Role of prostaglandin PGF2α on the survivability and acrosomal integrity of frozen-thawed semen of murrah buffalo-bulls
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Date
2010
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Publisher
CCSHAU
Abstract
Semen of six Murrah buffalo bulls was used in this investigation. Approximately 200 mini
straws of frozen semen from each bull were taken. On the day of experiment 25-30 straws of a bull’s
frozen semen were taken from the cryocan and thawed at 37° C for 30 seconds. After thawing semen
was transferred to a 10 ml test tube kept at 37° C. Five small test tubes labeled as control, 10, 20, 30
and 40 were kept at 37º C in an incubator. In each test tube 900 μl of thawed semen was transferred.
In control tube 100 μl of freshly prepared Tris buffer and in other four tubes 100 μl of PGF2α having
concentrations of 10, 20, 30 and 40 μg/ml respectively was added. All five tubes were kept in
incubator whose temperature was maintained at 37º C for three hours. After one hour semen from
each tube was evaluated for progressive sperm motility, live and dead spermatozoa, abnormal
sperms and spermatozoa with intact acrosomes. On completion of two hours of incubation only
progressive sperm motility of semen was evaluated. After three hours semen was again evaluated for
progressive sperm motility, live and dead spermatozoa, abnormal sperms and spermatozoa with
intact acrosomes. The study revealed that addition of PGF2α.at a concentration of 20μg/ml of frozen
thawed semen of buffalo-bulls significantly maintained higher sperm motility at each hours of
incubation. Livability of the spermatozoa was also significantly higher at 1st and 3rd hours of
incubation with the addition of PGF2α @ 20μg/ml as compared to control and other concentrations.
Spermatozoa with percent intact acrosomes were significantly higher with the addition of 20μg/ml
PGF2α as compared to control and other concentrations at 1st and 3r d hours of incubation. Percent
abnormal spermatozoa were significantly less with the addition of different concentration of PGF2α.
So it can be concluded from this study that addition of PGF2α at a concentration of 20μg/ml
maintains higher sperm motility, livability and more spermatozoa with intact acrosomes up to three
hours of incubation of frozen-thawed semen of Murrah buffalo-bulls.
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Keywords
Livestock, Biological phenomena, Concentrates, Additives, Alkaloids, Poultry equipment, Freezing, Organic compounds, Fertilizers, Thawing