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M. Sc. Dissertations

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20101
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Subject: Animal Production Technology ×
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    ThesisItemOpen Access
    Role of prostaglandin PGF2α on the survivability and acrosomal integrity of frozen-thawed semen of murrah buffalo-bulls
    (CCSHAU, 2010) Nain, Surender; Pardeep Singh
    Semen of six Murrah buffalo bulls was used in this investigation. Approximately 200 mini straws of frozen semen from each bull were taken. On the day of experiment 25-30 straws of a bull’s frozen semen were taken from the cryocan and thawed at 37° C for 30 seconds. After thawing semen was transferred to a 10 ml test tube kept at 37° C. Five small test tubes labeled as control, 10, 20, 30 and 40 were kept at 37º C in an incubator. In each test tube 900 μl of thawed semen was transferred. In control tube 100 μl of freshly prepared Tris buffer and in other four tubes 100 μl of PGF2α having concentrations of 10, 20, 30 and 40 μg/ml respectively was added. All five tubes were kept in incubator whose temperature was maintained at 37º C for three hours. After one hour semen from each tube was evaluated for progressive sperm motility, live and dead spermatozoa, abnormal sperms and spermatozoa with intact acrosomes. On completion of two hours of incubation only progressive sperm motility of semen was evaluated. After three hours semen was again evaluated for progressive sperm motility, live and dead spermatozoa, abnormal sperms and spermatozoa with intact acrosomes. The study revealed that addition of PGF2α.at a concentration of 20μg/ml of frozen thawed semen of buffalo-bulls significantly maintained higher sperm motility at each hours of incubation. Livability of the spermatozoa was also significantly higher at 1st and 3rd hours of incubation with the addition of PGF2α @ 20μg/ml as compared to control and other concentrations. Spermatozoa with percent intact acrosomes were significantly higher with the addition of 20μg/ml PGF2α as compared to control and other concentrations at 1st and 3r d hours of incubation. Percent abnormal spermatozoa were significantly less with the addition of different concentration of PGF2α. So it can be concluded from this study that addition of PGF2α at a concentration of 20μg/ml maintains higher sperm motility, livability and more spermatozoa with intact acrosomes up to three hours of incubation of frozen-thawed semen of Murrah buffalo-bulls.