Studies on dietary manipulation with PUFA on embryo production and conception in cattle
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Date
2018-08
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G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
The present study was designed to study the effect of supplementation of PUFA enriched diet (Flax seed @ 300 g/100 kg body weight/animal/day for 21 days during estrous cycle) on embryo production in donor, hormonal (Progesterone), biochemical profile (glucose and cholesterol) in donor, recipient and conception rate in crossbred embryo recipient following embryo transfer from Sahiwal donor. Donor (n=6) and Crossbred cows (n= 24) were selected and divided into four groups, control (no treatment), T1 (treatment given to recipient only), T2 (treatment given to donor only) and T-3 (both were treated). Blood samples were collected on day 0, 5, 10, 15 and 20 of estrous cycle in donors and on day 0, 7, 14 and 21 of estrous cycle in recipients. The serum was separated and stored at -20 degree celcius in refrigerator till analysis of hormone and biochemical parameters. The superovulatory response, mean ovulation rate (number of C.L.), mean total of embryo/ova recovered, recovery rate and average transferable embryos was 75% and 100%, 12.25 ± 2.70 and 3.5 ± 1.54, 12 ± 0.71 and 7.5 ± 1.60, 28.57% and 62.5 % and 49.99 % and 80 % in non treated (control + T-1) and treated (T-2 + T-3) groups respectively. The mean ovulation rate (number of C.L.) and the mean total of embryo/ova recovered differed non significantly (p>0.05). The mean concentration of serum glucose and serum cholesterol differ significantly (p<0.05) but mean concentration of serum progesterone differs non significantly (p>0.05) in respective groups. In recipients, the mean serum glucose concentration differ significantly (p<0.05) and in mean serum cholesterol concentration no significant (p>0.05) difference was found in between non treated (control + T-2) and treated (T-1 + T-3) groups. There was increase in value of cholesterol in treated group than non treated group reported. The mean serum progesterone concentration on day 0 and 7 of estrous cycle differ non significantly (p>0.05) between all groups. On day 14 significant (p>0.05) difference was found in between T-3 and other groups and on day 21 significant (p>0.05) difference was found between non treated (control + T-2) and treated (T-1 +T-3) groups. On the basis of our results, it can be concluded that flax seeds supplementation is effective in increasing number of embryo production, glucose and cholesterol concentration in donor and recipient and progesterone concentration in recipients animals during luteal phase of estrous cycle, thus it may be beneficial in improving conception rate following embryo transfer.
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