Micronutrient and molecular diversity analysis in mungbean [Vigna radiata (L.) wilczek] genotypes
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Date
2010
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CCSHAU
Abstract
Vigna radiata (L.) Wilczek, commonly called mungbean or greengram is the third most
important pulse crop of India occupying nearly 3 million hectare. It is an excellent source of
inexpensive and easily digestible proteins of low flatulence and is available in several edible forms. Iron
and Zinc are important micronutrients for human health whose deficiency causes anemia and
malfunctioning of immune system. Commercial cultivars contain low levels of iron and zinc and it is
important to assess genetic variability in available germplasm for improving micronutrient content in
commercial cultivars. The present study was undertaken to study SRAP polymorphism among 21
mungbean genotypes using 29 SRAP primers. A total of 121 amplified bands were produced which
were polymorphic with an average of 4.65 bands per primer. The size of amplified bands ranged from
70-3000 bp. Unique bands were displayed by 8 mungbean genotypes using SRAP analysis. Six out of
29 SRAP primers were most useful in fingerprinting mungbean genotypes under study. The similarity
coefficients between different genotypes ranged from 0.45-0.96 with an average similarity value of
0.71. At an arbitrary cut-off at 60 per cent similarity level on a dendrogram, the mungbean accessions
were categorized into two major clusters. ML1108 and 2KM115 were found to genetically similar.
SMH99-1A and ML776 showed high iron and zinc content while Satya was poor in iron as well as zinc
content. Recombinant inbred lines involving ML776 and Satya could be used for tagging gene for
micronutrient content. The results indicated that SRAP markers were efficient for identification of
Vigna radiata genotypes and for determination of the genetic relationships among them.
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Keywords
Fertigation, Irrigation, Developmental stages, Rice, Yields, Fertilizers, Trickle irrigation, Grain, Irrigation methods, Productivity