“Identification and validation of internal control genes for quantitative gene expression studies in pearl millet under biotic stress (downy mildew infection) and abiotic stress (salt stress

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Date
2012
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Anand Agricultural University, Anand
Abstract
Pearl millet (Pennisetum glauccum (L.) R. Br.) is an annual, cross pollinating, monocot crop belonging to the family Poaceae and sub family Penicedae. It is the sixth most important cereal of the world and has critical value as food security in some of the world’s hottest and driest cultivated areas of Africa and Asia. However, the crop faces constraints to the productivity which includes the prevalence of different biotic and abiotic stresses. The present work was carried out with an aim to identify and select endogenous genes for pearlmillet under biotic stress (downy mildew) and abiotic stress (salinity) and validating constitutively expressed genes in pearlmillet through RT-PCR and analyzed it through geNorm for best stable reference gene
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Plant Biotechnology, Analysis
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