IN SILICO IDENTIFICATION OF microRNAs AND THEIR VALIDATION IN PEARL MILLET [Pennisetum glaucum L.]
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Date
2016
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DEPARTMENT OF AGRICULTURAL BIOTECHNOLOGY B. A. COLLEGE OF AGRICULTURE ANAND AGRICULTURAL UNIVERSITY ANAND
Abstract
The endogenous small non-coding functional microRNAs (miRNAs) are short
in size, ranging from ~21 to 24 nucleotides in length and play a pivotal role in gene
expression in plants and animals by negative regulation or silencing of genes either by
cleavage or blocking of translation of homologous mRNA. Although various high
throughput and expensive techniques like forward genetics and direct cloning are
employed to detect miRNAs in plants but comparative genomics complemented with
novel bioinformatic tools paced the way for efficient and cost-effective identification
of miRNAs through homologous sequence search with previously known miRNAs.
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