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20101
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Subject: Molecular Biology and Biotechnology × Author: Aneja, Bharti × End date: 2010 × Type: Thesis ×
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    ThesisItemOpen Access
    Micronutrient and molecular diversity analysis in mungbean [Vigna radiata (L.) wilczek] genotypes
    (CCSHAU, 2010) Aneja, Bharti; Yadav, Neelam R.
    Vigna radiata (L.) Wilczek, commonly called mungbean or greengram is the third most important pulse crop of India occupying nearly 3 million hectare. It is an excellent source of inexpensive and easily digestible proteins of low flatulence and is available in several edible forms. Iron and Zinc are important micronutrients for human health whose deficiency causes anemia and malfunctioning of immune system. Commercial cultivars contain low levels of iron and zinc and it is important to assess genetic variability in available germplasm for improving micronutrient content in commercial cultivars. The present study was undertaken to study SRAP polymorphism among 21 mungbean genotypes using 29 SRAP primers. A total of 121 amplified bands were produced which were polymorphic with an average of 4.65 bands per primer. The size of amplified bands ranged from 70-3000 bp. Unique bands were displayed by 8 mungbean genotypes using SRAP analysis. Six out of 29 SRAP primers were most useful in fingerprinting mungbean genotypes under study. The similarity coefficients between different genotypes ranged from 0.45-0.96 with an average similarity value of 0.71. At an arbitrary cut-off at 60 per cent similarity level on a dendrogram, the mungbean accessions were categorized into two major clusters. ML1108 and 2KM115 were found to genetically similar. SMH99-1A and ML776 showed high iron and zinc content while Satya was poor in iron as well as zinc content. Recombinant inbred lines involving ML776 and Satya could be used for tagging gene for micronutrient content. The results indicated that SRAP markers were efficient for identification of Vigna radiata genotypes and for determination of the genetic relationships among them.