Genetic improvement of linseed for inducing earliness in maturity and reducing the undesirable compounds through mutation
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Date
2021-11-30
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Palampur
Abstract
The present investigation entitled, “Genetic improvement of linseed for inducing
earliness in maturity and reducing the undesirable compounds through mutation” was carried
out in the experimental farm of Department of Genetics and Plant Breeding, COA, CSK HP
KV, Palampur to assess the nature and magnitude of induced variability, determine the
inheritance pattern of earliness in induced lines; determine the relative proportion of oil
content of linseed mutants and isolate lines/mutants with reduced linolenic content in addition
to earliness. Experimental material comprised of three linseed varieties viz. Baner, Him Alsi2 and Surbhi, seeds of which were treated with five doses of gamma rays (20, 30, 40, 50, 60
kR) and EMS (0.2 %, 0.4 %, 0.5 %, 0.6 %, 0.8 %) to generate M1 generation. On the basis of
survival percentage in M1 generation under lab conditions the LD50 dose was estimated i.e.
96.63 kR (Baner), 72.94 kR (Him Alsi-2) and 53.22 kR (Surbhi) for gamma rays and 0.86 %
(Baner), 0.72 % (Him Alsi- 2) and 0.35 % (Surbhi) for EMS. Plants harvested from M1 were
carried forward to M2 generation. Both the mutagens were found effective and efficient in
producing chlorophyll mutations and viable macromutations in M2 generation. Gamma rays
and EMS also found effective in producing variability in range and mean values for different
quantitative characters for which significant differences were obtained in both positive and
negative directions. 113 mutants were carried forwarded to M3 generation based on their
performance in M2 generation. Mean performance of M3 mutants showed significant shifts
from their respective control. Analysis of variance was found significant for all the characters
studied. Low GCV and PCV was found for number of days to 75 per cent maturity but high
GCV (21.92 %) and PCV (27.05 %) was present in mutants for seed yield per plant. High
heritability (96.52) coupled with low GAM (7.00 %) was found for number of days to 75 per
cent maturity showing limited scope of its improvement, while high heritability (65.68 %)
coupled with high GAM (36.60 %) was found for seed yield per plant showing presence of
additive type of gene action. Line M3-3-5 showed highest oil percentage i.e. 58.44 per cent. The
maximum reduction in linolenic acid percentage was found in M3-19-8 (36.11 %) followed by
M3-19-4 (38.59 %) as compared to all the controls. Early maturing lines i.e. M3-14-2 (196.20
days) and M3-11-2 (196.20 days), high yielding line i.e. M3-13-2 (4.58 g) along with high oil
content and low linolenic acid can be further evaluated to achieve the higher edible oil
production of linseed