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Open Access
Genetic architecture of quality and yield components and molecular diversity assessment in blackgram (Vigna mungo L. Hepper)
(Palampur, 2022-11-07) SOHARU, ALKA; Sood, V.K.
The present investigation entitled "Genetic architecture of quality and yield components and molecular diversity assessment in blackgram (Vigna mungo L. Hepper)" was undertaken with the objectives to study diversity and quality profile of various land races prevalent in different regions of Himachal Pradesh and to study the nature and magnitude of gene effects for various quality and yield related traits and identification of desirable segregants. The materials comparising of 21 mash landraces along with three checks i.e. Him Mash-1, Palampur-93 and HPBU-111, were raised in a Randomized Block Design with two replications in two environmental (Berthin and Palampur) conditions and six inter-varietal crosses were raised in Compact Family Block Design at CSKHPKV Palampur. Analysis of variance revealed the presence of considerable amount of genetic variability for all the traits. On the basis of mean performance for different traits studied, genotypes viz., Sirmour-1, Kangra-1, Sirmour-2, Chamba-6 and Him Mash-1 were observed to have superior performance for traits like, number of branches per plant, seeds per pod, pods per plant, biological yield per plant and yield per plant. High estimates of PCV, GCV, heritability and genetic advance as % of mean observed for biological yield per plant and harvest index over the environments indicated the presence of additive gene action and sufficient variability for improvement through selection. Correlation and path coefficient studies revealed high positive direct effects of biological yield per plant, branches per plant and pods per plant on seed yield per plant at both the experimental sites. Hence, can be regarded as reliable selection factors for developing high-yielding cultivars. On the basis of DUS parameters, D 2 , PCA and molecular genetic diversity analysis, Sirmour-1, Sirmour-2, Chamba-2, Mandi-1, Kullu-3, Chamba-5, Kangra-3, Chamba-7, Bilaspur-1 and Chamba-8 were found to be diverse genotypes, which can be further utilized in urdbean breeding programme(s). For proximate composition genotypes Chamba-6, Kullu-4, Kinnaur-1, Chamba-3 and Kangra-2 were found superior as they had maximum amount of protein, fat, fiber, ash and carbohydrates, respectively. Among all the genotypes evaluated for cooking quality parameters, six genotypes namely Sirmour-2 for highest L:B ratio, Chamba-2 for high water uptake ratio, Chamba-7 for minimum amount of gruel solid loss, Chamba-8 and Chamba-1 for least cohesiveness and Him Mash-1 for least gumminess and chewiness after cooking were found promising. On the basis of yield and quality parameters genotypes Chamba-6, Sirmour-2 and Him Mash-1 were divulged as superior genotypes which can be firther validated by multilocation environment testing over the years. A simple scaling test employing generation mean analysis revealed that the digenic model (additive-dominance model) was inadequate for all of the traits, confirming the existence of non-allelic interactions for all of the crosses. Only duplicate type of epistasis was reported for three traits in Him Mash-1× Sirmour-2 and Palampur-93 × Kullu-4, four in Him Mash-1 × Kullu-4 and Palampur-93 × Chamba-8, two traits in Him Mash-1× Chamba-8 and Palampur-93 × Sirmour-2. Hence, bi-parental mating followed by selection in advanced generations is recommended.
Open Access
Molecular marker- assisted gene pyramiding of yellow rust resistance genes Yr5 and Yr10 into agronomically superior and potential cultivar HS 240 and a doubled haploid genotype DH 40
(palampur, 2022-08-17) Singh, Kritika; Chaudhary, H.K.
The research endeavour entitled “Molecular marker- assisted gene pyramiding of yellow rust resistance genes Yr5 and Yr10 into agronomically superior and potential cultivar HS 240 and a doubled haploid genotype DH 40” was executed in the Department of Genetics and Plant Breeding, CSK HPKV, Palampur during years 2018 to 2022 with the goal to incorporate genes conferring resistance to yellow rust in wheat into single genetic background and develop homozygous lines following doubled haploidy breeding technology. Marker- assisted backcrossing was employed to introgress Yr5 and Yr10 genes into genetic background of HS 240 and DH 40. Donor genotypes used for transfer of resistance genes were near isogenic lines developed using Avocet variety i.e., Avocet-Yr5 and Avocet-Yr10. Molecular markers STS7/8 and Xpsp 3000 were used for selection of Yr5 and Yr10 genes, respectively. Recovery of recurrent parent genome was quantified using polymorphic microsatellites. 448 SSR markers were used for screening of parental genotype, out of which 69 were polymorphic for HS 240 & Avocet-Yr5 and HS 240 & Avocet-Yr10 whereas, 68 markers for DH 40 & Avocet-Yr5 and DH 40 & Avocet-Yr10. For wide hybridization, intercrossed F1s were crossed with Imperata cylindrica to produce haploids which were further treated with colchicine for the development of homozygous doubled haploid plants. In BC1F1 and BC2F1 generation, plants having target genes were subjected to background selection. Background analysis revealed that the extent of recurrent parent genome recovery ranged from 63.07- 84.06 percent, 62.32- 83.33 percent, 60.29- 83.03 percent and 63.97- 83.82 percent for crosses HS 240*2/Avocet Yr5, HS 240*2/Avocet-Yr10, DH 40*2/Avocet-Yr5 and DH 40*2/Avocet-Yr10, respectively in BC1F1 generation. In second backcross, recovery percentage of recurrent parent genome ranged from 84.06- 92.03 percent, 84.78-92.03 percent, 84.56-91.91 percent and 86.76-93.38 percent in crosses HS 240*3/Avocet Yr5, HS 240*3/Avocet-Yr10, DH 40*3/Avocet-Yr5 and DH 40*3/Avocet-Yr10, respectively. Selected individuals having common recurrent parent were intercrossed to produce pyramided lines. The pyramided F1s were further subjected to development of doubled haploids. Various haploidy and doubled haploidy parameters like pseudoseed formation frequency (PFF), embryo formation frequency (EFF), haploid regeneration frequency (HRF), haploid formation frequency (HFF), percent survived plants (PSP) and doubled haploids formation frequency (DHFF) observed in cross (HS 240*3/Avocet-Yr5)//(HS 240*3/Avocet-Yr10) were 55.07 percent, 44.99 percent, 54.54 percent, 21.82 percent, 37.50 percent and 38.89 percent, respectively. While in cross (DH 40*3/Avocet-Yr5)//(DH40*3/Avocet-Yr10), 48.38 percent, 51.17 percent, 51.63 percent, 22.88 percent, 34.29 percent and 41.67 percent of PFF, EFF, HRF, HFF, PSP and DHFF were recorded on wide hybridization with I. cylindrica. Seven doubled haploid plants were developed from F1s of (HS 240*3/Avocet-Yr5)//(HS 240*3/Avocet-Yr10) and five for (DH 40*3/Avocet Yr5)// (DH40*3/Avocet-Yr10). The results of present investigation suggested that marker- assisted backcrossing and doubled haploidy breeding technology hasten the recovery of recurrent parent genome along with screening of target genes at early stage; and reducing number of generation & time for gene introgression in homozygous state thereby accelerating rapid line development.
Open Access
AMMI biplot analysis and phenotypic stability for seed yield and its component traits in linseed (Linum usitatissimum L.) under conventional and natural production systems
(palampur, 2022-04-29) Thakur, Garima; Paul, Satish
The present investigation entitled, “AMMI biplot analysis and phenotypic stability for seed yield and its component traits in linseed (Linum usitatissimum L.) under conventional and natural production systems” was undertaken during rabi 2019-20 and rabi 2020-21 to study the GE interactions for thirty linseed genotypes comprising of advanced breeding lines, elite cultivars and exotic lines across four different locations in Himachal Pradesh with varying altitudes viz., Palampur Bajaura, Kangra and Dhaulakuan with two production systems viz., conventional and Zero Budget Natural Farming (ZBNF) at each location. Therefore, the genotypes were evaluated over a total of sixteen environments (sites-years-production systems) for different yield and component traits. Stability analysis was carried out using Eberhart and Russell and AMMI model. Pooled analysis of variance as per both the models revealed significant differences among the genotypes, environments and GE interaction.As per Eberhart and Russell (1966) model the genotype identified as stable for seed yield was Surbhi. None of the genotypes were found stable for primary branches per plant, secondary branches per plant, capsules per plant, 1000 seed weight and oil content. Whereas, as per AMMI model stable genotypes were identified for all the traits. The most stable genotypes identified with respect to seed yield per plant were Giza-7, KL-278, Him Alsi-2, Janki and KL-279 and the most promising genotypes for oil content were Nagarkot, KL-257, KL-269, Him Alsi-1 and KL-279. Genotypes identified stable as per both the models were Him Alsi-2 for plant height, technical plant height and seeds per capsule, KL-285 and Nagarkot for plant height and Himani for harvest index. Genotypes showing specific adaptations were also identified. For site Palampur genotype Giza-8, for location Bajaura Him Palam Alsi-1(KL-241) and KL-311, for Kangra location KL-317 and Surbhi; for site Dhaulakuan genotypes KL-236, KL-315 and KL-284 showed specific adaptation under conventional system. However, under natural farming system for site Palampur genotypes Baner and KL-285, for site Bajaura genotype KL-284; genotypes KL-257 and KL-285 for location Kangra and Bhagsu, Nagrakot, Him Palam Alsi-2 (KL-263) and KL-284 for site Dhaulakuan showed specific adaptation. However, cross-overs were observed across years. Therefore, further evaluation for more years is recommended. Genotype KL-284 showed consistensy in its rank at location Bajaura under both production systems and hence, could be recommended for conventional as well as natural production systems with respect to site Bajaura. In terms of the environments, natural production system was observed as unfavourable environment for seed yield due to its consistent poor performance across years in comparison to the conventional system. It was also identified as poor discriminating environment. Genotypes KL-314 and Him Palam Alsi-2 (KL-263) were found to be resistant in all four locations for both powdery mildew and rust under epiphytotic conditions
Open Access
Molecular diversity analysis and biochemical profiling of Sri Lankan and Indian tea (Camellia sinensis (L.) O. Kuntze) genotypesMolecular diversity analysis and biochemical profiling of Sri Lankan and Indian tea (Camellia sinensis (L.) O. Kuntze) genotypes
(palampur, 2022-06-27) Dananjaya, Kottawa Arachchige Jeevan; Chaudhary, Harinder Kumar
The present investigation entitled, “Molecular diversity analysis and biochemical profiling of Sri Lankan and Indian tea (Camellia sinensis (L.) O. Kuntze) genotypes” was undertaken with the objectives of molecular and biochemical characterization of Sri Lankan and Indian tea germplasm and identification of potential genotypes to expedite its genetic improvement. Total of 171 tea genotypes comprising 94 Sri Lankan and 77 Indian genotypes were used to assess molecular diversity. Based on 16 morphological characters, first three principal components explained 57.61 % and 58.43 % of the total variability of Sri Lankan and Indian genotypes, respectively. Young shoot colour recorded the highest Shannon’s index (H’=1.14) and (H’=1.16) among morphological descriptors of Sri Lankan and Indian genotypes, respectively. Based on biochemical characterization, genotypes viz. PBGT35, PBGT48, PBGT53 and PBGT71 recorded low caffeine content. Total of 28 polymorphic SSRs i.e.10 unigenic SSRs and 18 functionally relevant SSR amplified 94 different alleles and were developed for molecular diversity and population structure analysis. Based on the SSR data of 171 accessions, all Sri Lankan genotypes were grouped in a single cluster whereas Indian genotypes were grouped in two distinct clusters through structure analysis and principle coordinate analysis (PCoA). Five core collections were developed by different methods. Based on the evaluation parameters, the core collection derived using Gower’s method was the most representative method of the initial collections of Sri Lanka and India. Among selected 21 Sri Lankan core genotypes, 11 accessions belong to introductions followed by 5 TRI developed cultivars and 5 estate selections. Among selected 18 Indian core genotypes, 11 accessions are of China types followed by 2 Assam types and 5 recommended cultivars.
Open Access
Combining ability and gene action studies for economic traits and disease resistance in linseed (Linum usitatissimum L.)
(palampur, 2019-05-02) Sran, Ranjeet Singh; Satish Paul
The present investigation entitled “Combining ability and gene action studies for economic traits and disease resistance in linseed (Linum usitatissimum L.)” was undertaken to get the information on nature and magnitude of gene action, combining ability and heterosis and thereby identifying potential parents and cross combinations for seed yield, fibre yield and associated traits under variable environments that could support further improvements of linseed productivity using appropriate breeding methodology. Eight linseed varieties viz., Surbhi, Bhagsu, Nagarkot, T-397, Himani, Baner, JRF-4 and JRF-1 were hybridized following Griffing’s Method 2, Model 1 in a half diallel fashion. Experimental materials comprising of eight linseed varieties and 28 F1 progenies were evaluated in RBD with three replications during the year 2016-17 and 2017-18 at Experimental Farm of the Department of Crop Improvement, CSK HPKV, Palampur. Various cross combinations were also examined to study the genetics of resistance to powdery mildew and rust in linseed at Palampur and research station SAREC Kangra, respectively during the year 2017-18. The analysis of variance revealed significant differences among the genotypes for all the traits studied during 2016-17, 2017- 18 and pooled over environments, suggesting prevalence of wide range of genetic variability. Variance due to environment in pooled analysis revealed significant differences for all the traits studied except seeds per capsule. Genotype × environment (G × E) interaction was significant for most of the traits except plant height and technical height indicated a definite role of environment on the performance of genotypes. Variances due to GCA, SCA and their interaction with environment were significant for most of the traits. For most of the traits, there was preponderance of non-additive gene action, under such situation diallel selective mating or biparental mating in early segregating generations followed by recurrent selection might be appropriate approach toward genetic improvement of these traits. Based on GCA, Nagarkot and Surbhi were found to be good general combiner for seed yield whereas, JRF-1 and JRF-4 for fibre yield. On the basis of SCA effect and heterosis, the cross combinations Surbhi × Baner, Himani × JRF-4 and Bhagsu × Nagarkot for seed yield and Nagarkot × Himani for fibre yield were found best in pooled over the environments. The segregation pattern of powdery mildew and rust disease indicated that resistance was governed by a single dominant gene in cross combination T-397 × Nagarkot.
Open Access
Genetic improvement of linseed for inducing earliness in maturity and reducing the undesirable compounds through mutation
(Palampur, 2021-11-30) KUMAR, ARJUN; Paul, Satish
The present investigation entitled, “Genetic improvement of linseed for inducing earliness in maturity and reducing the undesirable compounds through mutation” was carried out in the experimental farm of Department of Genetics and Plant Breeding, COA, CSK HP KV, Palampur to assess the nature and magnitude of induced variability, determine the inheritance pattern of earliness in induced lines; determine the relative proportion of oil content of linseed mutants and isolate lines/mutants with reduced linolenic content in addition to earliness. Experimental material comprised of three linseed varieties viz. Baner, Him Alsi2 and Surbhi, seeds of which were treated with five doses of gamma rays (20, 30, 40, 50, 60 kR) and EMS (0.2 %, 0.4 %, 0.5 %, 0.6 %, 0.8 %) to generate M1 generation. On the basis of survival percentage in M1 generation under lab conditions the LD50 dose was estimated i.e. 96.63 kR (Baner), 72.94 kR (Him Alsi-2) and 53.22 kR (Surbhi) for gamma rays and 0.86 % (Baner), 0.72 % (Him Alsi- 2) and 0.35 % (Surbhi) for EMS. Plants harvested from M1 were carried forward to M2 generation. Both the mutagens were found effective and efficient in producing chlorophyll mutations and viable macromutations in M2 generation. Gamma rays and EMS also found effective in producing variability in range and mean values for different quantitative characters for which significant differences were obtained in both positive and negative directions. 113 mutants were carried forwarded to M3 generation based on their performance in M2 generation. Mean performance of M3 mutants showed significant shifts from their respective control. Analysis of variance was found significant for all the characters studied. Low GCV and PCV was found for number of days to 75 per cent maturity but high GCV (21.92 %) and PCV (27.05 %) was present in mutants for seed yield per plant. High heritability (96.52) coupled with low GAM (7.00 %) was found for number of days to 75 per cent maturity showing limited scope of its improvement, while high heritability (65.68 %) coupled with high GAM (36.60 %) was found for seed yield per plant showing presence of additive type of gene action. Line M3-3-5 showed highest oil percentage i.e. 58.44 per cent. The maximum reduction in linolenic acid percentage was found in M3-19-8 (36.11 %) followed by M3-19-4 (38.59 %) as compared to all the controls. Early maturing lines i.e. M3-14-2 (196.20 days) and M3-11-2 (196.20 days), high yielding line i.e. M3-13-2 (4.58 g) along with high oil content and low linolenic acid can be further evaluated to achieve the higher edible oil production of linseed
Open Access
Introgression of Cercospora leaf spot resistance into urdbean (Vigna mungo L. Hepper) employing various hybridization strategies by involving V. radiata, V. umbellata and V. angularis
(CSKHPKV Palampur, 2020-10-20) SHARMA, SHAILJA; Mital, Raj Kumar
Open Access
STUDIES ON GENETICS OF COLD STRESS TOLERANCE AND TRANSFERABILITY OF MARKERS FROM RELATED LEGUMES TO CHICKPEA (Cicer arietinum L.)
(CSKHPKV Palampur, 2020-10-27) THAKUR, SANCHIT; Jai Dev
The present investigation entitled “Studies on genetics of cold stress tolerance and transferability of markers from related legumes to chickpea (Cicer arietinum L.) was undertaken at experimental farm of the Department of Genetics & Plant Breeding, College of Agriculture, CSKHPKV, Palampur as well as at Mountain Agricultural Research and Extension Centre (MAREC), Sangla (off season). In the present study, the cold stress tolerant germplasm line ‘ICC16349’ was selected as a donor whereas, the cold susceptible parent ‘GPF2’ was used as recipient in hybridization. Crosses between germplasm lines ‘ICC16349’ and variety ‘GPF2’ of chickpea were attempted and resulting hybrids were screened for parental polymorphism using 51 SSR markers of which only one i.e TA180 showed parental polymorphism. Out of 80 F1 putative hybrids screened, only 34 were true hybrids. F1 generation was advanced to get F2 seed. F2 plants were phenotyped and morphological data were recorded. Days to germination, plant height and number of leaves gave good estimate of early plant vigour and data showed clear segregation of F2 plants into vigorous and non-vigorous plants. Chi-square test was applied which revealed that late flowering was governed by single dominant gene. Pod initiation followed a complex pattern of inheritance involving polygenes. Studies on transferability of SSR markers from related legumes to chickpea revealed that lentil SSR primers had highest transferability (36.36%) to chickpea followed by pea SSR primers (18.18%) and horsegram SSR primers (14.89%), respectively. Percent polymorphism was also found to be highest for lentil SSR primers (10.61%) followed by horsegram SSR primers (4.25%) and pea SSR primers (3.79%), respectively. In the present study, 62 new SSR markers were added to chickpea SSR primer pool from three legumes. The new SSR markers are expected to contribute to molecular breeding, germplasm characterization, diversity analysis, gene mapping and comparative genomic studies in chickpea.
Open Access
MOLECULAR CYTOGENETIC ANALYSIS OF RYE CHROMATIN INTROGRESSED BREAD WHEAT RECOMBINANTS FOR DROUGHT TOLERANCE AND YELLOW RUST RESISTANCE
(CSKHPKV, Palampur, 2019) Sharma, Parul; Chaudhary, H.K.
The prresentt rresearrch endeavorr was underrttaken tto accellerratte tthe wheatt iimprrovementt prrogrramme tthrrough devellopmentt,, dettecttiion and charractterriizattiion off rrye iinttrrogrressed brread wheatt rrecombiinantts att iin viittro and iin viivo llevell fforr drroughtt ttollerrance and yellllow rrustt rresiisttance.. Fiive ttrriittiicalles,, Hiimallayan rrye and varriious wheatt genottypes werre uttiilliized as tthe parrentts iin tthe wiide hybrriidiizattiion prrogrramme.. Among tthe diifffferrentt wheatt genottypes,, C--306,, VL--829 and Tyarrii--3 had hiighestt seed settttiing ffrrequency off 30..67 perr centt,, 27..27 perr centt and 17..41 perr centt,, rrespecttiivelly,, iin wheatt x rrye hybrriidiizattiion prrogrramme.. The ttrriittiicalle x wheatt and wheatt x rrye rrecombiinantts werre ffurrttherr subjjectted tto IImperatta cylliindriica–mediiatted chrromosome elliimiinattiion apprroach off doublled haplloiidy brreediing.. The varriiabiilliitty iin tthe haplloiid iinducttiion parrametterrs was obserrved tto be underr genettiic conttrroll fforr embrryo fforrmattiion and rregenerrattiion,, whiille pseudoseed fforrmattiion was onlly affffectted by auxiin ttrreattmentt.. Among tthe diifffferrentt generrattiions,, tthe backcrross generrattiions viiz..,, BC1F1 and BC1F2 werre ffound tto exhiibiitt siigniiffiicantt posiittiive rresponse ttowarrds haplloiid iinducttiion parrametterrs iin botth ttrriittiicalle x wheatt and wheatt x rrye hybrriidiizattiion.. The dettecttiion off new rrecombiinantts fforr iinttrrogrressiion off rrye chrromattiin tthrrough mollecullarr cyttogenettiic anallysiis rrevealled 1BL..1RS ttrransllocattiion iin 33 lliines ((55..93 perr centt)),, 1BL..1RS ttrransllocattiion allong wiitth substtiittuttiion iin 5 lliines ((8..47 perr centt)),, 1BL..1RS ttrransllocattiion allong wiitth addiittiion iin 8 lliines ((13..56 perr centt)),, substtiittuttiion off siinglle paiirr off rrye chrromosomes iin 5 lliines ((8..47 perr centt)) and addiittiion off rrye chrromattiin iin 1 lliine ((1..69 perr centt)).. Whiille 7 lliines werre ffound wiitth no rrye chrromattiin.. To valliidatte tthe effffectt off rrye iinttrrogrressiion on drroughtt ttollerrance and yellllow rrustt rresiisttance,, allll 59 rrecombiinantts werre evalluatted underr wellll watterred and watterr sttrressed condiittiions att iin viivo and iin viittro llevell.. Anallysiis off varriiance rrevealled suffffiiciientt genettiic varriiabiilliitty among genottypes fforr diifffferrentt ttrraiitts att botth iin viivo and iin viittro llevells.. Underr ffiielld condiittiions,, diirrectt iinfflluence off rrye iinttrrogrressiion was obserrved on pllantt heiightt,, grraiin yiielld,, biiollogiicall yiielld and harrvestt iindex.. The scrreeniing fforr drroughtt ttollerrance underr iin viittro condiittiions rrevealled posiittiive siigniiffiicantt effffectt off rrye iinttrrogrressiions on gerrmiinattiion perrcenttage,, shoott llengtth,, rroott llengtth,, rroott numberr,, colleopttiille llengtth and seedlliing viigourr iindex.. Overrallll,, 1BL..1RS ttrransllocattiions and substtiittuttiions werre ffound tto exhiibiitt siigniiffiicantt iinfflluence on mostt off tthe drroughtt parrametterrs.. The scrreeniing fforr yellllow rrustt rresiisttance rrevealled diifffferrenttiiall diisease rreacttiion,, wherre 52 rrecombiinantts werre obserrved tto be rresiisttantt.. The lliines exhiibiittiing superriiorriitty fforr tthe ttrraiitts can be used as a parrenttall sourrce iin wheatt iimprrovementt endeavorrs.

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