Studies on improving conception following embryo transfer in cattle
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Date
2017-08
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G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
The present study was designed to study the effect of tolfenamic acid on hormonal (Progesterone, PGFM), biochemical profile and conception rate in crossbred embryo recipient following embryo transfer from Sahiwal cows. Crossbred cows were selected and divided into three groups, control, T1 (tolfenamic acid @4mg/kg b.wt, I.M 1 hr before transfer) and T2 (tolfenamic acid @4mg/kg b.wt, I.M 1 hr before transfer and during 15, 16 days of estrous cycle). Blood samples were collected on 7,15,16,17 and 32nd days of estrous cycle. The serum was separated and stored at -20 degree celcius in refrigerator till analysis of hormones and biochemical parameters. The superovulatory response was better as number of C.L, Total embryo (TE), Blastocyst (Bl), Morula (M) and 8-16 cell and degenerated embryo per superovulatory treatment (ST) were 15.11±0.96, 4.25±0.98, 4±0.94, 1, 1 and 1 respectively. However the embryo recovery rate was 25%. The mean values of serum protein, glucose and cholesterol did not differ significantly between different groups .However, the mean value of serum urea and creatinine increases
significantly in T2 compared with T1 and control. The mean value of serum PGFM decreased significantly in T2 group during all days of sample collection and progesterone concentration increased in T2 group.In present study total 3 pregnancy were achieved ,one in each group with
overall conception rate of 16.6%. On the basis of our results, it can be concluded that tolfenamic acid is effective in reducing PGFM and increasing progesterone concentration during luteal phase of estrous cycle, thus it may be beneficial in improving conception rate following embryo transfer as evident by PGFM and progesterone. However, no significant alterations could be recorded in biochemical parameters following tolfenamic acid administration. More numbers of conceptions are required to finally validate the findings and to recommend for future application.
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