Molecular diversity analysis and biochemical profiling of Sri Lankan and Indian tea (Camellia sinensis (L.) O. Kuntze) genotypesMolecular diversity analysis and biochemical profiling of Sri Lankan and Indian tea (Camellia sinensis (L.) O. Kuntze) genotypes
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Date
2022-06-27
Journal Title
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Publisher
palampur
Abstract
The present investigation entitled, “Molecular diversity analysis and biochemical
profiling of Sri Lankan and Indian tea (Camellia sinensis (L.) O. Kuntze) genotypes”
was undertaken with the objectives of molecular and biochemical characterization of
Sri Lankan and Indian tea germplasm and identification of potential genotypes to
expedite its genetic improvement. Total of 171 tea genotypes comprising 94 Sri
Lankan and 77 Indian genotypes were used to assess molecular diversity. Based on 16
morphological characters, first three principal components explained 57.61 % and
58.43 % of the total variability of Sri Lankan and Indian genotypes, respectively.
Young shoot colour recorded the highest Shannon’s index (H’=1.14) and (H’=1.16)
among morphological descriptors of Sri Lankan and Indian genotypes, respectively.
Based on biochemical characterization, genotypes viz. PBGT35, PBGT48, PBGT53
and PBGT71 recorded low caffeine content. Total of 28 polymorphic SSRs i.e.10
unigenic SSRs and 18 functionally relevant SSR amplified 94 different alleles and
were developed for molecular diversity and population structure analysis. Based on
the SSR data of 171 accessions, all Sri Lankan genotypes were grouped in a single
cluster whereas Indian genotypes were grouped in two distinct clusters through
structure analysis and principle coordinate analysis (PCoA). Five core collections
were developed by different methods. Based on the evaluation parameters, the core
collection derived using Gower’s method was the most representative method of the
initial collections of Sri Lanka and India. Among selected 21 Sri Lankan core
genotypes, 11 accessions belong to introductions followed by 5 TRI developed
cultivars and 5 estate selections. Among selected 18 Indian core genotypes, 11
accessions are of China types followed by 2 Assam types and 5 recommended
cultivars.