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2009 - 200912010 - 201816
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Subject: Plant Biotechnology ×

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Now showing 1 - 9 of 17
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    ThesisItemOpen Access
    Elucidation of Genes Involved in Scopoletin Biosynthesis in Convolvulus microphyllus Sieb. ex Spreng. by Transcriptome Sequencing
    (DEPARTMENT OF AGRICULTURAL BIOTECHNOLOGY ANAND AGRICULTURAL UNIVERSITY ANAND, 2018) Rafaliya Rutul V.; Dr. Subhash N.
    Shankhpushpi (Convolvulus microphyllus Sieb. ex Spreng.) is an indigenous and very significant herb that consider as a gift of nature in Ayurveda. It is a natural medicine which enhances the memory power due to the presence of its valuable secondary metabolite “scopoletin”. In present investigation, a total 4 plant parts viz., leaf, root, stem and flower of Anand local genotype were used for biochemical study using LC/MS chromatography at 30, 45, 60 and 90 days after sowing (DAS). The highest scopoletin content was measured in stem (0.0732%) and root (0.0476%) tissue at 90 DAS and lowest content was measured in root (0.0283%) and stem (0.0110%) tissue at 45 DAS. For further molecular analysis, the minimum and maximum scopoletin containing tissues viz., stem and root at 45 and 90 DAS of Anand local shankhpushpi genotype was used.
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    ThesisItemOpen Access
    Next Generation Sequencing based identification and classification of TLCV (Tomato Leaf Curl Virus) isolates from different Agroclimatic zones of Gujarat
    (DEPARTMENT OF AGRICULTURAL BIOTECHNOLOGY B. A. COLLEGE OF AGRICULTURE ANAND AGRICULTURAL UNIVERSITY ANAND, 2018) Bhatt Kunj D.; Dr. Akarsh Parihar
    The present investigation entitled “Next Generation Sequencing based identification and classification of TLCV (Tomato Leaf Curl Virus) isolates from different Agro-climatic zones of Gujarat” was carried out at the Department of Agricultural Biotechnology, Anand Agricultural University, Anand during 2016-18.
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    ThesisItemOpen Access
    EVALUATION AND VALIDATION OF HOUSEKEEPING GENES FOR GENE EXPRESSION STUDIES IN CLUSTER BEAN [Cyamopsis tetragonoloba L. (Taub.)] UNDER DROUGHT STRESS CONDITION
    (Department of Agricultural Biotechnology Anand Agricultural University Anand, 2018) Patel Samarth R.; Dr. R. S. Fougat
    Cluster bean is botanically named as Cyamopsis tetragonoloba L. (Taub.) and belongs to family Leguminosae (Fabaceae). It is an annual self pollinated legume crop. Guar is one of the most important and potential vegetable cum industrial crop grown for its tender pods for vegetable purpose and seeds for endospermic galactomannon gum. Tropical Africa is its probable centre of origin as wild species were found to occur in that region. Due to deep rooted system, this short duration, hardy and drought resilient legume is extremely adapted to the inhospitable environment of rainfed regions. Drought is the most important abiotic factor limiting growth and thus yield adversely affect. The present investigation was carried out with an aim to study the evaluation and validation of housekeeping genes for gene expression studies in cluster bean within susceptible and resistant genotypes through RT-PCR.
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    ThesisItemOpen Access
    Identification of Genes involved during wilt disease caused by Fusarium oxysporum f. sp. cumini by transcriptome sequencing in Cumin (Cuminum cyminum L.)
    (DEPARTMENT OF AGRICULTURAL BIOTECHNOLOGY B. A. COLLEGE OF AGRICULTURE ANAND AGRICULTURAL UNIVERSITY ANAND, 2018) Poonam; Dr. Y. M. Shukla
    Cumin (Cuminum cyminum L.) (2n = 2x = 14) one of the oldest seed spices, is assumed to be an inhabitant from the east Mediterranean to east India and economically most important spices after black pepper. It is popularly being used as spice for their distinct aroma and is used to add in other food preparations. Production constrains due to quite a few biotic stresses of which wilt disease are the most severe caused by Fusarium oxysporum f. sp. cumini. The aim of this research was to elucidate the molecular mechanism underpinning the defence related genes conferring wilt resistance in cumin, which constitutes an important step forward for progression and suitable intervention stratagem.
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    ThesisItemOpen Access
    IN SILICO IDENTIFICATION OF microRNAs AND THEIR VALIDATION IN PEARL MILLET [Pennisetum glaucum L.]
    (DEPARTMENT OF AGRICULTURAL BIOTECHNOLOGY B. A. COLLEGE OF AGRICULTURE ANAND AGRICULTURAL UNIVERSITY ANAND, 2016) Amit Kumar; Dr.R. S. Fougat
    The endogenous small non-coding functional microRNAs (miRNAs) are short in size, ranging from ~21 to 24 nucleotides in length and play a pivotal role in gene expression in plants and animals by negative regulation or silencing of genes either by cleavage or blocking of translation of homologous mRNA. Although various high throughput and expensive techniques like forward genetics and direct cloning are employed to detect miRNAs in plants but comparative genomics complemented with novel bioinformatic tools paced the way for efficient and cost-effective identification of miRNAs through homologous sequence search with previously known miRNAs.
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    ThesisItemOpen Access
    “Assessment of genetic diversity in Aloe barbadensis Mill. using molecular markers
    (Anand Agricultural University, Anand, 2009) PUSHPA MOTIRAM DEORE; Dr. SANGHAMITRA SAMANTARAY
    Aloe barbadensis Mill. is one of the important medicinal plants of India which is frequently being used in herbal medicine. Genetic analysis using RAPD and ISSR profiling of A. barbadensis accessions available at Directorate of Medicinal and Aromatic plants Research (DMAPR), Boriavi, Anand was carried out for estimating the extent of diversity and establishing phylogenetic relationship
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    ThesisItemOpen Access
    “PHENOTYPIC AND MOLECULAR CHARACTERIZATION OF BACTERIAL BLIGHT OF RICE PATHOGEN (Xanthomonas oryzae pv. oryzae) FROM GUJARAT
    (Anand Agricultural University, Anand, 2011) PATEL FENILKUMAR SURESHBHAI; Dr. Subhash N.
    Bacterial blight (BB) is the most destructive disease of rice. BB is caused by bacteria Xanthomonas oryzae pv. oryzae. The disease is known to occur in epidemic proportions in many parts of the world, incurring severe crop loss up to 50%. Bacteria show variation in their characteristics according to region, environmental condition, soil type and availability of the host. In order to know the diversity in Xanthomonas oryzae pv. oryzae, it is necessary to characterize the bacterial isolates collected from various parts of Gujarat.
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    ThesisItemOpen Access
    “Identification and validation of internal control genes for quantitative gene expression studies in pearl millet under biotic stress (downy mildew infection) and abiotic stress (salt stress
    (Anand Agricultural University, Anand, 2012) POONAM D. KANANI; Dr. Y. M. Shukla
    Pearl millet (Pennisetum glauccum (L.) R. Br.) is an annual, cross pollinating, monocot crop belonging to the family Poaceae and sub family Penicedae. It is the sixth most important cereal of the world and has critical value as food security in some of the world’s hottest and driest cultivated areas of Africa and Asia. However, the crop faces constraints to the productivity which includes the prevalence of different biotic and abiotic stresses. The present work was carried out with an aim to identify and select endogenous genes for pearlmillet under biotic stress (downy mildew) and abiotic stress (salinity) and validating constitutively expressed genes in pearlmillet through RT-PCR and analyzed it through geNorm for best stable reference gene
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    ThesisItemOpen Access
    VALIDATION OF ENDOGENOUS GENES IN RICE (Oryza sativa L.) UNDER DROUGHT AND SALT STRESS”
    (Anand Agricultural University, Anand, 2012) ANUCHITHRA V.L.; Dr. Subhash N.
    The use of suitable and stable reference genes for normalization is an important and unavoidable step during gene expression studies in relative quantification using Real Time PCR. Validation of endogenous genes in rice (Oryza sativa L.) under drought and salt stress was carried out with an aim to identify most suitable reference genes for the normalization of gene expression data in rice. After four days of drought and salinity stress the resistant cultivars showed higher RWC as compared to the susceptible cultivars. The drought susceptible cultivar, GR 3 showed higher leaf rolling index (LRI) as compared to the resistant cultivar AAUDR 1. Based on the visual symptoms of salt damage the tolerant cultivar Dandi scored 3 in the Standard Evaluation System (SES) and susceptible cultivar GR 11 scored 7, indicating tolerance and susceptible reactions, respectively. cDNA preparation was carried out from total RNA of rice seedlings