Apoptic effects of vincristine sulphate and cisplatin scaffolds on HeLa cell line and their clinical efficacy on canine transmissible venereal tumors
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Date
2019-06
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G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
The present study was undertaken to evaluate the effects of vincristine sulphate, cisplatin and their scaffolds on canine transmissible venereal tumour in twenty four sexually mature adult dogs affected with naturally occurring canine transmissible venereal tumour (CTVT). The animals were randomly divided into four groups (n=6) and subjected to administration of different oncolytic drugs and drugs scaffolds. The animals of group A were administered vincristine sulphate @ 0.025 mg/kg intravenously once in a week for four consecutive weeks and animals of group B were administered cisplatin @ 2.14 mg/kg intravenously and repeated after 21 days. The animals of group C and D were subjected to the administration of scaffolds of vincristine sulphate @ 0.025 mg/kg intravenously once in a week for four consecutive weeks and scaffolds of cisplatin @ 2.14mg/kg intravenously and repeated after 21 days respectively. Preparation and characterization of hydrogel scaffolds were consisting of FTIR measurements, ultrastructure studies and electrochemical analysis. The oncolytic potential of the different chemotherapeutic agent (vincristine sulphate, cisplatin and their scaffolds) was evaluated on the basis of physical and cytological parameters, histopathological studies, haemato-biochemical parameters(Hb, PCV, TLC, DLC and platelets, total protein, glucose, BUN, creatinine, ALT, ASTand GGT) and apoptotic effect on HeLa cell line. Genomic DNA from HeLa cells was isolated and subjected to electrophoresis in agarose gel (1.8%) and 1kb DNA ladder. DNA fragments were visualized under a UV trans-illuminator and compared with a standard marker. Lane C showed no fragmented DNA, however, 20 μg/ml vincristine and 20 μg/ml cisplatin showed fragmented DNA in the form of ladder 1 and 3 after 24 h. Vincristine scaffolds and cisplatin scaffolds showed mild DNA fragment in lane 2 and 4. On the basis of parameter observed in this study, it is concluded that the early and best regression of the CTVT was observed in the animals treated with vincristine scaffolds. Cisplatin regressed the CTVT masses upto some extent; however, cisplatin scaffolds are moderately effective when it is used in appropriate dose. Vincristine alone is effective drug for the treatment of CTVT even in metastatic conditions, however the vincristine scaffolds are more effective as it has early regression of tumour as compare to vincristine alone. This may be due to decreasing the side effects caused in healthy cells. These vincristine scaffolds may be used safely by field veterinarian for the treatment of TVT in canines.
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