Study on flunixin meglumine to improve conception following embryo transfer in cattle
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Date
2017-08
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G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
The present study was designed to study the effect of flunixin meglumine during embryo transfer and at the time of MRP on PGFM, progestrone and biochemical profile in embryo recipient cows. Crossbred donor cow (n=5) treated with 240 mg of FolltropinV/animal, as a total dose and given at 12 hours interval in 8 divided doses in decreasing order i.e. 45:45, 35:35, 25:25, 15:15 mg, morning and evening, by IM route , each cow was superovulated twice. Further, crossbred cows (n=18) were selected and divided into three groups; control, Control (n=6) : NSS was given, T-I (n=6) : flunixin meglumine @ dose rate of 1.1 mg/kg was given half hour before transfer, T-II (n=6) : flunixin meglumine @ dose rate of 1.1 mg/kg was given half hour before transfer and on 16th and 17th day of standing estrus. Blood sample collection was done; Before transfer i.e. on 7½ day of estrus without any treatment then half hour after treatment. After transfer, at 15 minute, 50 minute and 150 minute blood collection was done. On 16th, 17th and 18th day of estrus blood was collected after every 6 hour interval and then on 32th day of standing estrus for protein, glucose, cholesterol, creatinine and urea. For PGFM on day of transfer blood sample collection was same as above but on day 16,17and 18 of estrus cycle blood sampling was done after every 12 hour and for progestrone estimation blood samples were taken before treatment/transfer, 150 min after transfer/treatment then on 16th , 17th , 18th , and 32nd day of estrus.
The overall superovulatory response, mean ovulation, total embryo/ova recovered and average transferable embryos recovered were 90%, 9.6±1.84, 6.3±1.84 and 60.41% respectively. There was no significance difference (p>0.05) in protein, glucose, cholesterol and creatinine serum level but there was slight decrease in protein level while creatinine level increased in treated group. Serum urea concentration differ significantly (p<0.05) between the groups after treatment.
The PGFM level and progesterone level also differ non significantly (p>0.05) between the groups but after treatment there was marked decrease in PGFM level and simultaneously progestrone concentration increased. One animal aborted at 76 days and two became pregnant. On the basis of above findings, it may be concluded that administration of flunixin meglumine during luteal of estrous cycle may be beneficial in improving conception following embryo transfer in crossbred cattle as evident by decreased level of PGFM and increased level of progestrone during critical days of maternal recognition of pregnancy.
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