Chemical composition of stem of kinnow mandarin (Citrus reticulata)
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Date
2009
Authors
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Journal ISSN
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Publisher
CCSHAU
Abstract
Kinnow mandarin (Citrus nobilis × Citrus deliciosa) belongs to the family
Rutaceae is a variety of citrus fruit. It is cultivated widely in tropical and subtropical
regions. It has special importance due to their multifold nutritional and medicinal
values. It is known for its antimicrobial, insecticidal and medicinal properties. Stems
of Citrus reticulata were collected from the Department of Horticulture, CCS HAU,
Hisar and were chopped into small pieces and then extracted with hot methanol. The
extractives were column chromtographed over silica gel and five compounds were
obtained.
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Compound A (Tricosane) was obtained on elution with benzene:hexane (1:19).
Compound B (Campesterol) was obtained on elution with ethyl acetate – benzene
(1:9). Compound C (β-sitosterol) was obtained on elution with ethyl acetate-benzene
(1:5). Compound D [5,7,3,4'-Tetrahydroxyflavone (Kaempferol)] was obtained on
elution with ethyl acetate–benzene (1:3). Compound E (β-sitosterol- β-D-glucoside)
was obtained on elution with ethyl acetate:benzene (2:1).
The characterization of isolated compounds was carried out on the basis of
UV-visible, IR and 1H NMR data and other properties of the compounds.
The methanolic extract of stem of Citrus reticulata was tested for antifungal
activity at 2000, 4000, 6000, 8000 and 10000 ppm against Fusarium solani and
Rhizoctonia solani and percent inhibition was recorded. It was found to have
inhibitory effect on both the pathogens (F. solani and R. solani). The area of per cent
inhibition was more in F. solani in comparison to R. solani and the maximum
inhibition (about 100%) of both the pathogens was at 10000 ppm.
Description
Keywords
Genotypes, Animal developmental stages, Cowpeas, Biological phenomena, Cotton, Land resources, Selection, Phenols, Organic acids, Sampling