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2012 - 201754
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Subject: Genetics and Plant Breeding × End date: 2017 × Start date: 2010 × Thesis Type: M.Sc ×
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Now showing 1 - 9 of 54
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    ThesisItemOpen Access
    Genotype × environment interaction for yield and related traits in some advance lines of pigeonpea (Cajanus cajan (L.) Millsp)
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2017-12) Pal, Deepak; Verma, S.K.
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    ThesisItemOpen Access
    Diversity analysis for quantitative traits, biochemical profiling and screening of MYMV in blackgram (Vigna mungo (L.) Hepper) germplasm collected from Uttarakhand hills
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2013-06) Gupta, Richa; Arora, Anju
    Blackgram (Vigna mungo (L.) Hepper, 2n = 2x = 22) commonly known as urdbean or mash is an important pulse crop of India. The present investigation was carried out at N.E. Borlaug Crop Research Centre, Pantnagar, during kharif, 2011 and 2012 with 54 genotypes (including 50 test lines and 4 check varieties) of blackgram for ten quantitative traits with the objectives to study genetic divergence, character association for yield and its components, quantification of seed storage protein and identification of genotypes with high protein content, polymorphism in genotypes using SDS – PAGE and to identify resistant germplasm source for MYMV disease. Mean squares due to genotypes were highly significant for plant height, number of clusters per plant, number of pods per plant, seeds per pod, 100 seed weight and grain yield per plant while significant for number of pods per cluster. The highest yield was recorded as 10.55 g/plant for genotype GP 2011-15 followed by 10.30 g/plant for GP 2011- 64. Character association analysis revealed that yield per plant showed highly significant and positive correlation with all the characters except days to 50% flowering and 100 seed weight. Based on non-hierarchical euclidean cluster analysis, 54 genotypes were grouped into nine non-overlapping clusters. The cluster V comprised of maximum number of genotypes (11) whereas, cluster VII consisted of minimum number of genotypes (2). Highest inter cluster distance was observed between the genotypes of clusters IV and VII followed by clusters IV and VI. Six genotypes viz., GP 2011-15, GP 2011-448, GP 2011-152, GP 2011-64, IS-1 and RS 241 were identified as divergent and desirable for particular characters. Genotype GP 2011-15 was selected for number of clusters/plant, number of pods/plant and yield/plant, IS-1 for seeds/pod, GP 2011-448 was selected for plant height and 100 seed weight, GP 2011-152 for number of branches/plant, genotype GP 2011-64 for number of pods/cluster and pod length and RS 241 was selected for days to 50% flowering. Among genotypes evaluated some of them could be a good source of seed storage protein. In blackgram seeds protein ranged from 20.22 to 23.06%. PCPGR 8027 and PCPGR 8022 contained highest amount of total protein i.e. 23.06% and 23.03% respectively. The genotypes were grouped on the basis of SDS-PAGE analysis. The dissimilarity coefficient ranged between 0 - 0.80. A dendrogram based on the UPGMA analysis grouped 54 genotypes into 2 main clusters, cluster A and cluster B. Cluster A consisted of 26 genotypes and the remaining 28 were grouped in cluster B. Genotypes were also screened against MYMV disease. Six genotypes viz., EK 91, IS-1, Urd 83, GP 2011-15, GP 2011-64 and PCPGR 8026 were found immune against MYMV. Three genotypes GP 2011-15, GP 2011-64 and PCPGR 8026 were found agronomically good and immune against MYMV disease, whereas, PCPGR 8026 was found early maturing and contained high seed storage protein.
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    ThesisItemOpen Access
    Genetic analysis of grain yield and its components with field and molecular screening for yellow rust resistance in bread wheat [Triticum aestivum L. em Thell]
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2013-07) Mohammed Talha; Swati
    Research work on combining ability analysis and gene action for yield contributing traits with field and molecular screening for yellow rust resistance of fifteen wheat genotypes and their respective crosses was carried out using Line x tester analysis during Rabi 2012-2013 at Norman E. Borlaug Crop Research Centre of Govind Ballabh Pant University of Agriculture and Technology, Pantnagar. Randomized Block Design with three replications was used to study combining ability, gene action and heterosis for days to 75% heading, days to maturity, plant height (cm), peduncle length (cm), productive tillers per plant, flag leaf area (cm2), spike length (cm), number of spikelets per spike, number of grains per spike, weight of grain per spike (g), 1000 grain weight (g), biological yield per plant (g), grain yield per plant (g), harvest index (%) and AUDPC for yellow rust. Field and molecular screening of genotypes was undertaken to classify the genotypes according to disease reaction and to detect presence of major Yr genes. The relative estimates of variance due to sca were higher than the variance due to gca for most of the traits studied indicating the predominance of non- additive gene action whereas for plant height, peduncle length, flag leaf area and AUDPC additive genetic mechanism are more important. Among the tester, DPW 621-50 can be ranked as the best tester and among lines UP2596, PBW 639 and PBW 658 can be considered as the best lines as these combine well for the highest number of yield contributing characters as well as yellow rust resistance. The cross WH1100 x PBW550 was found as good cross combination for maximum number of traits. Some best crosses based on highest sca effects in desirable direction were WH1100 X FLW21 and KO307 X PBW550 for earliness, HD3065 X PBW550 for reduced plant height, UP 2596 x FLW 21 for productive tillers per plant, WH 1100 x PBW 550 for grain yield per plant, WH 1100 x PBW 550 and KO 307 x DPW 621-50 for harvest index and AUDPC, DBW 71 x FLW 21 for flag leaf area, KO 307 x PBW 550 for peduncle length, HPW 211 x DPW 621-50 for number of grains per spike and spike length and DBW 71 x PBW 550 for 1000 grain weight. Eight crosses viz. HD 3059 x DPW 621-50, WH 1100 x FLW 21, UP 2596 x FLW 21, UP 2596 x PBW 550, HPW 211 x DPW 621-50, PBW 644 x DPW 621-50, UP 2596 x FLW 21 and KO 307 x DPW 621-50 had highest significant sca effects in desirable direction as well as highest per se performance for peduncle length, days to 75% heading, productive tillers per plant, number of spikelets per spike, number of grains per spike, weight of grain per spike, biological yield per plant and AUDPC, respectively. It is advocated that the good combiner genotypes could be used in crossing programme and the crosses having significant sca effects should be tested over the location and years before their exploitation. The best cross for grain yield on the basis of high estimates of standard heterosis is KO 307 x FLW 21. On the basis of field screening, all genotypes were classified in three different categories i.e., resistance, intermediate and susceptible. Molecular analysis using linked SSR primers Xwmc175 and Xpsp3000 revealed likely presence of Yr5 gene in PBW639 and Yr10 gene in HD3065, HPW211 and WH1100. These four genotypes also showed resistance response in the field under artificial inoculated conditions. So after further confirmation, these genotypes can be used as donor parents for above mentioned yellow rust resistance genes.
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    ThesisItemOpen Access
    Evaluation of elite germplasm for identification of potential restorers and maintainers in rice [Oryza sativa L.]
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2014-07) Bisht, Meenakshi; Nautiyal, M.K.
    The present investigation was carried out during kharif 2012 at the Norman E Borlaug Crop Research Centre, G.B. pant University of Agriculture and Technology. The study compromised of thirty-one elite lines, three testers (IR 58025A, Pusa 6A and Pant CMS 2A), one standard check (Pant Dhan 12) and ninety-three F1 hybrids. All the material was planted in Randomized Complete Block Design (RCBD) with two replications following line x tester mating design. The objectives of the present study were (i).To estimate the combining ability of CMS lines and crosses, (ii).To estimate the nature and magnitude of heterosis, and (iii). To identify superior maintainers and restorers for development of potential CMS lines and hybrids. Analysis of variance for combining ability revealed importance of non-additive gene action in the expression of various characters. The results indicated that the NDR 1425 (L5), CR 2648-6-3-2 (L7), UPR 2642-31-1-1 (L22) and KPH 371(L31) were the best combiners among lines as evident from significant GCA effects for high number of yield contributing characters and some parents showing maximum per se performance were also the best general combiner for the characters i.e. Pusa 1592-06-5-2 (L20) for panicle length, NDR 1425 (L5) and Pusa 1509-03-1-7-2 (L18) for 1000 grain weight, R 1530-1546-1-418-1 (L3) for filled grains per panicle, IRBBN 90 (L27) for unfilled grains per panicle. Moderate to high degree of heterobeltiosis and standard heterosis were recorded for grain yield per plant and most of its components. The per cent standard heterosis, for grain yield ranged between -86.397 to 33.272 per cent, suggesting good scope for identifying heterotic crosses for exploitation in optimum fertility conditions. The best heterotic hybrids, UPR 3729F6-2-4-4 x IR 58025 A (L23 x T1), MTU 1121 x Pusa 6A (L15 x T2), R 1530-1546-1-418-1 x Pusa 6A (L3 x T2) and UPR 3425-11-1-1 x IR 58025 A (L1 X T1) in normal conditions expressing higher SCA effects and per se performance, also involved good/ good combining parents into them. These crosses may be suggested for straight use in hybrid breeding. On the basis of spikelet fertility percent, lines were grouped as maintainer, restorer and partial restorer/maintainer lines. Among thirty one lines, none of the lines behave as maintainer and restorer with all the three CMS lines (IR 58025 A, Pusa 6A and Pant CMS 2A), six lines viz . , L4, L9, L10, L14, L15 and L25 behave as maintainers with both IR 58025 A and Pusa 6A CMS lines and rest behave like partial restorer/ maintainer lines.
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    ThesisItemOpen Access
    Studies on variability, path coefficient and character association analysis of morphological and quality traits in finger millet [Eleusine coracana (L.) Gaertn.] germplasm
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2012-08) Yesu Babu, Kandlagunta; Pandey, P.K.
    The present investigation was under taken at Breeder Seed Production centre, GBPUA&T, Pantnagar, Uttarakhand during Kharif 2011. The study was aimed (1) To classify the germplasm lines based on scoring for various qualitative morphological traits, (2) to estimate the amount of genetic variability, heritability and expected genetic advance under selection, (3) to estimate intercharacter-correlation coefficients and direct and indirect effects of various component traits on grain yield, (4) to estimate the amount of protein content in grains. The observations were recorded on 17 quantitative characters and 16 qualitative characters. The analysis of variance revealed that there is highly significant difference among all 33 treatments for all the characters studied. Genotypic coefficient of variation was the major component of total variation however; PCV was invariably higher than GCV for the characters studied. Number of seeds per ear showed high PCV followed by ear weight, yield and number of productive tillers per plant while days to fifty per cent flowering recorded lowest PCV. Ear weight, thousand grain weight, protein content, days to maturity, days to fifty per cent flowering and yield showed high heritability and genetic advance. While, number of productive tillers per plant, leaf number per tiller, Length of flag leaf and finger length showed moderate heritability coupled with moderate genetic advance. Plant height, culm thickness, width of flag leaf, peduncle length, finger width and number of seeds per ear showed low heritability coupled with low genetic advance. Number of productive tillers per plant, leaf number per tiller, thousand grain weight and ear weight showed highly significant positive correlation with yield. Grain yield was found positively associated with days to fifty per cent flowering, plant height, culm thickness, peduncle length, finger number per ear, finger width and protein content. While days to fifty per cent flowering recorded highly significant positive correlation with days to maturity. Days to fifty per cent flowering, flag leaf width, number of productive tillers per plant, number of leaves, peduncle length and culm thickness showed positive direct effect on yield. Number of productive tillers per plant, leaf number per tiller, days to fifty per cent flowering, days to maturity, finger number per ear, thousand grain weight and ear weight showed positive indirect effect on yield through number of seeds per ear. In protein analysis it was found that the genotype GP-2011-79 found to contain high protein content than the checks. Similarly the genotype GP-2011-19 exhibited highest yield over the checks however the following genotypes GP-2011-294, GP-2011-366 and GP-2011-454 were also found high yielding which can be used as parental stocks in breeding programme. Therefore it can be concluded that selection for reduced plant height, more number of productive tillers per plant, increased ear weight, and more maturity duration would be in favour of achieving higher yield in finger millet.
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    ThesisItemOpen Access
    Studies on protein profiling (SDS-PAGE) combining ability and heterosis in Indian mustard (Brassica juncea L. Czern & Coss)
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2013-06) Meena, Jitendra Kumar; Pant, Usha
    The present investigation was carried out with the aim to evaluate the genetic purity of cross combinations using SDS-PAGE analysis and study of selection parameters, combining ability, extent of heterosis for various quantitative characters in Brassica juncea (L.) Czern & Coss. The experimental material for present study comprised of a set of 15 genotypes of Indian mustard (Brassica juncea L.) of diverse origin involving four early maturing genotypes (NDRE-4, PRE-2007-6, PR-2006-1 and PRE-2009-9), six agronomically superior genotypes (NDYR-8, Kranti, Maya, Bhaghirathi, RGN-74 and RGN-145), two genotypes suitable for late sowing (Vardan and Ashirwad) and three bold seeded genotypes (PRB-2006-5, PRB-2008-5 and PRB-2004-3-4). Crosses were made between these genotypes in all possible combinations excluding reciprocals during rabi 2011-12. The parents and their 105 F1’s along with one standard variety (Kranti) were evaluated in a randomized block design with three replications during rabi 2012-13 at Norman E. Borlaug Crop Research Centre, G.B. Pant University of Agriculture and Technology, Pantnagar. The combining ability analysis was performed by following Griffing’s (1956) method 2 and model 1. Results of ANOVA showed that the mean squares due to gca and sca were highly significant for all the characters except secondary branches per plant, the magnitude of former being higher than later, indicating predominance of additive gene action for the expression of the characters under study. The estimates of 2 sca were found to be higher than the 2 gca for all characters except number of seeds per siliqua and oil content. Predictability ratio was highest for days to 50 percent flowering, number of seeds per siliqua and oil content. It indicated the predominance of fixable variance which can be utilized by following any breeding methodology for improvement of such traits. For rest of the traits the value of predictability was lower than one. It represented the involvement of non-fixible variance so in such characters the heterosis breeding appears to be the best option. Average degree of dominance showed partial dominance(<1.0) for days to 50% flowering, number of seeds per siliqua and oil content and over dominance(>1.0) for primary branches per plant, glucosinolate content, chlorophyll content, siliqua length, length of main raceme, test weight, siliquae on main raceme, secondary branches per plant, seed yield per plant, plant height, days to maturity and siliqua density. Based on significant GCA effects in desirable direction, the parents NDRE-4 (-3.651**) for early maturity; NDRE-4 (-11.077**) for dwarf plant height; Maya (1.796**) for seed yield per plant; NDYR-8 (0.736**), for oil content and Kranti (-7.104**) for low glucosinolate content could be screened as superior donors. The study revealed that crosses Bhaghirathi × PRB-2004-3-4 (-6.252**) for early maturity; PRB-2008-5 × PRB- 2004-3-4 (-30.236**) for dwarf plant height; Vardan × Ashirwad (0.849*) for oil content and Bhaghirathi × RGN-145 (-36.632**) for low glucosinolat content had high magnitude of sca effects. The evaluation of heterosis revealed that heterotic combinations PRB-2004-3-4×PRE-2009-9 (-9.627**), Bhaghirathi × PRE-2009-9 (-16.53**), RGN-145 × PR-2006-1 (-4.64**) for early maturity; PRB-2008-5 ×PRB-2004-3-4 (-24.37**), PRB-2008-5 × PRE-2009-9 (-24.89**), PRE-2009-9 × NDYR-8 (-23.32**) for dwarf plant height; PR-2006-1 × NDRE-4 (201.74**) , PR-2006-1 × NDYR-8 (137.26**), Maya × PRE-2004-3-4 (40.26**) for seed yield; PRE-2009-9 × NDYR-8 (15.84**), RGN-74 × PRE-2007-6 (2.91**), Maya × NDYR-8 (2.09**) for oil content had highest potential for of mid parent heterosis better parent and economic heterosis respectively. SDS-PAGE Electrophoresis was employed to assess the genetic purity of parents and their 105 F1’s. Based on protein profiling, 105 F1’s and 15 parents were characterized by soluble leaf proteins separated on SDS-PAGE. A wide variation was observed in the pattern of protein bands of studied hybrids and parental lines. The hybrids differed in the number of bands, their relative mobility and intensity. In case of hybrids the bands at different rf values distinguished the F1’s from their parental lines. The bands in F1’s at different rf value may be inherited from one or both parents or may be new bands present with different intensity.
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    ThesisItemOpen Access
    Genetic evaluation of advanced breeding lines of mungbean for nitrogen fixation, yield and other quantitative traits
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2013-06) Sahu, Hemant; Panwar, R.K
    The present investigation was carried out at N. E. Borlaug Crop Research Centre of G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India, during kharif, 2011and Kharif 2012 with 35 genotypes (including 32 advanced breeding lines and 3 check varieties) of mungbean for nineteen quantitative characters with the objective to study genetic divergence, variability, character association for yield and its component and their direct and indirect effects on seed yield. The mean squares due to genotypes were highly significant for all the characters studied. In general, phenotypic coefficient of variation was higher than genotypic coefficient of variation in similar direction. Nodule volume exhibited maximum PCV in both the season. Plant height, nodule number and yield/plot exhibited high heritability in combination with high genetic advance in both the season. A comparison of mean performance of genotypes revealed the superiority of genotypes over the checks for various characters in both the season. PM 6 and PM 11-1 were promising genotypes for yield/plot, whereas PM 11-8 and PM 11-20 were promising for yield/plant in both the season. PM 11-8 and PM 11-25 were promising genotype for nitrogen fixation for 2011 and 2012, respectively. The genotypic correlation coefficients were higher than phenotypic correlation coefficients in both the season for most of the characters. Correlation studies showed that grain yield had significant positive association with number of primary branches per plant, number of pods per plant, harvest index and yield per plot. Straw protein showed positive and significant correlation with nitrogen fixation/plant. In the present study it was found that root length, plant height, seed/pod and 100 seed weight are important traits which have direct and positive effect on yield in both the season. Based on D2 statistic, the 35 genotypes were grouped into 14 and 12 clusters in 2011 and 2012, respectively. The maximum numbers of genotypes (19) were grouped in cluster I in 2011 and cluster II in 2012. The maximum divergence was observed between cluster VII and VIII in 2011, while in cluster I and VIII in 2012. During 2011 nodule volume contributed maximum towards genetic divergence followed by nodule dry weight and nitrogen fixation whereas in 2012 nitrogen fixation showed highest contribution followed by nodule dry weight Lowest contribution was made by days to maturity followed by days to 50 % flowering and seed protein in 2011, while seed/pod showed minimum contribution followed by seed protein in 2012. Based on variability, correlation and path coefficient analysis, it was observed that number of pods per plant, number of primary branches, harvest index, seed yield per plant determining yield and were found to be important yield attributing characters for selection in mungbean improvement. We can improve yield by selection and hybridization followed by selection.
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    ThesisItemOpen Access
    Studies on genetic diversity for quantitative and qualitative traits and molecular polymorphism among germplasm lines of yellow sarson (Brassica rapa var.yellow sarson)
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2013-06) Harsha; Pant, Usha
    Present investigation entitled “Studies on Genetic Diversity for Quantitative and Qualitative Traits and Molecular Polymorphism among Germplasm Lines of Yellow Sarson (Brassica rapa var. yellow sarson” was under taken with the objectives to estimate extent of genetic variability, character association with quantitative and qualitative traits and to assess the diversity among the genotypes at molecular level using RAPD markers. The experimental materials consisted of 31 yellow sarson germplasm line including 2 check varieties namely PPS-1 and B-9 under two environmental condition i.e. timely sown (E1) and late sown (E2) condition. All the genotypes were evaluated in a randomised block design during rabi season 2012-2013 at Norman E. Borlaug Crop Research Centre, G. B. Pant University of Agriculture and Technology, Pantnagar. The quantitative, quantitative and molecular data were analyzed using appropriate statistical techniques. The results revealed presence of significant variations for all the characters except primary branches per plant and oil content. High values of phenotypic coefficients of variability (PCV) closely followed by genotypic coefficient of variability (GCV) for number of secondary branches per plant, yield per plant, glucosinolate content under timely sown condition as well as late sown condition. The heritability was high for glucosinolate content and days to maturity under in E1 as well as in E2. The glucosinolate content and seed yield per plant showed high genetic advance as percentage of mean in both the condition. The divergence studies of D2 statistics grouped the 31germplam line into 8 and 12 clusters in E1 and E2 respectively. Members of cluster V and VIII (26.812) were found to be most diverse on the basis of their inter cluster distance as opposite to clusters V and I (2.801) which had lowest distance value in E1 whereas in E2 clusters IV and II (51.930) exhibited maximum inter cluster distance as opposite to cluster VI and IX (4.965). The correlation study revealed that seed yield per plant was significantly and positively associated with days to 50% flowering, plant height, main raceme length, siliquae on main raceme, number of primary branches per plant, siliqua length, seeds per siliqua and test weight under E1 as well as in E2. The path coefficient analysis with seed yield as end product revealed that the high positive direct effect was shown by siliqua on main raceme, plant height, seeds per siliqua, number of secondary branches per plant, number of primary branches under timely sown condition and in late sown condition test weight followed by main raceme length, number of secondary branches per plant, days to maturity, number of primary branches per plant, oil content, siliqua length, seeds per siliqua showed positive direct effect on seed yield. Plant height, main raceme length and siliquae on main raceme, showed high positive indirect effects on seed yield. Shannon-Weaver diversity index (H􀀀) was found significantly for all 11 characters. Random Amplified Polymorphic DNA (RAPD) markers were used to assess the molecular diversity amongst 31 germplasm lines. The polymorphism was found to be 100%. The distance coefficient varied from 0.1007 to 0.4430 and ordered the populations of 31 genotypes into three major clusters with varying number of genotypes indicating that RAPD marker as an effective tool for divergence studies.
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    ThesisItemOpen Access
    Genetic divergence analysis of Shisham (Dalbergia sissoo Roxb.) elite clones under agroforestry system
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2012-08) Rai, Tarun Kumar; Tewari, Salil K.
    The present investigation was conducted at Agroforestry Research Centre, Haldi, GBPUA&T, Pantnagar, Distt. Udham Singh Nagar, Uttarakhand. The field experiment was conducted during month of July 2011 to June 2012. The experimental materials consisted of 41 diverse genotypes collected from different parts of India. One year old plants were planted in Randomized Block Design with four replications in 2008. Four trees in block were maintained at 4x4 spacing with objective to evaluate the performance of 41 elite clones of Shisham under agroforestry system in reference to genetic variability, heritability, genetic advance, character association and their partitioning into direct and indirect effects of component characters on tree volume over bark, genetic divergence, biomass and carbon stock and wheat component traits under Shisham based agroforestry system for yield Wheat crop was sown on 10th Nov’2011 as an intercrop. It was found that significant genotypic differences, supported by wide range of variation of mean values for all the characters indicated substantial inherent differences for characters under study in the experimental material. High estimates of heritability coupled with high genetic advance were observed in girth at ground level, volume over bark, diameter at ground level, bole surface area, branch angle, diameter at breast height, girth at breast height, basal area, crown length, volume under bark and tree height. Growth character – tree height, diameter at breast height and girth at breast height were found significantly and positively correlated with tree volume over bark, suggested the importance of these traits in formulating the selection indices for volume. Path – analysis revealed that in all set of data, girth at ground level, diameter at breast height, tree height, clear bole length, basal area, bole surface area, branch angle and volume under bark had direct positive effects on tree volume over bark. Based on Mahalanobis D2 analysis different clusters were formed and clustering pattern indicated substantial genetic diversity in the experimental material. The 41 genotype were grouped in 7 clusters. Intra-cluster distance ranged from 0 to 11.91. It was maximum in cluster III (11.91). Though per cent contribution calculated by modified method revealed that in addition to girth at ground level, other characters like diameter at ground level, girth at breast height, diameter at breast height, tree height, clear bole length, crown length, number of primary branches, bole straightness and basal area contributed substantially towards genetic divergence. Genotypes PS-54, PS-50, 19, PS-90, PS-48, 11, PS-2, 1009, PS-6, 5, 57, PS- 38, PS-46, 1010, PS-76 and 12 were highly divergent and better performing. Among the different aboveground components bole biomass contributed maximum 48.6-55.7 per cent towards total above ground biomass followed by branch contributing 31.1- 34.9 per cent, leaf contributing 3.02-7.23 per cent and twig contributing 2.1-6.2 per cent. Among the different belowground components the stump root biomass contributed (70.47-90.03 per cent) maximum towards total belowground biomass followed by lateral contributing 11.56- 25.35 per cent, fine root contributing 1.06-4.18 per cent. The above ground and below ground biomass in Shisham was found to be maximum in genotype PS-90. The data of aboveground carbon sequestration were showed that the contribution were found maximum in bole>branch>leaf>twig. Among the different belowground components stump root carbon contributed (70.88- 91.22 per cent) maximum towards total aboveground carbon sequestration followed by lateral root (8.69- 25.09 per cent) and fine root (1.01-4.01 per cent). The above ground and below ground carbon sequestration in Shisham was found to be maximum in genotype PS-90. The more the biomass production, the more will be the carbon sequestration. The wheat yield was significantly reduced by shading.