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M. Sc. Dissertations

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2010 - 20113
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End date: 2011 × Start date: 2010 × Type: Thesis × Thesis Type: M.V.Sc ×
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    ThesisItemOpen Access
    Role of prostaglandin PGF2α on the survivability and acrosomal integrity of frozen-thawed semen of murrah buffalo-bulls
    (CCSHAU, 2010) Nain, Surender; Pardeep Singh
    Semen of six Murrah buffalo bulls was used in this investigation. Approximately 200 mini straws of frozen semen from each bull were taken. On the day of experiment 25-30 straws of a bull’s frozen semen were taken from the cryocan and thawed at 37° C for 30 seconds. After thawing semen was transferred to a 10 ml test tube kept at 37° C. Five small test tubes labeled as control, 10, 20, 30 and 40 were kept at 37º C in an incubator. In each test tube 900 μl of thawed semen was transferred. In control tube 100 μl of freshly prepared Tris buffer and in other four tubes 100 μl of PGF2α having concentrations of 10, 20, 30 and 40 μg/ml respectively was added. All five tubes were kept in incubator whose temperature was maintained at 37º C for three hours. After one hour semen from each tube was evaluated for progressive sperm motility, live and dead spermatozoa, abnormal sperms and spermatozoa with intact acrosomes. On completion of two hours of incubation only progressive sperm motility of semen was evaluated. After three hours semen was again evaluated for progressive sperm motility, live and dead spermatozoa, abnormal sperms and spermatozoa with intact acrosomes. The study revealed that addition of PGF2α.at a concentration of 20μg/ml of frozen thawed semen of buffalo-bulls significantly maintained higher sperm motility at each hours of incubation. Livability of the spermatozoa was also significantly higher at 1st and 3rd hours of incubation with the addition of PGF2α @ 20μg/ml as compared to control and other concentrations. Spermatozoa with percent intact acrosomes were significantly higher with the addition of 20μg/ml PGF2α as compared to control and other concentrations at 1st and 3r d hours of incubation. Percent abnormal spermatozoa were significantly less with the addition of different concentration of PGF2α. So it can be concluded from this study that addition of PGF2α at a concentration of 20μg/ml maintains higher sperm motility, livability and more spermatozoa with intact acrosomes up to three hours of incubation of frozen-thawed semen of Murrah buffalo-bulls.
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    ThesisItemOpen Access
    Utilization of citrus fruit waste for development of chicken meat roll
    (CCSHAU, 2011) Mahender Singh; Sharma, D.P.
    A study was conducted to assess the utilit y of kinnow (Citrus reticulata) waste in development of chicken meat rolls. Fresh peel and pulp of kinnow were incorporated at 5, 7.5 and 10% levels each. Chemical, physico-chemical, microbiological and sensory characteristic were evaluated. Product was stored at 4±1oC for shelf life study. Peel had higher crude fibre and polyphenol content than pulp. Addition of peel and pulp increased the moisture, crude fibre and polyphenol content of chicken meat rolls. As a result of moisture retention ability of fibre, cooking loss and shear press value of treated chicken rolls decreased. Chicken rolls with peel and pulp showed lower residual nitrite level. Peel, due to its higher fibre and polyphenolic content was more effective in improving the processing characteristics of chicken rolls. Peel was acceptable organoleptically up to 5% and pulp up to 10% level of addition. Control and treated products (5% peel and 10% pulp added rolls) had overall acceptability scores of 7.00 and above up to 12th day of storage. Peel and pulp addition was able to check the rise in TBA value in treated products during storage. All the products were microbiologically acceptable up to 12th day. It is concluded that 5% peel and 10% pulp of kinnow in chicken meat rolls can be incorporated for improving the fibre and polyphenol content and processing characteristics of chicken meat rolls. However, peel and pulp in dried form may yield better result than fresh form, for further increasing the fibre content of meat product up to substantial level.
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    ThesisItemOpen Access
    Microbiological, cytological and ultrasonographic studies in infertile mares and their therapeutic management
    (CCSHAU, 2011) Assad, Nafis Ibni; Bugalia, N.S.
    Infertility in broodmares due to uterine infection and associated endometritis occurs following breeding and foaling, and cripples annual foal production and accounts for heavy economic loss in the equine industry. Both diagnosis and therapeutic management as early as possible are warranted to maintain optimum fertility. Present study was therefore undertaken with the aim to evaluate the diagnostic utility of uterine cytology, uterine culture and ultrasonography in detection of uterine infection in infertile mares and to recommend a combination of diagnostic techniques to be employed as a routine procedure in the management of equine uterine infections and associated endometritis. The therapeutic efficacy of different treatment regimens in management of uterine infection in infertile mares was also evaluated. Investigation was conducted on 27 broodmares of equine breeding stud (RVC)-Hisar. Mares were divided into four groups (Group I, II and III, with 7 infertile mares in each group; Group IV with 6 fertile mares). All the mares were subjected to pre-treatment assessment of reproductive status by uterine ultrasonography, uterine cytology and uterine culture, followed by therapeutic management of uterine infections and associated endometritis with three different therapeutic regimens for three different groups. Transrectal ultrasound was done using a linear array probe (5MHz and 7 MHz). Sampling for uterine cytology and uterine cultures was done using low-volume uterine flush technique. Group I mares were treated with saline uterine lavage + PGF2α + intrauterine antibiotic infusion, Group II mares received saline uterine lavage + Cloprostenol + intrauterine antibiotic infusion, Group III mares were treated with saline uterine lavage + Oxytocin + intrauterine antibiotic infusion. Group IV mares received no treatment. The antibiotics used were based on the in vitro antibiotic sensitivity test. Further, therapeutic response was evaluated by post-treatment ultrasound conducted between days 7-10 after treatment and post-treatment conception was recorded following 1st, 2nd and 3rd inseminations. The sensitivity, specificity, positive predictive value and negative predictive value of ultrasound, uterine cytology & uterine culture were recorded to be 86.66%, 50.00%, 68.42% & 75.00%; 71.42%, 69.23%,71.4% & 69.23% and 100%,100%, 100% & 100% respectively. The results of all the techniques were highly and significantly correlated. Uterine cultural examination of the flush sample was the most reliable method for detection of uterine infection in infertile mares. Uterine culture and uterine cytology was found to be the best combination for routine use in the diagnosis of infections. However, uterine ultrasonography may be used for evaluation of quantity of uterine fluid as well as assessment of endometrial oedema/uterine wall thickening in prediction of fertility. Therapeutic regimens involving Cloprostenol and Oxytocin in addition to uterine lavage + intrauterine antibiotic infusion were found to be first and second preference treatment protocols, respectively, in management of uterine infections in infertile mares.