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Anand Agricultural University, Anand
Anand Agricultural University (AAU) was established in 2004 at Anand with the support of the Government of Gujarat, Act No.(Guj 5 of 2004) dated April 29, 2004. Caved out of the erstwhile Gujarat Agricultural University (GAU), the dream institution of Sardar Vallabhbhai Patel and Dr. K. M. Munshi, the AAU was set up to provide support to the farming community in three facets namely education, research and extension activities in Agriculture, Horticulture Engineering, product Processing and Home Science. At present there seven Colleges, seventeen Research Centers and six Extension Education Institute working in nine districts of Gujarat namely Ahmedabad, Anand, Dahod, Kheda, Panchmahal, Vadodara, Mahisagar, Botad and Chhotaudepur
AAU's activities have expanded to span newer commodity sectors such as soil health card, bio-diesel, medicinal plants apart from the mandatory ones like rice, maize, tobacco, vegetable crops, fruit crops, forage crops, animal breeding, nutrition and dairy products etc. the core of AAU's operating philosophy however, continues to create the partnership between the rural people and committed academic as the basic for sustainable rural development. In pursuing its various programmes AAU's overall mission is to promote sustainable growth and economic independence in rural society. AAU aims to do this through education, research and extension education. Thus, AAU works towards the empowerment of the farmers.
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ThesisItem Open Access “Identification and validation of internal control genes for quantitative gene expression studies in pearl millet under biotic stress (downy mildew infection) and abiotic stress (salt stress(Anand Agricultural University, Anand, 2012) POONAM D. KANANI; Dr. Y. M. ShuklaPearl millet (Pennisetum glauccum (L.) R. Br.) is an annual, cross pollinating, monocot crop belonging to the family Poaceae and sub family Penicedae. It is the sixth most important cereal of the world and has critical value as food security in some of the world’s hottest and driest cultivated areas of Africa and Asia. However, the crop faces constraints to the productivity which includes the prevalence of different biotic and abiotic stresses. The present work was carried out with an aim to identify and select endogenous genes for pearlmillet under biotic stress (downy mildew) and abiotic stress (salinity) and validating constitutively expressed genes in pearlmillet through RT-PCR and analyzed it through geNorm for best stable reference geneThesisItem Open Access VALIDATION OF ENDOGENOUS GENES IN RICE (Oryza sativa L.) UNDER DROUGHT AND SALT STRESS”(Anand Agricultural University, Anand, 2012) ANUCHITHRA V.L.; Dr. Subhash N.The use of suitable and stable reference genes for normalization is an important and unavoidable step during gene expression studies in relative quantification using Real Time PCR. Validation of endogenous genes in rice (Oryza sativa L.) under drought and salt stress was carried out with an aim to identify most suitable reference genes for the normalization of gene expression data in rice. After four days of drought and salinity stress the resistant cultivars showed higher RWC as compared to the susceptible cultivars. The drought susceptible cultivar, GR 3 showed higher leaf rolling index (LRI) as compared to the resistant cultivar AAUDR 1. Based on the visual symptoms of salt damage the tolerant cultivar Dandi scored 3 in the Standard Evaluation System (SES) and susceptible cultivar GR 11 scored 7, indicating tolerance and susceptible reactions, respectively. cDNA preparation was carried out from total RNA of rice seedlingsThesisItem Open Access “Molecular studies and genetic diversity analysis in Brassica species using microsatellite and RAPD markers”(Anand Agricultural University, Anand, 2013) PRAVIN PRAJAPAT O; Dr. N. SasidharanThe Brassicaceae (Cruciferae) or mustard family includes many economically important edible and industrial oilseed, vegetable, condiment, and fodder crops. Brassicas are the third most important oilseed crop in the world after soybean and palm. China, India, Canada, Japan and Germany are the major rapeseed-mustard growing countries. In India, total area under mustard cultivation is 6.39 million ha (2012-13) and production 7.41 million tonnes, whereas in Gujarat, it is 2.04 lakh ha (2012-13) and production of 71.12 lakh tonnes. The present study was conducted to characterize as well as assess the diversity existing in 30 mustard genotypes belonging to different species such as B. juncea, B. rapa, B. napus and B. carinata using Random Amplified Polymorphic DNA (RAPD) and Simple Sequence Repeats (SSR) markers. The genomic DNA was isolated from mustard leaves at seedling stage using Cetyl Trimethyl Ammonium Bromide (CTAB) method (Murray and Thompson, 1980). AThesisItem Open Access “MOLECULAR CHARACTERIZATION OF PIGEONPEA [ Cajanus cajan (L.) Millsp.] GENOTYPES FOR Fusarium WILT RESISTANCE(Anand Agricultural University, Anand, 2013) PRAJAPATI VIJAYKUMAR I; Dr. N. SasidharanPigeonpea or Red Gram [Cajanus cajan (L.) Millsp.] (2n = 22) is the second most important pulse crop and plays an important role in subsistence agriculture. In Gujarat, total area under pigeonpea cultivation was 2.65 lakh hectares with an annual production of 2.94 lakh tonnes and productivity of 1109.43 kg/ha (Anon, 2011). In Gujarat, the fusarium wilt is one of the major diseases of concern followed by Phytophthora blight and sterility mosaic, affecting the productivity of this crop per unit area. Therefore, to minimize yield losses due to fusarium wilt, it is necessary to tackle these problems at molecular level by developing cultivars which resist/tolerate these biotic stresses and have greater recovery from damage. Therefore efforts have been made in the present study to characterize as wellThesisItem Open Access ‘‘DIFFERENTIAL GENE EXPRESSION STUDY ON DROUGHT STRESS RESPONSIVE GENES AND VALIDATION IN PEARLMILLET (Pennisetum glaucum L.)’’(Anand Agricultural University, Anand, 2013) PATEL HELI J.; Dr. Y. M. ShuklaPearlmillet (Pennisetum glaucum L.) is an annual, cross pollinating, monocot crop belonging to the family Poaceae and sub family Penicedae. It is widely grown as a multi-purpose cereal grain crop for feed, fodder, fuel and mulch over more than 26 million hectares predominantly in the semi-arid tropics of sub-Saharan Africa and the Indian subcontinent. Water deficit is one of the major factors limiting crops production in the world. Despite the fact that pearl millet is considered as a drought tolerant crop it suffers substantial yield losses due to stress occurring at the end of growing season. Thus, the yield enhancement under stress condition requires a better understanding of the mechanisms involved in drought tolerant genotypes. The present work was carried out with aim to validate theThesisItem Open Access “GENETIC DIVERSITY ANALYSIS AMONG GENOTYPES OF DIFFERENT OCIMUM SPECIES USING RAPD AND ISSR MOLECULAR MARKERS”(Anand Agricultural University, Anand, 2013) PATEL HARDIKKUMAR KAUSHIKBHAI; Dr. R. S. FougatOcimum belongs to the Lamiaceae family, which has close to 252 genera and 6700 species, most of which are used for medicinal purpose owing to their chemical properties. Ocimum species has a wide distribution, covering the entire Indian sub continent, ascending up to 1800 meter in the Himalayas and as far as the Andaman and Nicobar islands in the Indian Ocean. It is characterized by more than two basic chromosome numbers and a variety of haploid chromosome numbers (12, 13, 16, 20, 24, 32, 36 and 38). It has numerous medicinal properties like antimicrobial, antifertility, anticancer, antidiabetic, antifungal, antitussive, antiemetic, antispasmodic and antitussiveThesisItem Open Access DIFFERENTIAL GENE EXPRESSION STUDY FOR HEAT, DROUGHT AND COMBINE HEAT AND DROUGHT IN WHEAT (Triticum aestivum L.) GENOTYPE”(Anand Agricultural University, Anand, 2012) RAFALIYA RUTULKUMAR VALLABHBHAI; Dr. Y. M. ShuklaaWheat (Triticum spp.) is self pollinated cereal crop, belonging to the family Poaceae (grasses) and is the most important and universally grown of all food grains having its origin in south western Asia. It is estimated that one third of the world’s population depend on wheat for their nutritional requirements. Abiotic stress is the primary reason for farm production loss worldwide, causing average yield reductions of more than 50%, in wheat (Triticum aestivum). The present investigation was carried out with an aim to study the candidate genes responsible for resistance against heat and drought in wheat and comparison of gene expression between control and stress tissues with in susceptible and resistant genotypes through RT-PCR. Genotypes differing in degree of sensitivity to drought, heat, combine heat and drought stress selected were GW11 and GW273. The study was conducted with objectives for screening of Hsps genes under heat, drought and combine heat & drought stress and also to study the differentialThesisItem Open Access “DIFFERENTIAL GENE EXPRESSION STUDY FOR HEAT, DROUGHT AND COMBINE HEAT AND DROUGHT IN WHEAT (Triticum aestivum L.) GENOTYPE”(Anand Agricultural University, Anand, 2012) RAFALIYA, RUTULKUMAR VALLABHBHAI; Dr. Y. M. ShuklaWheat (Triticum spp.) is self pollinated cereal crop, belonging to the family Poaceae (grasses) and is the most important and universally grown of all food grains having its origin in south western Asia. It is estimated that one third of the world’s population depend on wheat for their nutritional requirements. Abiotic stress is the primary reason for farm production loss worldwide, causing average yield reductions of more than 50%, in wheat (Triticum aestivum). The present investigation was carried out with an aim to study the candidate genes responsible for resistance against heat and drought in wheat and comparison of gene expression between control and stress tissues with in susceptible and resistant genotypes through RT-PCR. Genotypes differing in degree of sensitivity to drought, heat, combine heat and drought stress selected were GW11 and GW273. The study was conducted with objectives for screening of Hsps genes under heat, drought and combine heat & drought stress and also to study the differential ii expression of Hsp genes in wheat through real time PCR and bioinformatics tools. Physiological parameters suggested that root length, shoot length and relative water content significantly decreased in stress conditions as compared to control condition in both the genotypes. However, between the genotypes GW273 had high effect various stresses as compared to GW11 for all the physiological parameters. Total RNA concentration ranged from 332.56-630.00 ng/μl. From total RNA, cDNA synthesis was carried out to study differential gene expression. The genes selected were Hsp70, Hsp26.5, Hsp17.3, Hsp16.9, Hsp23.6 and Hsp 101b. Gene expression study revealed that in drought stress Hsp70, Hsp26.5, Hsp16.9 and Hsp17.3 had higher regulation in genotype GW11. Hsp17.3 and Hsp26.5 showed the maximum expression as compared to Hsp70 and Hsp 16.9 in GW11. In response to heat stress Hsp26.5, Hsp16.9 and Hsp17.3 showed upregulation in genotype GW11 where Hsp17.3 reported the maximum gene expression. The study further revealed all six Hsp genes except Hsp101b were influenced by drought and heat stress. Hsp26.5 and Hsp17.3 were highly upregulated by 6.4 and 5 fold as compare to GW273. Overall gene expression study suggested that Hsp 17.3, Hsp26.5 were upregulated in drought, heat and combine drought-heat in wheat genotypes differing in degree of susceptibility to drought, heat, and combine heat and drought.ThesisItem Open Access IDENTIFICATION AND MAPPING OF MOLECULAR MARKERS LINKED TO FRAGRANCE (fgr) GENE IN RICE (Oryza sativa L.)(AAU, 2013) Ravikiran, Rallapalli L.; Dr. Subhash N.