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M. Sc. Dissertations

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Subject: Chemistry × Author: Mamta Rani × End date: 2009 × Start date: 2004 ×
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    ThesisItemOpen Access
    Chemical composition of stem of kinnow mandarin (Citrus reticulata)
    (CCSHAU, 2009) Mamta Rani; M. Khabiruddin
    Kinnow mandarin (Citrus nobilis × Citrus deliciosa) belongs to the family Rutaceae is a variety of citrus fruit. It is cultivated widely in tropical and subtropical regions. It has special importance due to their multifold nutritional and medicinal values. It is known for its antimicrobial, insecticidal and medicinal properties. Stems of Citrus reticulata were collected from the Department of Horticulture, CCS HAU, Hisar and were chopped into small pieces and then extracted with hot methanol. The extractives were column chromtographed over silica gel and five compounds were obtained. 10 Compound A (Tricosane) was obtained on elution with benzene:hexane (1:19). Compound B (Campesterol) was obtained on elution with ethyl acetate – benzene (1:9). Compound C (β-sitosterol) was obtained on elution with ethyl acetate-benzene (1:5). Compound D [5,7,3,4'-Tetrahydroxyflavone (Kaempferol)] was obtained on elution with ethyl acetate–benzene (1:3). Compound E (β-sitosterol- β-D-glucoside) was obtained on elution with ethyl acetate:benzene (2:1). The characterization of isolated compounds was carried out on the basis of UV-visible, IR and 1H NMR data and other properties of the compounds. The methanolic extract of stem of Citrus reticulata was tested for antifungal activity at 2000, 4000, 6000, 8000 and 10000 ppm against Fusarium solani and Rhizoctonia solani and percent inhibition was recorded. It was found to have inhibitory effect on both the pathogens (F. solani and R. solani). The area of per cent inhibition was more in F. solani in comparison to R. solani and the maximum inhibition (about 100%) of both the pathogens was at 10000 ppm.