ISOLATION AND MOLECULAR CHARACTERIZATION OF EXTENDED SPECTRUM BETA LACTAMASE PRODUCING ESCHERICHIA COLI FROM MILK
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Date
2019
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Department of Veterinary Public Health & Epidemiology College of Veterinary Science and Animal Husbandry Anand Agricultural University Anand
Abstract
The present study was carried out in the post graduate laboratory, Department of Veterinary Public Heath and Epidemiology, College of Veterinary Science and Animal Husbandary, A.A.U., Anand in order to isolation and molecular characterization of Extended Beta Lactamase Producing Escherichia coli from milk to study their cultural characteristic and to judge the hygienic level of different local dairy cattle farms in and around Anand, Gujarat.
A total of 150 samples were collected from different dairy cattle farms located in Navali (25), Sai dairy farm (50), Chikhodara (25), Bedva (25) and Mogar (25). All the samples were first inoculated on MacConkey Agar (MCA) and isolates showing pink colonies (lactose fermenting) were further transferred to Eosin Methylene Blue Agar (EMB) for confirmation and greenish metallic sheen producing isolates were confirmed as E. coli isolates.