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Theses

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2016 - 20192
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Subject: Veterinary Surgery and Radiology × Author: Arun Kumar × End date: 2021 × Type: Thesis ×
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    ThesisItemOpen Access
    Apoptic effects of vincristine sulphate and cisplatin scaffolds on HeLa cell line and their clinical efficacy on canine transmissible venereal tumors
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2019-06) Arun Kumar; Jadon, N.S.
    The present study was undertaken to evaluate the effects of vincristine sulphate, cisplatin and their scaffolds on canine transmissible venereal tumour in twenty four sexually mature adult dogs affected with naturally occurring canine transmissible venereal tumour (CTVT). The animals were randomly divided into four groups (n=6) and subjected to administration of different oncolytic drugs and drugs scaffolds. The animals of group A were administered vincristine sulphate @ 0.025 mg/kg intravenously once in a week for four consecutive weeks and animals of group B were administered cisplatin @ 2.14 mg/kg intravenously and repeated after 21 days. The animals of group C and D were subjected to the administration of scaffolds of vincristine sulphate @ 0.025 mg/kg intravenously once in a week for four consecutive weeks and scaffolds of cisplatin @ 2.14mg/kg intravenously and repeated after 21 days respectively. Preparation and characterization of hydrogel scaffolds were consisting of FTIR measurements, ultrastructure studies and electrochemical analysis. The oncolytic potential of the different chemotherapeutic agent (vincristine sulphate, cisplatin and their scaffolds) was evaluated on the basis of physical and cytological parameters, histopathological studies, haemato-biochemical parameters(Hb, PCV, TLC, DLC and platelets, total protein, glucose, BUN, creatinine, ALT, ASTand GGT) and apoptotic effect on HeLa cell line. Genomic DNA from HeLa cells was isolated and subjected to electrophoresis in agarose gel (1.8%) and 1kb DNA ladder. DNA fragments were visualized under a UV trans-illuminator and compared with a standard marker. Lane C showed no fragmented DNA, however, 20 μg/ml vincristine and 20 μg/ml cisplatin showed fragmented DNA in the form of ladder 1 and 3 after 24 h. Vincristine scaffolds and cisplatin scaffolds showed mild DNA fragment in lane 2 and 4. On the basis of parameter observed in this study, it is concluded that the early and best regression of the CTVT was observed in the animals treated with vincristine scaffolds. Cisplatin regressed the CTVT masses upto some extent; however, cisplatin scaffolds are moderately effective when it is used in appropriate dose. Vincristine alone is effective drug for the treatment of CTVT even in metastatic conditions, however the vincristine scaffolds are more effective as it has early regression of tumour as compare to vincristine alone. This may be due to decreasing the side effects caused in healthy cells. These vincristine scaffolds may be used safely by field veterinarian for the treatment of TVT in canines.
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    ThesisItemOpen Access
    Studies on effect of dexmedetomidine, ketamine and etomidate anaesthesia in dogs
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2016-07) Arun Kumar; Kandpal, Manjul
    The study was conducted on twenty four clinically healthy dogs of either sex, divided into four groups with the objective to evaluate a safe anaesthetic protocol that could lead to fast and smoother recovery. Ketamine and etomidate alone or in combination with dexmedetomidine were used for this purpose. In group 1 ketamine was administered intravenously @ 10 mg/kg body weight. In group 2, dexmedetomidine was administered @ 10 µg/kg body weight, intravenously followed by ketamine to the effect (3.17±0.15 mg/kg). In group 3, etomidate 3 mg/kg body weight intravenous was administered and in group 4 dexmedetomidine was administered @ 10 µg/kg body weight, intravenously and followed by etomidate to the effect (1.32±0.03 mg/kg). The efficacy of different anaesthetic protocol was accessed by determining various clinicophysiological, cardiopulmonary and haematobiochemical parameters before and after anaesthesia. Dexmedetomidine prior to administration of ketamine or etomidate results in quicker induction, better analgesia for more duration and better muscle relaxation as compared to use of ketamine and etomidate alone. Heart rate increased significantly in group 1 and 2 after anaesthesia. In group 3 non-significant increased and in group 4 significant decrease in heart rate was observed. Respiration rate decreased significantly in all four groups except in group 1. Rectal temperature was non significantly decreased in all groups. Haemoglobin level was decreased non significantly in the animals of all groups. The PCV level was decrease significantly in group 1, 2 and 3 whereas in group 4 PCV level decrease non significantly. The TEC level was decreased significantly in group 1, 2 and 4 whereas it decreases non significantly in group 3. Non significant change were observed in TLC level. In group 4 a non significant and in other 3 groups significant increase in glucose level was observed. The creatinine level increase significantly in group 3 and non significantly in group 1, 2 and 4. Blood urea nitrogen showed a non-significant increase in group 2 and 4 and significant increased in group 1 however it decreases non significantly in group 3. The total proteins showed a non-significant decrease in the animals of all groups. ALT and AST level was non significantly increase in all the groups. Insignificant change were also observed in serum electrolyte level.