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Subject: Vegetable Science ×

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    ThesisItemOpen Access
    ASSESSMENT OF GENETIC VARIABILITY AND MOLECULAR CHARACTERIZATION IN PEA (Pisum sativum L.)
    (SARDAR VALLABHBHAI PATEL UNIVERSITY OF AGRICULTURE AND TECHNOLOGY, MEERUT- 250110 (U.P.),, 2022-07) ASSESSMENT OF GENETIC VARIABILITY AND MOLECULAR CHARACTERIZATION IN PEA (Pisum sativum L.); Dr. Bijendra Singh
    ABSTRACT Name: Rupesh Kumar ID. No.: PG/H-5352 Degree: M.Sc. (Hort.) Veg. Sci. Year of admission: 2020-20211 Department: Vegetable Science Major : Vegetable Science Advisor : Dr. Bijendra Singh Thesis Title: "Assessment of genetic variability and molecular characterization in College: Horticulture Minor: Biotechnology Pea (Pisum sativum L.)." The present study by using 20 germplasms of pea were evaluated for genetic diversity and yield related traits with randomized block design (RBD) with three replications during Rabi 2021-22. Ten quantitative characters were considered to study information on the nature and magnitude of genetic variability, degree of association among different yield contributing traits with yield and related traits and the degree of genetic divergence and molecular analysis at molecular level. Observations were recorded on various characters viz. days to germination, days to 50% flowering, plant height (cm), number of pods per plant, seeds per pod, length of pod (cm), width of pod (cm), days to maturity, pod yield per plant (g) and pod yield (q/ha). Analysis of variance revealed substantial amount of variability among the genotypes for all the characters, under study, indicated wide spectrum of variability among the genotypes. High phenotypic and genotypic coefficient of variation was observed for number of pod per plant (26.63, 26.43), moderate PCV and GCV was recorded for days to 50 % flowering (18.32, 18.15) and least PCV and GCV was observed in (6.20, 5.33). High heritability coupled with high genetic advance as percentage was observed for days to maturity (99.39, 27.65), plant height (98.98, 46.50), pod yield per plant (98.95, 45.43), pod yield (q/ha) (98.89, 32.22) and moderate heritability was recorded for number of pod per plant (98.80, 46.50) days to 50 % flowering (98.25, 37.07), length of pod (89.35, 13.99). Days to maturity revealed highly significant and positive correlation with days to 50% flowering (0.736, 0.726) both genotypic and phenotypic level. Path coefficient analysis revealed that length of pod (0.9703) have direct and positive effect on pod yield at genotypic level and number of pods per plant (0.5881) have direct and positive effect on pod yield at phenotypic level. Mahalanobis (D*) statistics revealed considerable genetic diversity among the genotypes. Genotypes were grouped into five clusters maximum 5 genotypes fell in to two cluster viz. cluster I and II. In the present study, maximum intra cluster distance was observed for cluster I and the maximum inter cluster distance was recorded in cluster II and IV and least inter cluster distance observed between cluster III and V. At Molecular level 11 RAPD primers produce 60 bands. Average of PIC value for 11 RAPD primers 0.330. Average resolving power is 2.590 for RAPD primers. Unweighted pair-group method arithmetic average cluster analysis and principal coordinate analysis on the marker-based GS grouped the cultivated varieties separately from whole accessions into different clusters. Genotype AP-3 and Arka Priya revealed great distance genetic relationship. ul (Dr. Bijendra Singh) (Rupesh Kumar) Author Advisor VITAE
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    ThesisItemOpen Access
    ANALYSIS OF GENETIC DIVERSITY IN POTATO
    (SARDAR VALLABHBHAI PATEL UNIVERSITY OF AGRICULTURE AND TECHNOLOGY, MEERUT- 250110 (U.P.),, 2022-09) MR. RAHUL KUMAR; Dr. Satya Prakash
    ABSTRACT Name: Rahul Kumar ID. No. PG/H- 5351/20 Semester & Year of admission: 2020-21 Degree: M.Sc. (Horti.) Vegetable Science Major: Vegetable Science Department: College of horticulture Minor: Genetics and Plant Breeding Advisor: Dr. Satya Prakash Thesis tittle: “Analysis of Genetic Diversity in Potato (Solanum tuberosum L.)”. Twenty one genotypes of potato were evaluated at Horticulture Research Centre of SVP University of Agriculture & Technology, Meerut during Rabi season 2021-22.The experiment was carried out in Randomized Block Design with three replications. Potato (Solanum tuberosum L. 2n=4x=48) is known as the "King of vegetables" and belongs to the solanaceae family. The origin of Potato is central Andean region of South America. The analysis of variance revealed significant among all the genotypes for all the 11 characters under study. The high percentage (>20%) of genotypic coefficient of variation and phenotypic coefficient of variation for length of internodes, plant height, number of stem plant-1, tuber yield plant-1, number of nodes at 30 DAP, number of tubers plant-1 while moderate (10-20%) for number of leaves at 60 DAP, number of nodes at 60 DAP, tuber size, number of leaves at 30 DAP and lowest (<10) in germination % at 20 DAP. Further the present finding exhibited that the estimate of PCV was magnitude greater than the GCV for all characters indicating the important role of environment the expression of characters. So that character would be more effective to improve the crop. The range of heritability varies from 40.23% for germination % at 20 DAP to 98.85% for tuber yield plant-1. The highest heritability estimates for tuber yield plant-1, number of leaves at 60 DAP, number of nodes at 60 DAP, plant height, number of nodes at 30 DAP, tuber size, length of internodes, number of tubers plant-1, number of stem plant-1, number of leaves at 30 DAP. The range of genetic advance was varies from 6.19% for germination % at 20 DAP to 59.39% for length of internodes. The estimates of genetic advance as percent of mean was exhibited high for length of internodes, plant height, tuber yield plant-1, number of stem plant-1, number of nodes at 30 DAP, number of tubers plant-1, number of leaves at 60 DAP, number of nodes at 60 DAP, tuber size. In general, genotypic correlation coefficients were found to be greater than phenotypic correlation coefficients. Tuber yield plant-1 showed positive and highly significant correlation with tuber size, number of leaves at 60 DAP, number of tubers plant-1, germination % at 20 DAP while, positive and less-significant correlation with number of leaves at 30 DAP. Path analysis result was obtained positive and direct effect on tuber yield plant-1 was exerted by the length of internodes, tuber size, number of tubers plant-1, number of nodes at 30 DAP, number of leaves at 60 DAP, germination % at 20 DAP and negative direct effect on tuber yield per plant was recorded by the plant height, number of nodes at 60 DAP, number of stem plant-1 and number of leaves at 30 DAP. Twenty one genotypes of potato were grouped into five clusters. The cluster I comprised highest eight genotypes, cluster V comprised five genotypes, Cluster III comprised four genotypes and minimum genotypes comprised in Cluster II and Cluster IV respectively. The range of intra-cluster distance was from 1.545 to 2.248. The maximum intra-cluster distance was recorded in cluster I, cluster V, cluster II, cluster IV and minimum intra-cluster distance was found in cluster III. The maximum inter-cluster distance was observed in cluster IV and II and the minimum inter-cluster distance was found in cluster III and I. (Satya Prakash) (Rahul Kumar) (Advisor) (Author)
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    ThesisItemOpen Access
    EFFECT OF NUTRIENT MANAGEMENT ON GROWTH, YIELD AND QUALITY OF GARLIC (Allium sativum L.) CV. YAMUNA SAFED
    (SARDAR VALLABHBHAI PATEL UNIVERSITY OF AGRICULTURE AND TECHNOLOGY, MEERUT- 250110 (U.P.),, 2022-07) ASTHA VISHWARAJ; Dr. Manoj Kumar Singh
    Abstract Name: Astha Vishwaraj ID. No.: 5350 Year of admission: 2020-21 Degree: M.Sc. (Horti.) Vegetable Science Major: Vegetable Science Department: Vegetable Science Minor: Soil Science and Agricultural Chemistry Advisor: Dr. Manoj Kumar Singh Thesis Title: “Effect of nutrient management on growth, yield and quality of garlic (Allium sativum L.) cv. Yamuna Safed-3’’ A field experiment was conducted at Horticultural Research Centre, SVP University of Agriculture & Technology, Meerut (UP) during the year 2021-22 for evaluating the effect of nutrient management on growth, yield and quality of garlic (Allium sativum L.) cv. Yamuna Safed- 3”. The total numbers of treatments were nine laid out in different combinations of inorganic and organic fertilizers and was replicated three times in Randomized Block Design (RBD). The application of treatment 75% RDF (75:40:40:25 NPKS kg/ha) + FYM (2.5 t/ha) + PM (0.5 t/ha) (T7) was found significantly superior in terms of plant height, number of leaves, length of leaves and width of leaf, height and width of collar, fresh weight of plant, earliness in maturity, weight of bulbs and size of bulbs, number of cloves, weight of cloves and size of cloves, TSS, ascorbic acid content, A and B grade bulbs of garlic crop as compared to control and other treatments. The highest yield 160.52 q ha-1 and B:C ratio 2.40 was observed with the application of T7-75% RDF (75:40:40:25 NPKS kg/ha) + FYM (2.5 t/ha) + PM (0.5 t/ha). (Manoj Kumar Singh) (Astha Vishwaraj) Advisor Author
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    ThesisItemOpen Access
    MORPHOLOGICAL AND MOLECULAR MARKER BASED SCREENING OF TOMATO GENOTYPES (Solanum lycopersicum L.) FOR Ty-2 GENE
    (SARDAR VALLABHBHAI PATEL UNIVERSITY OF AGRICULTURE AND TECHNOLOGY, MEERUT- 250110 (U.P.),, 2023-09) DEEPAK KUMAR; Dr. Vipin Kumar
    ABSTRACT Thesis Title: Morphological and Molecular Markers based Screening of Tomato Genotypes (Solanum lycopersicum L.) For Ty-2 Gene Tomato (Solanum lycopersicum L.) is a widely cultivated solanaceous vegetable crop worldwide due to its economic value. However, its growth is significantly impacted by the tomato leaf curl virus disease (ToLCV/TYLCV), which is caused by begomoviruses and transmitted by the whitefly (Bemisia tabaci). To address this issue, a study was conducted to identify tomato genotypes with favourable horticultural traits and strong, long-lasting resistance against ToLCV/TYLCV. Twenty-five diverse tomato genotypes, each carrying different variations of the Ty-2 gene, were carefully evaluated through both phenotypic and genotypic screening methods. The aim was to identify genotypes that not only exhibit desirable horticultural characteristics but also possess robust resistance against the ToLCV/TYLCV disease and morphological and molecular marker-based diversity were done. Morphological data pertaining to different horticultural traits and disease symptoms, including the percentage of disease incidence (PDI) and disease severity caused by ToLCV, were documented during the mature stage of tomato plants in a field setting. Simultaneously, the validation of the Ty-2 gene was performed in a molecular laboratory. This process involved verifying the presence and functionality of the Ty-2 gene in the selected tomato genotypes, ensuring its role in conferring resistance against ToLCV. In both seasons, there was sufficient variation for all of the features. Among all characters all had high phenotypic and genotypic coefficients of variation, heritability and genetic advance as percent mean. Therefore, it was concluded that direct selection would be the best method for developing these characteristics. The number of fruits per plant and average fruit weight may be used as the best selection indicators due to their great direct contribution and high correlation with yield/plant. The highest inter cluster distance was recorded between Cluster I and Cluster II and Cluster II and Cluster IV (5.789) revealing that the genotypes included in this cluster are very diverse and were due to both natural and artificial selection forces among the genotypes. The genotypes Arka Rakshak F1, Arka Samrat F1, VRT 70, VRT 52 and VRT 24-1 were found superior for yield and yield contributing attributes for the both rabi season. Among the 25 genotypes 11 genotype, namely VRT 67, VRT 52, VRT 66, Arka Rakshak, VRT 16, VRT 78, VRT 58, Arka Samrat, VRT 17, Punjab Ratta, VRT 62, showed resistant for Ty-2gene, while 3 genotypes, namely, VRT 27, Pant T-3, Punjab Gaurav showed susceptibility for Ty-2gene. Name: Deepak Kumar Id No.: PG/H-5411/20 Batch: 2020-2021 Degree: Ph.D. in Vegetable Science Major: Vegetable Science Department: Vegetable Science Minor: Agricultural Biotechnology Advisor: Dr. Vipin Kumar (Vipin Kumar) (Deepak Kumar) Advisor Author
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    ThesisItemOpen Access
    GENETIC ANALYSIS FOR YIELD AND ITS CONTRIBUTING TRAITS IN PEA (Pisum sativum L.)
    (SARDAR VALLABHBHAI PATEL UNIVERSITY OF AGRICULTURE AND TECHNOLOGY, MEERUT- 250110 (U.P.),, 2023-07) Mr. Khursheed Alam; Dr. Manoj Kumar Singh
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    ThesisItemOpen Access
    EFFECT OF INTEGRATED NUTRIENT MANAGEMENT ON GROWTH, YIELD, QUALITY AND PROFITABILITY OF BULBS OF ONION (Allium cepa L.)
    (SARDAR VALLABHBHAI PATEL UNIVERSITY OF AGRICULTURE AND TECHNOLOGY, MEERUT- 250110 (U.P.),, 2023-08) JAGRAJ SINGH; Dr. Satya Prakash
    A field experiment was conducted at Horticultural Research Centre, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut (U.P.) 250110 during the Rabi seasons of year 2021-22 and 2022-23 to fulfill the various objectives of the study entitled “Effect of Integrated Nutrient Management on Growth, Yield, Quality and Profitability of bulbs of Onion (Allium cepa L.)”. The experiment was laid out in Randomized Complete Block Design (RCBD) and replicated thrice. Total 11 treatments have been tried i.e., T1 - Control, T2 - 100% RDF (NPKS @ 120:60:80:40 kg/ha), T3 - 100% RDF + Biofertilizers (Azospirillum + PSB @ 5 kg/ha each), T4 - 75% RDF + FYM @ 6 t/ha, T5 - 75% RDF + VC @ 2 t/ha, T6 - 75% RDF + Biofertilizers (Azospirillum + PSB @ 5 kg/ha each), T7 - 75% RDF + FYM@ 2 t/ha + VC@ 1 t/ha + Biofertilizers (Azospirillum + PSB @ 5 kg/ha each), T8 - 50% RDF + FYM @ 12 t/ha, T9 - 50% RDF + VC@ 4 t/ha, T10 - 50% RDF + Biofertilizers (Azospirillum + PSB @ 5 kg/ha each) and T11 - 50% RDF + FYM @ 6 t/ha + VC @ 2 t/ha + Biofertilizers (Azospirillum + PSB @ 5 kg/ha each). During the experimentation, various observations on growth, yield, quality, economics of different applied treatments and availability of nutrient in soil at post-harvest stage were recorded during whole of the cropping periods 2021-22 and 2022-23. The treatment T7- 75% RDF + FYM@ 2 t/ha + VC@ 1 t/ha + Biofertilizers (Azospirillum + PSB @ 5 kg/ha each) was found to be most effective in terms of growth behavior, yield attributing characters and quality parameters of onion crop followed by the treatment T6- 75% RDF + Biofertilizers (Azospirillum + PSB @ 5 kg/ha each). (Allium cepa L.) However, the maximum availability of nitrogen, phosphorus, potassium and Sulphur in terms of kilogram per hectare at post- harvest stage and B:C ratio with the highest net return were observed in treatment T7 comprising of 75% RDF + FYM@ 2 t/ha + VC@ 1 t/ha + Biofertilizers (Azospirillum + PSB @ 5 kg/ha each) during both the years of study. On the basis of above findings, it was suggested that the treatment T7- 75% RDF + FYM@ 2 t/ha + VC@ 1 t/ha + Biofertilizers (Azospirillum + PSB @ 5 kg/ha each) may be recommended for obtaining maximum bulb yield and more benefit cost ratio for the commercial cultivation of onion crop in western plain zone of Uttar Pradesh.
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    ThesisItemOpen Access
    STUDIES ON GENETIC DIVERSITY AND MOLECULAR CHARACTERIZATION IN TOMATO (Solanum lycopersicum L.) GENOTYPES
    (SARDAR VALLABHBHAI PATEL UNIVERSITY OF AGRICULTURE AND TECHNOLOGY, MEERUT- 250110 (U.P.),, 2023-09) MOHIT; Dr. Bijendra Singh
    The present investigation was conducted to “studies on genetic diversity and molecular characterization in tomato (Solanum lycopersicum L.) genotypes” 25 genotypes of tomato were used for studies during two successive season 2021-22 and 2022-23. The result indicated that the mean performance was found significant differences for all the traits under study. Thus, it is implying that were ample variability in the genotypes of tomato. The PCV was found higher than GCV for all traits during both years studied. The highest percent of genotypic and phenotypic coefficient of variation was observed for number of leaves per plant, fruit yield (q/ha) whereas, lowest was found in days to 50% flowering. The high heritability coupled with high genetic advance was recorded for number of leaves per plant moreover, moderate genetic advance was recorded for days to 50% flowering. Fruit yield (q/ha) showed positive and highly significant genotypic correlation with average yield per plant and positive non-significant correlation with number of primary, while negative non-significant with plant height. The highest phenotypic positive direct effect on fruit yield per plant was observed in average fruit weight and negative direct effect was exerted by fruit index. According to D2 analysis, there were five clusters formed. The highest mean value for days to 50% flowering, number of branches per plant, fruit length, diameter of fruit, average fruit weight, fruit index, average yield per plant and fruit yield (q/ha) was exhibited in cluster V, for number of leaves per plant, number of fruits per plant observed in cluster I and cluster II for plant height and plant spread. The maximum intra cluster distance was observed in cluster V and minimum was found in cluster III. The maximum inter cluster distance was recorded for cluster V with cluster IV and minimum cluster distance in III with Cluster II. Cluster II comprised of highest 9 genotypes moreover cluster I comprised lowest 3 genotypes. The highest contribution in the manifestation of genetic divergence was exhibited by days to 50% flowering and the minimum contribution by fruit index. At molecular level 14 SSR primers produced 17 alleles. Average PIC values, Heterozygosity, discriminating power for SSR was noted 0.087, 0.089 and 0.021 respectively. Unweighted pair-group method arithmetic average cluster analysis and principal coordinate analysis on the marker- based GS grouped the cultivated varieties separately from whole accessions into different clusters. Gentotypes H-88-78-5, Kashi Chayan and VRT-34 showed great diversity from the other genotypes.