Development of interspecific hybrids between Indian mustard (Brassica juncea Coss. & czern) and white mustard (Brassica alba Moench) through embryo rescue
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Date
2010
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Publisher
CCSHAU
Abstract
Wide hybridization is an important method of incorporation of desirable character. The present
study was undertaken to develop an interspecific hybrids between B. juncea cv. RH30, cv. RH8812 and
B. alba to incorporate the Alternaria blight resistant character from B. alba to B. juncea. Ovules were
excised from siliqua and cultured on seven different media supplemented with different growth
regulators. In cv.RH30 X B. alba,the hybrid siliquae were 1.2 to 3 cm in length whereas in case of
parent plants of cv. RH30, siliquae length was between 4.1 to 4.5 cm. In cv. RH8812 X B. alba, the
hybrid siliquae ranged from 2.8 to 3.4 cm in length while in parent plants of cv. RH8812, the siliquae
length was between 4.9 to 5.7 cm. In cross of RH30 X B. alba and RH8812 X B. alba, 48% and 39%
pod setting was observed respectively. In case of cv. RH30 X B. alba, highest percent response for
ovule germination (79.46%) was observed on MS medium supplemented with kinetin (2.5mg/l) and CH
(0.5g/l). In case of Brassica juncea cv. RH8812 X B. alba, highest response (45.63%) of ovule
germination was on MS + BAP (2.5 mg/l) + CH (0.5mg/l). Six rooting media were tried for root
formation in regenerated shoots. Maximum rooting response was 100% on MS medium supplemented
with IAA (0.5mg/l) in both the hybrids. The regenerated hybrid plants were transferred to a mixture of
sand: soil in 1:1 ratio in pots and 80 percent hybrid plants survived in the soil.
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Keywords
Nitrogen, Planting, Yields, Zinc, Maize, Fertilizers, Biological development, Drying, Biological phenomena, Dry matter