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ThesisItem Open Access ECONOMIC OF BT (BACILLUS THURINGIENSIS) COTTON CULTIVATION IN MAJOR COTTON GROWING DISTRICTS OF PUNJAB(PUNJAB AGRICULTURAL UNIVERSITY, LUDHIANA, 2010) PRABHJOT SINGH; GROVER, D. K.ThesisItem Open Access COMPARATIVE STUDY OF NUTRITIVE VALUE OF TRADITIONAL AND MODERN SNACKS(PUNJAB AGRICULTURAL UNIVERSITY; LUDHIANA, 2010) SISODIA, POONAM; BRAR, JASWINDER KAUR06062017_0002_0024_Abstract.pdfThesisItem Restricted CANOPY MANAGEMENT STUDIES IN PATHARNAKH PEAR(PUNJAB AGRICULTURAL UNIVERSITY; LUDHIANA, 2010) SHEHBAZ SINGH; GILL, P.P.SThesisItem Open Access Heterosis and Combining ability studies in Garden Pea (Pisum sativum L.)(PAU, 2010) DineshEighteen genotypes of pea were selected and crossed in line x tester fashion using fifteen females and three males during November 2007. Forty-five hybrids along with eighteen parents were grown at the Vegetable Research Farm, Punjab Agricultural University, Ludhiana during Nov, 2008. Significant mean squares due to lines, testers, hybrids, line x tester and parents vs. hybrids were observed for all the characters. The ratio of 2 sca/2 gca was more than unity in number of seeds per pod, nod at which first pod developed and for 100 seed weight, indicating non-additive gene action, while additive gene action was important for pod length, number of pods per plant, green pod yield, shelling percentage and crude protein. Among the females, ARKEL was found to be the best general combiner for days taken to 50% flowering, node at which first flower appears, and days taken to maturity. JP-501A/2 was found to be good combiner for pod length and number of pods per plant. Among the males, C-96 was good combiner for days taken to 50 per cent flowering, plant height, node at which 1st flower appears, pod length, shelling percentage, 100 seed weight and alcohol insoluble matter. The line C-400 found to be the best combiner for number of pods per plant, green pod yield and crude protein. The cross P1 x C-400 exhibited highest and significant sca effect for days taken to 50 % flowering and crude protein, KS-268 x PB- 89 for plant height and alcohol insoluble matter. ARKEL x PB- 89 for node at which first flower appears, C-308 x PB- 89 for pod length and ARKEL x C- 400 for number of pods per plant, P2 x PB- 89 for shelling percentage. The best heterotic combination for days taken to 50 % flowering was P1 x C- 400, days taken to maturity was ARKEL x C- 96, C- 308 x C- 400 for plant height, JM- 5 x C-96 for shelling percentage, MA- 6 x PB- 89 for node at which first flower appears, MA-6 x C- 96 for number of pods per plant, MA- 6 x C- 400 for pod length. The best heterotic combination for days taken to 50 % flowering was P1 x C- 400, days taken to maturity was ARKEL x C- 96, C- 308 x C- 400 for plant height, JM- 5 x C-96 for shelling percentage, MA- 6 x PB- 89 for node at which first flower appears, MA-6 x C- 96 for number of pods per plant, MA- 6 x C- 400 for pod length, PB- 88 x PB- 89 for number of grains per pod and KS- 268 x C- 400 for green pod yield, AP1 x C- 96 for crude protein and PMR- 19 x PB- 89 for alcohol insoluble matter. These can be utilized for further breeding programmesThesisItem Open Access DEVELOPMENT OF HYBRIDS OF PLEUROTUS FLORIDA AND VOLVARIELLA VOLVACEA THROUGH PROTOPLAST FUSION(2013) Zenebe Teka MengeshaProtoplast fusion using Polyethylene glycol (PEG) and electrofusion was carried out between strains of Pleurotus florida and Volvariella volvacea to develop improved hybrids. Preliminary screening was carried out based on responses to growth at different temperature regimes, colony radial growth, morphology and growth patterns of colony, hyphal size, presence or absence of clamp connections, and other unique characters like production of reddish exudates. Following the rigorous screening, eight intergeneric hybrids (Pv14, Pv19, Pv21, Pv27, Pv45, Pv66, Pv102 and Pv127) were selected and further evaluated for fruit body formation. Pv45, Pv66 and Pv102 were unable to give primordia after 25 days of growth and were considered sterile. Among those which developed fruit bodies, Pv21 and Pv127 had brownish circular caps with regular margins and central stipes. Other strains showed cap morphologies closely related to PF5. Isozyme (alcohol dehydrogenase, malate dehydrogenase, esterase and super oxide dismutase) pattern analyses indicated presence of bands in all the strains which verified hybridization between the two parents. Besides, two bands observed neither in the parents nor in the other strains were noticed in pv27 and pv127. Amplifications of genomic DNA from all the strains using 14 arbitrary decamer RAPD primers resulted in 98 bands, with size range of 160bps to 2500bps, of which 57.14 per cent were polymorphic. The bands were obtained only from 8 primers. A combined clustering analysis of these bands using NTSYSpc software indicated that Pv127 exhibited marked divergence from the other strains in the phylogenetic clusters and had better performance. Therefore, the salient investigation of the current work was screening of a hybrid strain (Pv127) with high BE (52.71%) and low temperature tolerance compared to V. volvaceaThesisItem Restricted DEVELOPMENT OF INSTANT KHEER MIX USING EXTRUSION TECHNOLOGY(2013) simta singlaExtrusion technology was used to optimize the process for preparation of precooked rice granules for making instant kheer mix. Kheer is a traditional Indian dessert made from milk, sugar and rice. It generally takes long time for cooking. With this study, process to render this product instant was optimized. Effect of moisture (16-20%), screw speed (100-300rpm) and temperature (130-170⁰C) on bulk density (BD), Water Absorption Index (WAI), Water Solubility Index (WSI) and starch conversion (SC) was studied. BD, WAI and SC increased with increasing moisture. Increase in screw speed and temperature resulted in decrease in BD and increase in WAI, WSI and SC. Interactions of any two independent variables were also studied for any significant effect on any of the dependent variables. Interaction of feed moisture and screw speed was found to significantly affect BD and WAI in a positive manner. Process was optimized for minimum BD and maximum WSI and SC. The most suitable working conditions were found to be 17.7% moisture, 285-300 rpm screw speed and 170⁰C temperature. Further ratio of extrudate, Skimmed Milk powder (SMP) and sugar was standardized. The final mix contained extrudate, SMP and sugar in ratio 45:35:20, reconstitution was done in standardized milk at 80⁰C, with mix to milk ratio of 1:3. Storage studies were conducted for a period of three months. During the storage period, the mix was analyzed for its moisture content and overall acceptability at monthly intervals. Moisture showed slight increase by end of third month. Overall acceptability on the basis of sensory evaluation decreased slightly during storage but remained within acceptable range.ThesisItem Restricted OPTIMIZATION OF CONVECTIVE-CUM-MICROWAVE DEHYDRATION OF OYSTER MUSHROOMS (Pleurotus sajor-caju)(2013) Sakti BansalInvestigations were carried out to study the effect of drying parameters viz. loading density, air velocity, power level on convective-cum-microwave dehydration of oyster mushroom. Multi layer drying of commercially grown mushroom cv. Pleurotus sajor-caju was carried out at 3 levels of loading densities (26-52 kg/m2) and air velocities, 3-5 m/s and microwave drying was carried out at 3 power levels (270-810 W). The drying parameters studied were drying rate, temperature and relative humidity of outgoing air, product temperature and heat utilization factor. Quality attributes included rehydration ratio, shrinkage ratio, texture (hardness and chewiness), colour and overall acceptability. Multi layer drying of mushroom with an initial moisture content of 91% wb was carried out at 60°C in a mechanical drier till the moisture content reached 27% wb followed by drying in microwave oven to a final moisture content of 6.89% wb. It was observed that drying rate, relative humidity of outgoing air and heat utilization factor decreased with drying time but opposite trend was observed for temperature of outgoing air and product. The decline in drying rate increased with increase in air velocities and power level whereas opposite trend was observed for loading densities. Increase in loading density and air velocity resulted in decrease in heat utilization factor and relative humidity however temperature of outgoing air and product showed the opposite trend. Logarithmic model showed the highest adequacy of fit for multi layer drying and two term exponential model for microwave drying. The effective moisture diffusivity increased with increase in both loading density and air velocity. The increase in loading density resulted in increase in rehydration ratio, shrinkage ratio, colour change but decrease in texture and overall acceptability. The increase in air velocity resulted in increase in rehydration ratio, hardness, colour change overall acceptability but decrease in shrinkage ratio and chewiness. The increase in power level resulted in increase in rehydration ratio, texture, colour change and decrease in shrinkage ratio and overall acceptability. The optimum conditions for convective-cum-microwave drying of mushroom were found to be 38.80 kg/m2 loading density, 3.86 m/s air velocity and 413.6 W power level. Keywords: Oyster mushroom, drying kinetics, quality, optimizationThesisItem Open Access MODELLING THE IMPACT OF CLIMATE CHANGE ON GROUNDWATER RESOURCES IN CENTRAL PUNJAB(2013) samanpreet kaurA study was planned to assess the impacts of climate change on groundwater resources in Ludhiana district under PRECIS A1B climate change scenario by linking the outputs of climate, soil-water-vegetation and groundwater models using GIS. Bias correction of climate data was done by correction functions, developed using modified difference, approach at daily time scale for rainfall and at monthly time scale for Tmax and Tmin. The methodology consists of development of simulation zones by overlaying soil, drainage, canal and landuse thematic maps in GIS and estimating water balance components using CropSyst and well defined norms. The groundwater draft for individual simulation zones was computed on the basis of landuse, irrigation requirement and rainfall conditions. The spatial distribution of recharge and groundwater draft was mapped to GIS and was provided as input to groundwater model. The results showed that temperature and rainfall would be increased by 2.4°C, 315 mm in MC (2021-2050); and 5.1°C and 465 mm in EC (2071-2098), respectively. During kharif season increase in RF would be 28.0% in MC and 52.0% in EC compared to that in PTS (1971-2010). The corresponding values during rabi would be 82.3% and 88.6%, respectively. As a consequence of increased precipitation, the irrigation requirements in MC and EC would decrease by 46% and 45% during kharif; and 21% and 34% in rabi, respectively. However, the potential groundwater recharge would be reduced by 23.1 and 4.2 per cent in kharif, and increased by 39.1 and 79.6 per cent in rabi during MC and EC, respectively. The average groundwater levels would decrease by 2.2 m in 2050 and increase by 4.8 m in 2098, compared to base year of June 2000. The results presented here should be interpreted as trends and not as accurate quantitative predictions of the hydrological changes as there are numerous sources of uncertainties associated with climate change prediction. Keywords: Climate change, Groundwater, MODFLOW, CropSyst, Geographical information systemThesisItem Open Access EFFECT OF NITROGEN ON GROWTH, YIELD AND QUALITY OF INDIAN MUSTARD (Brassica juncea L.) GENOTYPES(2013) Parminder KumarThe field experiment entitled “Effect of nitrogen on growth, yield and quality of Indian mustard (Brassica juncea L.) genotypes” was conducted at Punjab Agricultural University Ludhiana (30o 56/ N latitude and 75o 48/ E longitude and at an altitude of 247 metres above mean sea level), during rabi 2011-12 on loamy sand, neutral in reaction, low in available N, rich in available P and K soil. The study was conducted in split plot design in three replications with four doses of nitrogen (0, 50, 100 and 150 kg/ha) in the main plots and five genotypes (RLC 1, RLC 11, RLC 12, PBR 210 and PBR 91) in the sub plots. Application of N significantly increased plant height, dry matter accumulation (DMA), leaf area index (LAI), photosynthetically active radiation (PAR) interception and chlorophyll content up to 100 kg/ha though such an increase, except plant height continued upto 150 kg/ha of N. Nitrogen application delayed initiation of flowering and significantly increased number of the secondary branches and siliquae per plant and, seeds per siliqua over control. Seed yield increased by 65.7 and 12.0 per cent with 100 kg/ha of N over control and 50 kg/ha of N, respectively. There was consistent reduction in seed oil content and significant increase in oil yield with N application of 50 kg/ha over control. Nitrogen, protein and linoleic acid content increased with increase in N levels. Among genotypes, PBR 91 and PBR 210 exhibited significantly better growth in terms of plant height, DMA and LAI with higher PAR as compared to RLC 11 and RLC 12. Genotypes, PBR 91, PBR 210 and RLC 1 took significantly more number of days for completion of flowering than other genotypes. RLC 12 produced significant higher number of primary and secondary branches per plant, RLC 11 produced higher number of seeds per siliqua. Genotypes PBR 91, RLC 1 and PBR 210 registered significantly higher 1000-seed weight than RLC 11 and RLC 12. Differences in seed yield among genotypes were non-significant. Genotype RLC 11 registered significantly higher oil content but lowest seed protein content. Genotype RLC 12 contained significantly higher seed protein content than rest of the genotypes. Genotypes PBR 91, RLC 11 and PBR 210 produced similar but significantly higher oil yields than RLC 1. Erucic acid content was significantly lower in RLC 1 and RLC 12 while glucosinolate content was lowest in RLC 11 followed by RLC 12. Nitrogen application increased the seed yield up to 100 kg/ha in RLC 1 and RLC 12 and up to 150 kg/ha in RLC 11, PBR 210 and PBR 91. Increase in seed yield with 50 kg/ha of applied N over control was significant in all genotypes whereas further increase in N to 100 kg/ha increased the seed yield significantly in case of RLC 11, RLC 12 and PBR 210. Keywords: Indian mustard, nitrogen, genotypes, dose, growth, yield, quality, N uptake
