VALIDATION OF ENDOGENOUS GENES IN RICE (Oryza sativa L.) UNDER DROUGHT AND SALT STRESS”
Loading...
Date
2012
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Anand Agricultural University, Anand
Abstract
The use of suitable and stable reference genes for normalization is
an important and unavoidable step during gene expression studies in
relative quantification using Real Time PCR. Validation of endogenous
genes in rice (Oryza sativa L.) under drought and salt stress was
carried out with an aim to identify most suitable reference genes for the
normalization of gene expression data in rice. After four days of drought
and salinity stress the resistant cultivars showed higher RWC as compared
to the susceptible cultivars. The drought susceptible cultivar, GR 3
showed higher leaf rolling index (LRI) as compared to the resistant cultivar
AAUDR 1. Based on the visual symptoms of salt damage the tolerant
cultivar Dandi scored 3 in the Standard Evaluation System (SES) and
susceptible cultivar GR 11 scored 7, indicating tolerance and susceptible
reactions, respectively.
cDNA preparation was carried out from total RNA of rice seedlings
Description
Keywords
Plant Molecular Biology, Study