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2013 - 201932020 - 20221
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Author: Arya, Mamta × End date: 2022 × Type: Thesis ×
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    ThesisItemOpen Access
    Genetic diversity, phytochemical and anticarcinogenic studies in cichorium intybus from Himalayan elevations of Uttarakhand
    (G. B. Pant University of Agriculture and Technology, Pantnagar, 2022-08) Arya, Mamta; Gohar Taj
    Chicory (Cichorium intybus L.) comprise of intense therapeutic and medicinal properties due to presence of phytochemical and secondary metabolites. It is often used as a medication in India to treat a number of illnesses such as fever, diarrhea, spleen enlargement, gout, and rheumatism. The purpose of this study was to determine the genetic diversity of chicory from Himalayan regions of Uttarakhand using SSR markers. So, it will be entrancing to know about the genetic diversity of chicory and if any genotypes are deficient in medicinal content then it could be enhanced through various molecular interventions. The cluster analysis of 100 genotypes grouped it into two clusters A and B, in which cluster A comprised of 94 genotypes. The other B cluster consists of 6 genotypes. Additionally, to investigate the genetic constitution of diverse 100 chicory genotypes genotyping by sequencing was performed, population structure and genetic diversity analysis were also done. Cluster analysis using neighbor joining method grouped the population into 3 clusters ‘A’, ‘B’ and ‘C’ depending on their geographical location. The main cluster ‘C’ consists of 61 genotypes, cluster B comprised of 28 genotypes and cluster A comprised of 11 genotypes. From GBS technique thousands of SNPs identified were used for the association mapping to recognize authentic markers linked to the agro-morphological traits of Cichorium intybus plant. There were 178 SNPs markers showed associations with the eight agromorphological traits at significant threshold level. Further, to study the difference in the phytochemical constituents quantity of chicory, qualitative and quantitative analysis were performed among 10 genotypes and identified that genotypes showed considerable amount of variation. Lactucine the phytochemical constituents of chicory also have anti-cancer properties and can treat various types of cancers such as human leukemia cancer. In present investigation, lactucine and its derivatives are docked with the apoptosis inducing and cell cycle progression proteins for the prediction of its anticancer effect. Among 17 isoforms of lactucine, Lactucin 15- oxalate has shown highest binding affinity with target protein with appropriate Glide and Dock score. These in silico results are valuable to proceed for the in vitro and in vivo studies related toanti-cancer role of lactucin 15-oxalate. Therefore, chicory plant has high in its diversity can be considered as a high value herbs for the medicinal purposes and even its phytochemical content are also enhancing nutritional values. The identified SNPs with the agro-morphological trait indicate its high rate of association which is useful in improving the medicinal properties. Even, due to anticancer effect of lactucinee can be used for treatment of leukemia cancer.
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    ThesisItemOpen Access
    Extraction and characterization of oak tussar sericin and process optimization for finishing of cotton fabric
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2019-12) Arya, Mamta; Goel, Alka
    The growing ecological consciousness demands for better waste management practices. Thus in the present study an attempt was made to extract sericin, a waste from silk industry .It the present study that sericin was successfully extracted from the degummed liquor of oak tussar silk cocoon. Sericin yield percent was 8.3 at optimum liquor ethanol ratio i.e. 3:1 and incubation time 6 hours. Further FTIR, molecular weight, nitrogen percent, protein content, solubility and amino acid profiling was done and it was found that extracted sericin has amide I, amide II and amide III peaks which confirmed the presence of C=O (1500-1600cm-1) and N-H groups (1400 cm-1). Molecular weight of oak tussar sericin ranged from 15kda to 250kda and nitrogen percent was 2.5%. Various amino acids were found namely Serine, Aspargine, Proline, Threonine, Cystine, Glycine, Alanine, Methionine, Phenylalanine, Tryptophan, Glutamic acid and Aspartic acid whereas serine percentage was highest (22%) among all the amino acids. Cotton fabric was finished with extracted oak tussar sericin. Box-Behnken Design of Response Surface Methodology was used for optimization of finishing variables. After the analysis of linear, interactive and quadratic model terms the optimized recipe for finishing was consisted of 51gpl sericin concentration, 2% citric acid, 60˚C temperature, 60 minutes time and 1:20 M L Ratio. Finished fabric was then tested for physical, comfort and functional properties. It was found that durability of finish was good. Breaking strength of finished fabric was 21 kgs in warp direction and 17.8 kg in weft direction. Elongation of finished fabric was 23% in warp and 23.8 % in weft direction. Thickness (from 0.23mm to0.25mm), fabric weight (from 98 g/m2 to 101 g/m2) and bending length (from 1.4 cm to 1.6 cm) of finished fabric were increased. Crease recovery angle of finished fabric was increased significantly from 78˚ to 102˚ in warp and 84˚ to 112˚ in weft direction which inferred that creasing of finished fabric was decreased. Thermal resistance of fabric was improved as clo value of finished fabric (0.21clo) was more than the control fabric (0.04). Air permeability and wicking of finished fabric was decreased whereas water absorption of finished fabric was improved. Finished fabric exhibited improved antimicrobial property with different concentration (35gpl, 50gpl and 65gpl) of sericin. Highest zone of inhibition i.e. 6mm against E.coli and 7mm against S. aureus was observed with the highest concentration of sericin. Better UPF property was also observed in the finished fabric as UPF value was increased from 8.6 to 15.8. Further dyeing of finished fabric was done with synthetic direct dye and Butea monosperma natural dye. In case of direct dyes it was found that when dyeing was done on the sericin finish, fabric colour strength was slightly decreased whereas colour strength was increased when dyeing was done in the fabric finished with sericin and dyed with Butea monosperma. Overall fastness of both the dyes was improved with the finish application. The cost of sericin finished fabric was found Rs 650 per meter. The extracted sericin can be potentially used as biopolymer in textile dyeing industry. The developed finished fabric can be utilized in apparel sector and in medical textiles as bed linen, surgical gowns, uniforms, wipes etc.
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    ThesisItemOpen Access
    Value addition of dhaincha (Sesbania aculeata) fibres through dyeing with synthetic and natural dyes
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2013-07) Arya, Mamta; Anita Rani
    The growing ecological consciousness and limited land availability for cultivation of fibre yielding crops have pressed researchers to explore under-utilized and new natural resources that could offer textile fibres for numerous end uses both technical and aesthetic. The hills of Himalayas and Tarai region of Kumaon are endowed with indigenous flora rich in natural fibres due to favourable climatic conditions. Some of these plants are bhimal, bhindi, bhang, rambans, bichu grass, shahtoot, timla, banana and oak used by the local masses for fibre extraction and its usage for manufacturing household items. One such unconventional plant source of textile fibre is dhaincha. Dhaincha is a green manure crop used prior to paddy cultivation and is also one of the valuable medicinal herbs. Botanically, this plant is known as Sesbania aculeata and by common names as danchi, dhaincha, dunchi, danicha. This plant belongs to leguminacea family. This harsh, coarse and shiny fibre has potential to find use in the different sectors. The present study was, therefore, planned to explore the use of dhaincha fibre in home textiles. Value addition of dhaincha fibres were done through dyeing with five synthetic dyes namely reactive, sulphur, vat, direct, and azoic and five natural dyes namely, Rubia cardifolia, Acacia catechu, Punica granatum, Indigofera tinctoria, and Curcuma longa. Optimization of M: L ratio and dyeing time was done prior to application of dyes on the fibres. Further the effect of dyes on physical properties of dhaincha fibre was assessed. Also the fastness of dyed dhaincha fibre was tested against washing, rubbing and light. Finally non woven fabric was prepared by needle punching method for product development It was found that the fibres could be dyed easily with natural and synthetic dyes. The dyed fibres exhibited high to medium colour strength. The fastness ranged from fair to very good against washing; very good to excellent against rubbing and poor to good against light. The dyeing of fibres affected the physical properties too. The moisture regain of the fibres improved effectively on dyeing with both the types of dyes. Non woven fabric was prepared by needle punching loom and fabric was utilized to make hand fan, magazine holder and table mat to showcase the possible use of dhaincha non woven for home textiles and travel utilities in vehicles.
  • Thumbnail Image
    ThesisItemOpen Access
    Value addition of dhaincha (Sesbania aculeata) fibres through dyeing with synthetic and natural dyes
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2013-07) Arya, Mamta; Anita Rani
    The growing ecological consciousness and limited land availability for cultivation of fibre yielding crops have pressed researchers to explore under-utilized and new natural resources that could offer textile fibres for numerous end uses both technical and aesthetic. The hills of Himalayas and Tarai region of Kumaon are endowed with indigenous flora rich in natural fibres due to favourable climatic conditions. Some of these plants are bhimal, bhindi, bhang, rambans, bichu grass, shahtoot, timla, banana and oak used by the local masses for fibre extraction and its usage for manufacturing household items. One such unconventional plant source of textile fibre is dhaincha. Dhaincha is a green manure crop used prior to paddy cultivation and is also one of the valuable medicinal herbs. Botanically, this plant is known as Sesbania aculeata and by common names as danchi, dhaincha, dunchi, danicha. This plant belongs to leguminacea family. This harsh, coarse and shiny fibre has potential to find use in the different sectors. The present study was, therefore, planned to explore the use of dhaincha fibre in home textiles. Value addition of dhaincha fibres were done through dyeing with five synthetic dyes namely reactive, sulphur, vat, direct, and azoic and five natural dyes namely, Rubia cardifolia, Acacia catechu, Punica granatum, Indigofera tinctoria, and Curcuma longa. Optimization of M: L ratio and dyeing time was done prior to application of dyes on the fibres. Further the effect of dyes on physical properties of dhaincha fibre was assessed. Also the fastness of dyed dhaincha fibre was tested against washing, rubbing and light. Finally non woven fabric was prepared by needle punching method for product development It was found that the fibres could be dyed easily with natural and synthetic dyes. The dyed fibres exhibited high to medium colour strength. The fastness ranged from fair to very good against washing; very good to excellent against rubbing and poor to good against light. The dyeing of fibres affected the physical properties too. The moisture regain of the fibres improved effectively on dyeing with both the types of dyes. Non woven fabric was prepared by needle punching loom and fabric was utilized to make hand fan, magazine holder and table mat to showcase the possible use of dhaincha non woven for home textiles and travel utilities in vehicles.