BIOCHEMICAL, PHYSICO-CHEMICAL AND PROTEOMIC PROFILING OF DIFFERENT SKELETAL MUSCLES OF SHEEP
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Date
2019-08
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KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR- 585 401
Abstract
The present experiment was designed to study the biochemical, physico-chemical
and proteomic profile of different skeletal muscles viz., Vastus lateralis (VL), Gluteo
biceps femoris (GBF), Gluteo medius (GM), Longissimus thoracis et lumborum (LTL),
Psoas major (PM) and Semitendinosus (ST) and also to assess impact of postmortem aging
on these parameters in sheep. Muscles were evaluated for various biochemical parameters
viz., myoglobin, % metmyoglobin, R value, protein extractability, collagen content and
solubility, TBARS ; physico-chemical parameters viz., pH, WHC, drip loss, MFI, muscle
fiber diameter, WBSF and colour indices in fresh samples (day 0), 5th, 10th and 15th day of
postmortem period using standard procedures. The SDS-PAGE followed by MALDI TOF
MS/MS technique was employed in proteomic study. There were significant (p<0.05)
differences between the skeletal muscles in various biochemical and physico-chemical
parameters in fresh muscle samples (day 0). The R value, total protein extractability,
sarcoplasmic protein extractability, myofibrillar protein extractability, collagen content
and solubility, TBARS, pH, WHC, MFI, colour indices (L*, a* and b*) increased
significantly (p<0.05) whereas myoglobin content and WBSF values decreased
significantly (p<0.05) as the postmortem period advanced. Proteomic studies revealed the
protein degradation in muscles due to postmortem aging. The proteins identified by
MALDI TOF MS/ MS are Adenylate kinase isoenzyme, Intracellular hyaluronan-binding
protein, Lymphocyte antigen 86, Actin, Integrin alpha-6, and PDZ and LIM domain protein
3. The significant differences in biochemical, physico-chemical and proteomic profile
during different postmortem period indicated that many biochemical and structural changes
occur during postmortem period that convert muscle to meat