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Subject: Veterinary Public Health ×

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Now showing 1 - 9 of 56
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    ThesisItemOpen Access
    DETECTION AND MOLECULAR CHARACTERIZATION OF ENTEROCOCCUS SPECIES FROM POULTRY MEAT
    (Department of Veterinary Public Health & Epidemiology College of Veterinary Science and Animal Husbandry Anand Agricultural University Anand, 2021) Parmar Nilam Nareshbhai; Dr. M. N. Brahmbhatt
    The present study demonstrated the detection and molecular characterization of Enterococcus species in poultry meat. Enterococci are associated with food spoiling, especially animal origin food. The involvement of enterococci in human disease has raised concerns about food safety.
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    ThesisItemOpen Access
    EPIDEMIOLOGY, MOLECULAR CHARACTERIZATION AND COMPARISON OF DIAGNOSTIC TEST FOR RABIES VIRUS
    (DEPARTMENT OF VETERINARY PUBLIC HEALTH & EPIDEMIOLOGY COLLEGE OF VETERINARY SCIENCE AND ANIMAL HUSBANDRY ANAND AGRICULTURAL UNIVERSITY ANAND, 2021) Jeetendrakumar H. Chaudhary; Dr. M. N. Brahmbhatt
    Rabies is one of the ancient recognized lethal encephalitis caused by the rabies lyssavirus affecting almost all kinds of mammals, all over the world. The organism is a neurotropic, enveloped, single stranded, negative sense RNA virus belonging to the family Rhabdoviridae and genus Lyssavirus under the order Mononegavirales. In India, majority of human rabies deaths are due to dog bites and therefore pre-exposure immunization of dogs is of utmost importance to prevent the spread of rabies to other animals. Correct and prompt diagnosis of rabies is also important for the postexposure prophylaxis/measures in human and animals. Study of rabies dynamics in the field is required to understand the genetic variations currently in circulation and their evolutionary adaptation and modifications thereby to help devise effective control measures.
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    ThesisItemOpen Access
    COMPARISON OF LOOP MEDIATED ISOTHERMAL AMPLIFICATION WITH POLYMERASE CHAIN REACTION FOR DETECTION OF SALMONELLA SPP. IN POULTRY MEAT
    (Department of Veterinary Public Health & Epidemiology College of Veterinary Science and Animal Husbandry Anand Agricultural University Anand, 2020) Pargi Zalak Bharatkumar; Dr. J. B. Nayak
    The present study was conducted with aim to isolate and identify Salmonella species from poultry meat samples collected from retail meat markets in and around Anand, confirming Salmonella spp. isolates by polymerase chain reaction (PCR) and loop mediated isothermal amplification (LAMP), antimicrobial resistance pattern of isolates by disc diffusion method and comparing LAMP with PCR method by checking their sensitivity and specificity for detection of Salmonella species.
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    ThesisItemOpen Access
    “COMPARISON OF LOOP MEDIATED ISOTHERMAL AMPLIFICATION WITH POLYMERASE CHAIN REACTION FOR DETECTION OF STAPHYLOCOCCUS AUREUS & METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS IN CHEVON
    (Department of Veterinary Public Health & Epidemiology College of Veterinary Science and Animal Husbandry Anand Agricultural University Anand, 2020) Sonali Thakur; Dr. M. N. Brahmbhatt
    The present study demonstrated the comparison of Polymerase Chain Reaction (PCR) with Loop Mediated Isothermal Amplification (LAMP) for detection of Staphylococcus aureus (S. aureus) and Methicillin Resistant Staphylococcus aureus (MRSA) in chevon. Total 150 raw chevon samples were collected from retail meat shops in and around Anand city. These samples were collected under aseptic conditions and then transferred to the laboratory on ice for further processing.
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    ThesisItemOpen Access
    ISOLATION AND MOLECULAR CHARACTERIZATION OF EXTENDED SPECTRUM BETA LACTAMASE PRODUCING ESCHERICHIA COLI FROM MILK
    (Department of Veterinary Public Health & Epidemiology College of Veterinary Science and Animal Husbandry Anand Agricultural University Anand, 2019) PAGHDAR DHARABEN M.; Dr. J. B. NAYAK
    The present study was carried out in the post graduate laboratory, Department of Veterinary Public Heath and Epidemiology, College of Veterinary Science and Animal Husbandary, A.A.U., Anand in order to isolation and molecular characterization of Extended Beta Lactamase Producing Escherichia coli from milk to study their cultural characteristic and to judge the hygienic level of different local dairy cattle farms in and around Anand, Gujarat. A total of 150 samples were collected from different dairy cattle farms located in Navali (25), Sai dairy farm (50), Chikhodara (25), Bedva (25) and Mogar (25). All the samples were first inoculated on MacConkey Agar (MCA) and isolates showing pink colonies (lactose fermenting) were further transferred to Eosin Methylene Blue Agar (EMB) for confirmation and greenish metallic sheen producing isolates were confirmed as E. coli isolates.
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    ThesisItemOpen Access
    ISOLATION AND MOLECULAR CHARACTERIZATION OF EXTENDED SPECTRUM BETA LACTAMASE PRODUCING ESCHERICHIA COLI FROM CHICKEN
    (Department of Veterinary Public Health and Epidemiology College of Veterinary Science and Animal Husbandry Anand Agricultural University, Anand, 2019) Gida Harpalbhai; Dr. M. N. Brahmbhatt
    This study was undertaken in order to isolate and molecularly characterize extended spectrum beta lactamase producing Escherichia coli from chicken, to check their prevalence and to judge hygienic level of different local chicken butcher’s and retail market meat in and around anand. Total 150 chicken muscle samples were collected from chicken shops in and around Anand under aseptic precautions in sterile screw lid sample collector for further processing and microbiological analysis. Samples were collected aseptically in sterile test screw capped vials and immediately transferred to the laboratory for processing and bacteriological investigation.
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    ThesisItemOpen Access
    DETECTION OF TETRACYCLINE ANTIBIOTICS IN MILK OF ANAND DISTRICT
    (COLLEGE OF VETERINARY SCIENCE AND ANIMAL HUSBANDRY ANAND AGRICULTURAL UNIVERSITY ANAND, 2017) Mistry Urvish Pravinbhai; Dr. M. N. Brahmbhatt
    This study was undertaken with the objective of determining the overall presence of the Tetracycline antibiotic residues in raw and pasteurized milk samples collected from different localities of Anand district, Gujarat. Establishing sensitivity and specificity of immuno-photometric test and comparing the method with traditional chromatographic techniques for residue detection was the complementary objective. The research work was done at Department of Veterinary Public Health, College of Veterinary Science and Animal Husbandry, AAU, Anand and Food Quality Testing Laboratory, College of Food Processing Technology and Bio-Energy, AAU, Anand.
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    ThesisItemOpen Access
    SEROPREVALENCE OF BRUCELLOSIS IN SMALL RUMINANTS AND HUMANS OF ANAND DISTRICT
    (DEPARTMENTOF VETERINARY PUBLIC HEALTH AND EPIDEMIOLOGY COLLEGE OF VETERINARY SCIENCE AND ANIMAL HUSBANDRY ANAND AGRICULTURAL UNIVERSITY ANAND, 2017) RADHABEN R. PADHER; DR. J. B. NAYAK
    The present study was carried out at the post graduate laboratory, Department of Veterinary Public Health and Epidemiology, College of Veterinary Science and Animal Husbandry, A.A.U., Anand with the intention to determine the seroprevalence of brucellosis among small ruminants and human beings of Anand district, Gujarat employing RBPT, STAT and I-ELISA and compared the efficacy of these tests.
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    ThesisItemOpen Access
    ISOLATION AND CHARACTERIZATION OF AEROMONAS SPECIES FROM POULTRY MEAT
    (AAU, Anand, 2009) SMITA; Brahmbhatt, M. N.
    The aim of the present study was to isolate, identify and characterize Aeromonas species from poultry meat. A total of 120 samples were processed for estimating prevalence oi Aeromonas spp. Out of those 120 samples, 66 isolates oi Aeromonas were identified. On the basis of biochemical characterization 47 isolates of A. sobria, 11 isolates of A. hydrophila and 8 isolates of A. caviae were detected. When the source wise study of Aeromonas was conducted it was found that maximum number of Aeromonas isolates were recovered from heart (87.5 per cent) followed by liver (66.66 per cent), thigh muscle and chest muscle (40 per cent each). When different selective culture media were evaluated for isolation of Aeromonas spp. from poultry meat it was found that percent recovery of Aeromonas isolates were more from Ampicillin Dextrin Agar (89.39 per cent), followed by Aeromonas Starch DNAse agar (68.18 per cent) and Aeromonas isolation media (18.18 per cent). Specificity of PCR assay for detection of A. hydrophila and A. sobria was performed by testing against different gram positive and gram negative organisms.Primers were found to be specific for A. hydrophila and A. sobria. All the isolates of A. sobria (47) and A. hydrophila (11) which were identified on the basis of biochemical characterization were subjected to PCR and confirmed. All 66 Aeromonas isolates were tested for presence of aerolysin, haemolysin and enterotoxin gene. None of the isolate showed presence of aerolysin and enterotoxin while overall prevalence of haemolysin gene was 78.78 per cent. In A. hydrophila, 54.54 per cent; A. caviae, 37.5 per cent and in A. sobria, 91.48 per cent isolates were found to possess haemolysin gene. All the isolates of Aeromonas were subjected to antimicrobial drug sensitivity test against gentamicin, chloramphenicol, ciprofloxacin, kanamycin, bacitracin, rifampicin, tetracycline and erythromycin. Maximum sensitivity pattern was recorded with chloramphenicol (86.36per cent), gentamicin (81.82 per cent), ciprofloxacin (60.61 per cent), kanamycin (34.85 per cent), bacitracin (25.76 per cent), tetracycline (16.67 per cent), rifampicin (15.15 per cent) and erythromycin (9.09 per cent). The resistance pattern of Aeromonas isolated from chicken meat to various antibiotics was observed as bacitracin (74.24 per cent) followed by rifampicin (71.21 per cent), kanamycin (65.15 per cent), erythromycin (53.03 per cent), tetracycline (33.33 per cent), ciprofloxacin (22.73 per cent) and gentamicin (18.18 per cent). It was concluded that overall prevalence of Aeromonas spp. in poultry meat was 55 per cent which is a matter of concern from public health point of view and needs proper attention. The overall prevalence of 78.78 per cent of Aeromonas isolates showing presence of haemolysin gene (Virulence gene) in poultry meat is also a matter of concern and this study reveals that poultry meat could be a potential threat to public health. Hence, more attention for implementation of HACCP concept from food safety point of view is required. Antibiotic sensitivity pattern of Aeromonas isolates revealed that chloramphenicol was found to be most effective drug (86.36per cent) followed by gentamicin (81.82 per cent), ciprofloxacin (60.61 per cent), kanamycin (34.85 per cent), bacitracin (25.76 per cent), tetracycline (16.67 per cent), rifampicin (15.15 per cent) and erythromycin (9.09 per cent).