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M. Sc. Dissertations

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20101
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Subject: Molecular Biology and Biotechnology × Author: Asha Rani × End date: 2016 ×
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    ThesisItemOpen Access
    Development of interspecific hybrids between Indian mustard (Brassica juncea Coss. & czern) and white mustard (Brassica alba Moench) through embryo rescue
    (CCSHAU, 2010) Asha Rani; Yadav, R.C.
    Wide hybridization is an important method of incorporation of desirable character. The present study was undertaken to develop an interspecific hybrids between B. juncea cv. RH30, cv. RH8812 and B. alba to incorporate the Alternaria blight resistant character from B. alba to B. juncea. Ovules were excised from siliqua and cultured on seven different media supplemented with different growth regulators. In cv.RH30 X B. alba,the hybrid siliquae were 1.2 to 3 cm in length whereas in case of parent plants of cv. RH30, siliquae length was between 4.1 to 4.5 cm. In cv. RH8812 X B. alba, the hybrid siliquae ranged from 2.8 to 3.4 cm in length while in parent plants of cv. RH8812, the siliquae length was between 4.9 to 5.7 cm. In cross of RH30 X B. alba and RH8812 X B. alba, 48% and 39% pod setting was observed respectively. In case of cv. RH30 X B. alba, highest percent response for ovule germination (79.46%) was observed on MS medium supplemented with kinetin (2.5mg/l) and CH (0.5g/l). In case of Brassica juncea cv. RH8812 X B. alba, highest response (45.63%) of ovule germination was on MS + BAP (2.5 mg/l) + CH (0.5mg/l). Six rooting media were tried for root formation in regenerated shoots. Maximum rooting response was 100% on MS medium supplemented with IAA (0.5mg/l) in both the hybrids. The regenerated hybrid plants were transferred to a mixture of sand: soil in 1:1 ratio in pots and 80 percent hybrid plants survived in the soil.