Loading...
Theses (PG)
Browse
Search Results
ThesisItem Open Access STUDIES ON METABOLIC AND HEMATOLOGICAL PROFILES IN CROSSBRED COWS WITH CYTOLOGICAL ENDOMETRITIS(KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2021) AVINASH R.G.; A M KOTRESHThe cytological endometritis (CE) is an inflammation of the endometrium without visible clinical signs significantly impairing the fertility in dairy cows. The study was undertaken to assess the metabolic stress and hematological profile influencing CE in crossbred dairy cows reared in the central dry zone of Karnataka. Apparently healthy crossbred cows in 3-11th week postpartum (WPP) were divided into four groups (each N=8) viz., Group- I as control having crossbred cows at 3-11th WPP estrus, Group- II at 3-5th, Group- III at 6-8th and Group- IV at 8-11 WPP estrus. CE was diagnosed based on cytobrush technique. A significant(p<0.05) increase in concentrations of BHBA and NEFA found in all CE groups compared to control group. There was no significant difference (p<0.05) found in blood glucose, serum total proteins, creatinine, AST, ALT levels in all CE cow groups compared to control group. The BUN, total cholesterol and bilirubin levels were found significantly(p<0.05) higher and calcium and phosphorous values were lower in CE affected cow groups compared to control group. There was no significant (p<0.05) difference in blood parameters such as WBCs, lymphocytes, HB, PCV, MCV, MCH, MCHC, platelet among all CE groups when compared to control group. There was significant (p<0.05) increase in percentage of neutrophils and reduced RBC levels were found in CE affected cows. There was a positive correlation found with respect to NEFA and BHBA with lymphocytes, neutrophils activity in all CE affected cow groups. There was negative correlation found between calcium and phosphorous with lymphocytes, neutrophils activity in all CE cow groups. Key words: Cytological endometritis, Metabolic stress, Hematological profile, Body condition score.ThesisItem Open Access STUDIES ON BIOCHEMICAL, ANTIOXIDANT AND SOMATIC CELL COUNT PROFILE OF SUBCLINICAL MASTITIS IN DAIRY CATTLE WITH OR WITHOUT CO-INFECTIONS /DISORDERS(KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCIES UNIVERSITY, BIDAR, 2019) MOHAMMED KHALEEL ULLA; A.M KOTRESHDetermination of biochemical changes, oxidative stress and variation in somatic cell counts(SCC) in animals with subclinical mastitis and existing coinfections/ disorders or with previous illness were analyzed in the present study. The SCC of 2 X 105 cells/ml of milk was taken as physiological threshold for the detection of subclinical mastitis in this region of study and upper limit was set at 2 X 105 cells/ml of milk and above which the animals were categorized as clinical mastitis. The incidence of subclinical mastitis in 60 lactating cows were categorized as Group M (n=60) of which further grouped as Group FM(Frequent Mastitis),TS(Teat stenosis), RA(Ruminal acidosis), ND(Nonspecific diarrhea), RTI(Respiratory tract infections), RB(Repeat breeders), PP (Plant poisoning), EF (Bovine ephemeral fever) and RD (Rare disorders) based upon the co-infections /disorders or previous illness and Control group C. The increase in concentrations of alkaline phsophotase (ALP), Aspartate amino transferase(AST), blood urea nitrogen (BUN) and total plasma proteins(TPP) were seen in the cows where subclinical mastitis is detected. The antioxidant enzymes like catalase,super oxide dismutase (SOD), Glutathione Peroxidase (GPx) levels were significantly declined and the non enzymatic parameters reduced glutathione (GSH) and malondialdehyde (MDA) are significantly elevated in affected animals than in healthy animals. There is positive correlation between SCC with GSH and MDA however the results of correlation of SCC with other biochemical parameters like ALP, ALT, AST, TPP, BUN, calcium, phosphorous revealed no significant correlation between the healthy animals and the subclinical mastitis affected animals. Keywords: Subclinical mastitis, somatic cell counts, biochemical profile, oxidative stress, lactating cows.ThesisItem Open Access EVALUATION OF DIETARY BETAINE SUPPLEMENTATION ON HEMATO-BIOCHEMICAL PROFILE, MILK YIELD AND MILK COMPOSITION IN DEONI COWS(KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2021-03) SONABAI KONDIBA; SHRIKANT KULKARNIA study was conducted to assess effects of dietary betaine supplementation on haemato-biochemical profile, milk yield and composition, SCC, biochemical characteristics in lactating Deoni cows. Twelve lactating cows were randomly divided into control and treatment group with six in each group. Control group was fed basal diet alone whereas treatment group was supplemented with anhydrous betaine hydrochloride @ 15 g/ day in the diet during the study period of 8 week in addition to basal diet. The average daily milk yield improved significantly (P<0.05) in treatment group from 5th week; milk fat and solids not fat from 3rd week of after supplementing betaine. There was no significant difference in milk lactose level between two groups during the study period. Somatic cell count in the milk declined significantly (P<0.05) in betaine supplemented Deoni cows from 4th week of study. There was no difference between two groups in TEC, Hb, PCV and TLC. Percentages of lymphocytes were significantly higher and that of neutrophils were lower in treatment group as compared to control. The concentration of cholesterol, triglycerides and BUN were significantly (P<0.05)decreased from 4th week and total proteins increased from 2nd week of betaine supplementation in lactating Deoni cows. There were no significant differences in plasma electrolytes in betaine supplemented groups compared to control, although plasma sodium and potassium were numerically higher and chloride levels were numerically lower when compared to control group. It can be concluded that dietary betaine supplementation to lactating Deoni cows enhances milk yield and improves milk composition and udder health.ThesisItem Open Access STUDIES ON METABOLIC GROWTH INDICATORS DURING DIFFERENT STAGES OF GROWTH IN BANNUR FEMALE SHEEP(KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR-585401, 2018-09) VEENA, M. P.; Dr. M. NARAYANA SWAMYThe present study was conducted to ascertain the metabolic growth indicators during different stages of growth in Bannur female sheep maintained at Bandur Breeding Farm, Danagur Village, Malavalli Taluk, Mandya District. Twenty four female sheep divided into four groups with six animals in each group such as Group I (>3 to 5 months), Group II (>5 to 7 months), Group III (>7 to 9 months) and Group IV (>9 to 11 months) were utilized in the present study. The blood samples were collected from jugular vein at fortnightly intervals for two months (15th, 30th, 45th and 60th day). Each time about 5 to 8 ml of blood was collected in clot activated vacutainer. A drop of blood during collection was immediately utilized for estimation of blood glucose and β- hydroxybutyrate (BHB). Serum samples were immediately utilized for determination of alkaline phosphatase (ALP) and creatine kinase (CK) activity. In the present study it was found that the blood glucose, serum total protein, serum total cholesterol, leptin, insulin, estradiol, T3 and potassium levels were increased significantly in advance stages compared to early stage of growth. However, the BHB levels were constant in different age groups. T4 levels increased in advance growth period, calcium and phosphorus levels did not differ with respect to age. Growth hormone increased in the early stage, but ALP and CK activity reduced as the age advanced. Body weight increased from beginning to the completion of the study. It was concluded that the levels of blood glucose, BHB, leptin, insulin, GH, potassium, ALP, CK and body weight gain could be used as physiological indicators of growth in Bannur female sheep to ascertain growth and the same can be extrapolated to other breeds of sheep. Key words: Metabolic growth indicators,ThesisItem Open Access BIOCHEMICAL, PHYSICO-CHEMICAL AND PROTEOMIC PROFILING OF DIFFERENT SKELETAL MUSCLES OF SHEEP(KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR- 585 401, 2019-08) GANGADHAR KAPASE; Dr. SHRIKANT KULKARNIThe present experiment was designed to study the biochemical, physico-chemical and proteomic profile of different skeletal muscles viz., Vastus lateralis (VL), Gluteo biceps femoris (GBF), Gluteo medius (GM), Longissimus thoracis et lumborum (LTL), Psoas major (PM) and Semitendinosus (ST) and also to assess impact of postmortem aging on these parameters in sheep. Muscles were evaluated for various biochemical parameters viz., myoglobin, % metmyoglobin, R value, protein extractability, collagen content and solubility, TBARS ; physico-chemical parameters viz., pH, WHC, drip loss, MFI, muscle fiber diameter, WBSF and colour indices in fresh samples (day 0), 5th, 10th and 15th day of postmortem period using standard procedures. The SDS-PAGE followed by MALDI TOF MS/MS technique was employed in proteomic study. There were significant (p<0.05) differences between the skeletal muscles in various biochemical and physico-chemical parameters in fresh muscle samples (day 0). The R value, total protein extractability, sarcoplasmic protein extractability, myofibrillar protein extractability, collagen content and solubility, TBARS, pH, WHC, MFI, colour indices (L*, a* and b*) increased significantly (p<0.05) whereas myoglobin content and WBSF values decreased significantly (p<0.05) as the postmortem period advanced. Proteomic studies revealed the protein degradation in muscles due to postmortem aging. The proteins identified by MALDI TOF MS/ MS are Adenylate kinase isoenzyme, Intracellular hyaluronan-binding protein, Lymphocyte antigen 86, Actin, Integrin alpha-6, and PDZ and LIM domain protein 3. The significant differences in biochemical, physico-chemical and proteomic profile during different postmortem period indicated that many biochemical and structural changes occur during postmortem period that convert muscle to meatThesisItem Open Access SERUM AND MILK COMPONENTS IN AMRIT MAHAL, HOLSTEIN FRIESIAN CROSSBRED AND JERSEY CROSSBRED COWS(KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2018-09) PRAVEEN, V.; Dr. M. NARAYANA SWAMYThe present study was undertaken in Amrit Mahal (Group I), Holstein Friesian crossbred (Group II) and Jersey crossbred (Group III) cows at third lactation to assess blood metabolic profile and milk components profile. A total of eighteen cows with six cows in each group were selected randomly from different dairy farms. The blood and milk samples were collected during fourth, eighth and twelfth weeks of lactation. The serum samples were subjected for the estimation of the levels of glucose, total protein, albumin, globulin, A/G ratio, urea nitrogen, total cholesterol, triglycerides, calcium, phosphorus, magnesium, sodium, potassium and chloride. The milk components such as percentage of lactose, fat, proteins and solids-not-fat were measured using ultrasonic lactoscan milk analyser. The pH of milk was measured using digital pH meter. The daily milk yield was also recorded. Serum components such as glucose, total protein and globulin were significantly higher during fourth week compared to twelfth week of lactation in Amrit Mahal cows. The serum lipid and mineral profile were higher in Amrit Mahal cows compared to crossbred cows. The milk profile of different parameters such as lactose, protein, fat and SNF were significantly higher in Amrit Mahal cows compared to crossbred cows. Whereas, milk yield and pH was higher in crossbred cows compared to Amrit Mahal cows. It was concluded that the levels of some of the serum and milk components were higher in Amrit Mahal cows compared to Holstein Friesian crossbred and Jersey crossbred cows. Therefore, Amrit Mahal cows should be conserved for further cross breeding programmes.ThesisItem Open Access SERUM ENZYMATIC AND LIPID PROFILE IN HOLSTEIN FRIESIAN CROSS BRED COWS WITH LOW SOLIDS-NOT-FAT SYNDROME(KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2017-09) MANEESHA, H. S.; Dr. M. NARAYANA SWAMYThe present study was conducted to estimate the enzymatic activities, lipid and blood metabolic profile in Holstein Friesian crossbred cows at 3rd and 5th lactation. Twenty four cows were divided into four groups with each group comprising of six animals based on lactation number and the milk SNF content. Group I, II, III and IV comprised of cows of 3rd lactation with normal milk SNF content, cows of 3rd lactation with low milk SNF content, cows of 5th lactation with normal milk SNF content and cows of 5th lactation with low milk SNF content, respectively. The blood and serum samples were collected from each animal at 4th and 8th week of lactation that were analyzed for blood glucose, blood β-hydroxy butyric acid, serum enzyme activities such as AST, ALT, ALP and LDH, lipid profile components and total serum protein. At 3rd lactation, significantly (P<0.05) higher ALP and HDL-C was noted in normal SNF cows compared to low SNF cows. Significantly lower activity of ALT was found in normal SNF cows at both 4th and 8th week of 3 rd lactation. At 5th lactation, blood glucose was significantly higher in normal SNF cows compared to low SNF cows at both 4th and 8th week of lactation. AST, LDL-C, VLDL-C and BHBA did not vary significantly between groups. LDH was significantly higher at 4 th week and cholesterol was significantly higher at 8 th week of 3rd lactation. Triglycerides and total serum protein were significantly higher in normal SNF cows at 8th week of both the lactations. It was concluded that the decreased blood glucose level in low SNF groups could be a reason for low SNF syndrome in HF crossbred cows.ThesisItem Open Access STUDIES ON HEMATO-BIOCHEMICAL PROFILE AND IMMUNE RESPONSIVENESS TO VACCINATION AGAINST FOOT AND MOUTH DISEASE IN MALNAD GIDDA CATTLE(KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2017-07) HEMANTH GOWDA, K.; Dr. M. NARAYANA SWAMYThe present study was conducted to determine the reference values for hematological parameters, biochemical parameters, female reproductive hormones in Malnad Gidda cattle and the physiogenomic aspect such as genetic polymorphism at MHC DRB3.2 loci and cellular immune responsiveness to FMD vaccination in Malnad Gidda compared to Hallikar X HF crossbred cattle. Significantly lower values were observed in young age groups of both sexes for TEC, hemoglobin, PCV, ESR, TLC, eosinophil per cent, except MCV which was significantly higher in young age groups of both sexes. However, No significant difference was observed for MCH, MCHC, and the percentages of neutrophil, basophil, monocyte and lymphocyte. The levels of different biochemical parameters such as the levels of serum total cholesterol, serum triglycerides, HDL cholesterol, LDL cholesterol and VLDL cholesterol, serum total protein, serum albumin, BUN, creatinine, β-hydroxy butyrate, blood glucose and the activities of AST and ALT did not differ significantly among groups. Significant difference was not observed between winter and summer seasons for any of the hematological and biochemical profile taken up for the study among different groups. The reproductive hormones such as 17β-estradiol and progesterone showed increasing and decreasing pattern respectively, during estrous cycle in Malnad Gidda cattle similar to other breeds of cattle. The two allelic variants MHC DRB3.2*117 and *219 present between Malnad Gidda and Holstein Friesian crossbred cattle could be responsible for better cellular immune responsiveness with significantly higher CD4 lymphocytes population to FMD vaccination in Malnad Gidda cattle compared to Hallikar X HF crossbred cattle.ThesisItem Open Access STUDIES ON URINARY COMPONENTS IN MALNAD GIDDA, JERSEY CROSS AND HOLSTEIN FRIESIAN CROSS BRED COWS(Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2014) VEENA, M.VIn India, drinking of cow urine as health drink has been practiced for thousands of years.
