selected and divided into three groups; control, Control (n=6) : NSS was given, T-I (n=6) : flunixin meglumine @ dose rate of 1.1 mg/kg was given half hour before transfer, T-II (n=6) : flunixin meglumine @ dose rate of 1.1 mg/kg was given half hour before transfer and on 16th and 17th day of standing estrus. Blood sample collection was done; Before transfer i.e. on 7½ day of estrus without any treatment then half hour after treatment. After transfer, at 15 minute, 50 minute and 150 minute blood collection was done. On 16th, 17th and 18th day of estrus blood was collected after every 6 hour interval and then on 32th day of standing estrus for protein, glucose, cholesterol, creatinine and urea. For PGFM on day of transfer blood sample collection was same as above but on day 16,17and 18 of estrus cycle blood sampling was done after every 12 hour and for progestrone estimation blood samples were taken before treatment/transfer, 150 min after transfer/treatment then on 16th , 17th , 18th , and 32nd day of estrus. The overall superovulatory response, mean ovulation, total embryo/ova recovered and average transferable embryos recovered were 90%, 9.6±1.84, 6.3±1.84 and 60.41% respectively. There was no significance difference (p>0.05) in protein, glucose, cholesterol and creatinine serum level but there was slight decrease in protein level while creatinine level increased in treated group. Serum urea concentration differ significantly (p<0.05) between the groups after treatment. The PGFM level and progesterone level also differ non significantly (p>0.05) between the groups but after treatment there was marked decrease in PGFM level and simultaneously progestrone concentration increased. One animal aborted at 76 days and two became pregnant. On the basis of above findings, it may be concluded that administration of flunixin meglumine during luteal of estrous cycle may be beneficial in improving conception following embryo transfer in crossbred cattle as evident by decreased level of PGFM and increased level of progestrone during critical days of maternal recognition of pregnancy.